中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
6期
534-536
,共3页
孙婷婷%崔瑛%汪晶%杨晶晶%张月月%赵华伟%王桁杰
孫婷婷%崔瑛%汪晶%楊晶晶%張月月%趙華偉%王桁傑
손정정%최영%왕정%양정정%장월월%조화위%왕항걸
肉豆蔻挥发油%抗抑郁作用%悬尾实验%强迫游泳实验%单胺类神经递质
肉豆蔻揮髮油%抗抑鬱作用%懸尾實驗%彊迫遊泳實驗%單胺類神經遞質
육두구휘발유%항억욱작용%현미실험%강박유영실험%단알류신경체질
Volatile oil of Myristica fragrans Houtt%Antidepressant effect%Tail suspension tests%Forced swimming tests%Monoamine neurotransmitters
目的 观察肉豆蔻挥发油对行为绝望抑郁小鼠的影响并探讨其作用机制.方法 健康雄性昆明种小鼠分为对照组、盐酸氟西汀组、肉豆蔻挥发油高、中、低剂量组,连续灌胃(ig)7d,末次给药0.5 h后通过悬尾实验、强迫游泳实验研究肉豆蔻挥发油的抗抑郁作用,采用酶联免疫法测定小鼠脑内5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)的含量.结果 与对照组[(82.60±24.70)s]比较,肉豆蔻挥发油中剂量组小鼠悬尾不动的时间[(54.40±15.87)s]显著降低,差异具有统计学意义(P＜0.05);肉豆蔻挥发油中剂量组小鼠脑内5-HT、DA、NE的含量分别为(19.35±2.79) ng/ml、(12.16±0.71) pg/ml、(0.27±0.12)pg/ml,对照组小鼠脑内三种递质的含量分别为(14.95±4.83) ng/ml、(11.32±0.95) pg/ml、(0.20±0.11)ng/ml,中剂量组5-HT含量显著增高,差异具有统计学意义(P＜0.05).结论 肉豆蔻挥发油有抗抑郁作用,可能与提高小鼠脑内5-HT、DA、NE的含量有关.
目的 觀察肉豆蔻揮髮油對行為絕望抑鬱小鼠的影響併探討其作用機製.方法 健康雄性昆明種小鼠分為對照組、鹽痠氟西汀組、肉豆蔻揮髮油高、中、低劑量組,連續灌胃(ig)7d,末次給藥0.5 h後通過懸尾實驗、彊迫遊泳實驗研究肉豆蔻揮髮油的抗抑鬱作用,採用酶聯免疫法測定小鼠腦內5-羥色胺(5-HT)、多巴胺(DA)、去甲腎上腺素(NE)的含量.結果 與對照組[(82.60±24.70)s]比較,肉豆蔻揮髮油中劑量組小鼠懸尾不動的時間[(54.40±15.87)s]顯著降低,差異具有統計學意義(P＜0.05);肉豆蔻揮髮油中劑量組小鼠腦內5-HT、DA、NE的含量分彆為(19.35±2.79) ng/ml、(12.16±0.71) pg/ml、(0.27±0.12)pg/ml,對照組小鼠腦內三種遞質的含量分彆為(14.95±4.83) ng/ml、(11.32±0.95) pg/ml、(0.20±0.11)ng/ml,中劑量組5-HT含量顯著增高,差異具有統計學意義(P＜0.05).結論 肉豆蔻揮髮油有抗抑鬱作用,可能與提高小鼠腦內5-HT、DA、NE的含量有關.
목적 관찰육두구휘발유대행위절망억욱소서적영향병탐토기작용궤제.방법 건강웅성곤명충소서분위대조조、염산불서정조、육두구휘발유고、중、저제량조,련속관위(ig)7d,말차급약0.5 h후통과현미실험、강박유영실험연구육두구휘발유적항억욱작용,채용매련면역법측정소서뇌내5-간색알(5-HT)、다파알(DA)、거갑신상선소(NE)적함량.결과 여대조조[(82.60±24.70)s]비교,육두구휘발유중제량조소서현미불동적시간[(54.40±15.87)s]현저강저,차이구유통계학의의(P＜0.05);육두구휘발유중제량조소서뇌내5-HT、DA、NE적함량분별위(19.35±2.79) ng/ml、(12.16±0.71) pg/ml、(0.27±0.12)pg/ml,대조조소서뇌내삼충체질적함량분별위(14.95±4.83) ng/ml、(11.32±0.95) pg/ml、(0.20±0.11)ng/ml,중제량조5-HT함량현저증고,차이구유통계학의의(P＜0.05).결론 육두구휘발유유항억욱작용,가능여제고소서뇌내5-HT、DA、NE적함량유관.
Objective To observe the antidepressant effect of the volatile oil of Myristica in mice and investigate its mechanism.Methods Healthy male Kunming mice were divided into control group,fluoxetine hydrochloride group,high,medium and low dose group,and all of the mice were gavaged for 7 days.The role of antidepressant on the mice were observed with tail suspension tests and forced swimming tests.Then serotonin(5-HT),dopamine(DA) and norepinephrine (NE) in the brain of the mice were determined by enzyme-linked immunosorbent assay.Results Compared with the control group((82.60 ± 24.70)s),the medium dose group could shorten the immobility time of the tail suspension tests ((54.40± 15.87) s),and showed statistical significance (P＜ 0.05).The content of 5-HT,DA,NE in the brain of the medium group were (19.35±2.79) ng/ml,(12.16±0.71)pg/ml,(0.27±0.12) ng/ml,and control group were (14.95±4.83) ng/ml,(11.32±0.95) pg/ml,(0.20±0.11) ng/ml.Compared with the control group,the content of 5-HT of the medium dose group was increased and showed statistical significance(P＜0.05).Conclusion The volatile oil of Myristica fragrans Houtt has antidepressant effect,and may be related to raising the content of 5-HT,DA,NE in the brain of the mice,especially 5-HT.
