CAJ | 학술논문

目的观察大鼠液压冲击脑损伤Toll样受体4(TLR4)表达变化,探讨其与神经功能的关系.方法成年雄性SD大鼠56只,随机分为损伤组(n=48)和假手术组(n=8).采用液压冲击制备颅脑损伤模型,术后1h、6h、12h、24 h、3d、7d对动物进行神经功能评分,并用干湿重法、免疫组化法和Western Blot 分别测定水肿脑组织含水量及TLR4蛋白表达.结果神经功能评分6h出现下降[(3.86±0.42)分],24 h下降至最低值[(2.65±0.32)分],3d逐渐升高[(3.25±0.17)分];免疫组化和Western Blot均显示水肿脑组织TLR4的表达均6h开始增高,24h至3d达到高峰,7d后降低.线性相关性分析发现TLR4表达与神经功能评分呈显著负相关(r1=-0.824,rw=-0.867,P＜0.05).结论大鼠颅脑液压冲击伤TLR4出现高表达,可能诱发了继发性炎性脑损伤导致神经功能障碍.
목적 관찰대서액압충격뇌손상Toll양수체4(TLR4)표체변화,탐토기여신경공능적관계.방법 성년웅성SD대서56지,수궤분위손상조(n=48)화가수술조(n=8).채용액압충격제비로뇌손상모형,술후1h、6h、12h、24 h、3d、7d대동물진행신경공능평분,병용간습중법、면역조화법화Western Blot 분별측정수종뇌조직함수량급TLR4단백표체.결과 신경공능평분6h출현하강[(3.86±0.42)분],24 h하강지최저치[(2.65±0.32)분],3d축점승고[(3.25±0.17)분];면역조화화Western Blot균현시수종뇌조직TLR4적표체균6h개시증고,24h지3d체도고봉,7d후강저.선성상관성분석발현TLR4표체여신경공능평분정현저부상관(r1=-0.824,rw=-0.867,P＜0.05).결론 대서로뇌액압충격상TLR4출현고표체,가능유발료계발성염성뇌손상도치신경공능장애.
Objective To investigate the expression of Toll-like receptor 4 (TLR4),and explore its relationship with neurological function after fluid percussion brain injury in rats.Methods 56 adult rats were randomly divided into traumatic brain injury group(TBI group,n=48) and sham operation group(SO group,n=8).The experimental models were established.The water content of edematous brain and the expression of TLR4 were measured with dry-wet measure,immunohistochemistry and Western Blot at 1 h,6 h,12 h,24 h,3 d,7 d after shock respectively.Results Compared with SO group,neuronal function score decreased in TBI group from 6 h(3.86±0.42),reached to the lowest level at 24 h(2.65±0.32),and gradually rose at 3rd day (3.25±0.17).TLR4 immunoreactive expression increased from 6 h,reached its maxmum at 24 h,lasted to 3rd day,and then began to drop at 7th day.The linear regression analysis indicated that expression of TLR4 had negative correlation with change of neuronal function score (r 1 =-0.824,r w =-0.867,P＜0.05).Conclusion TLR4 expression is upregulated following fluid percussion injury in rats and involved in neurological function impairment by inducing secondary inflammatory brain injury.