中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
7期
580-583
,共4页
袁苏涛%杜越群%孟涛%林玲
袁囌濤%杜越群%孟濤%林玲
원소도%두월군%맹도%림령
帕金森病%褪黑素%褪黑素受体
帕金森病%褪黑素%褪黑素受體
파금삼병%퇴흑소%퇴흑소수체
Parkinson's disease%Melatonin%Melatonin receptors
目的 观察偏侧帕金森病(PD)大鼠中脑黑质毁损侧(右侧)及健侧(左侧)多巴胺及其代谢产物、以及褪黑素和褪黑素受体的变化.方法 6-羟基多巴胺(6-OHDA)损毁大鼠右侧黑质-纹状体通路制备偏侧PD模型,大鼠分为溶剂对照组和PD组.分别于建模后3周,6周10:00和22:00分离黑质组织,用高效液相色谱检测多巴胺及其代谢产物、以及褪黑素水平的变化,采用RT-PCR,Western-Blot检测PD大鼠毁损侧及健侧黑质褪黑素受体MT1和MT2的表达水平.结果 PD大鼠毁损侧黑质与其健侧相比,建模后3周和6周多巴胺水平分别降低了83％和88％;建模后6周10:00和22:00,PD大鼠毁损侧黑质与其健侧相比,褪黑素水平分别增加了22％和29％.与溶剂对照组双侧黑质相比,PD大鼠健侧黑质褪黑素水平分别增加了46％和31％,毁损侧MT1和MT2的mRNA水平分别降低了91.04％和90.24％ (P＜0.01),蛋白水平分别降低了58％和70％ (P＜0.01).结论 6-OHDA诱导的晚期PD大鼠双侧黑质组织褪黑素水平增加,以及毁损侧黑质褪黑素受体表达下调可能与PD的进展有关.
目的 觀察偏側帕金森病(PD)大鼠中腦黑質燬損側(右側)及健側(左側)多巴胺及其代謝產物、以及褪黑素和褪黑素受體的變化.方法 6-羥基多巴胺(6-OHDA)損燬大鼠右側黑質-紋狀體通路製備偏側PD模型,大鼠分為溶劑對照組和PD組.分彆于建模後3週,6週10:00和22:00分離黑質組織,用高效液相色譜檢測多巴胺及其代謝產物、以及褪黑素水平的變化,採用RT-PCR,Western-Blot檢測PD大鼠燬損側及健側黑質褪黑素受體MT1和MT2的錶達水平.結果 PD大鼠燬損側黑質與其健側相比,建模後3週和6週多巴胺水平分彆降低瞭83％和88％;建模後6週10:00和22:00,PD大鼠燬損側黑質與其健側相比,褪黑素水平分彆增加瞭22％和29％.與溶劑對照組雙側黑質相比,PD大鼠健側黑質褪黑素水平分彆增加瞭46％和31％,燬損側MT1和MT2的mRNA水平分彆降低瞭91.04％和90.24％ (P＜0.01),蛋白水平分彆降低瞭58％和70％ (P＜0.01).結論 6-OHDA誘導的晚期PD大鼠雙側黑質組織褪黑素水平增加,以及燬損側黑質褪黑素受體錶達下調可能與PD的進展有關.
목적 관찰편측파금삼병(PD)대서중뇌흑질훼손측(우측)급건측(좌측)다파알급기대사산물、이급퇴흑소화퇴흑소수체적변화.방법 6-간기다파알(6-OHDA)손훼대서우측흑질-문상체통로제비편측PD모형,대서분위용제대조조화PD조.분별우건모후3주,6주10:00화22:00분리흑질조직,용고효액상색보검측다파알급기대사산물、이급퇴흑소수평적변화,채용RT-PCR,Western-Blot검측PD대서훼손측급건측흑질퇴흑소수체MT1화MT2적표체수평.결과 PD대서훼손측흑질여기건측상비,건모후3주화6주다파알수평분별강저료83％화88％;건모후6주10:00화22:00,PD대서훼손측흑질여기건측상비,퇴흑소수평분별증가료22％화29％.여용제대조조쌍측흑질상비,PD대서건측흑질퇴흑소수평분별증가료46％화31％,훼손측MT1화MT2적mRNA수평분별강저료91.04％화90.24％ (P＜0.01),단백수평분별강저료58％화70％ (P＜0.01).결론 6-OHDA유도적만기PD대서쌍측흑질조직퇴흑소수평증가,이급훼손측흑질퇴흑소수체표체하조가능여PD적진전유관.
Objective To observe the changes of dopamine and its metabolites,melatonin,as well as melatonin receptors in the substantia nigras (SN) in 6-OHDA induced hemiparkinsonian rats.Methods PD rat was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta and medial forebrain bundle.Rats were divided into PD groups and vehicle groups,and sacrificed at 10:00 and 22:00 respectively,at 3 and 6 weeks post-lesion.The contents of dopamine and its metabolites as well as melatonin were measured by HPLC.The expressions of MT1 and MT2 mRNA and protein were detected by RT-PCR and Western-Blot respectively.Results Dopamine levels declined by 83％ and 88％ respectively at 3 and 6 weeks post-lesion in the injected side of PD rats,compared with the contralateral side.Melatonin levels in the injected side increased by 22％ and 29％ respectively.However,melatonin increased by 46％ and 31％ respectively at 10:00 and 22:00,at 6 weeks post-lesion in the contralateral side,compared with the same side of the vehicle.The expressions of MT1 and MT2 receptors mRNA were significantly decreased by 91.04％ and 90.24％ respectively in comparison with the vehicle,while the protein expression of MT1 and MT2 receptors significantly declined by 58％ and 70％ respectively.Conclusion Increased melatonin and down-regulated MT1 and MT2 receptors in the lesioned SN in 6-OHDA-induced rats may be associated with the progression of PD.
