中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
7期
587-590
,共4页
余意%韩金珠%连阳阳%韩金红%郝伟%张瑞岭
餘意%韓金珠%連暘暘%韓金紅%郝偉%張瑞嶺
여의%한금주%련양양%한금홍%학위%장서령
酒精依赖%细胞骨架蛋白%肌动蛋白结合蛋白%细胞周期素依赖性蛋白激酶5
酒精依賴%細胞骨架蛋白%肌動蛋白結閤蛋白%細胞週期素依賴性蛋白激酶5
주정의뢰%세포골가단백%기동단백결합단백%세포주기소의뢰성단백격매5
Alcohol dependence%Cytoskeletal protein%Cofilin%Cyclin-dependent kinase-5
目的 探讨长期酒精暴露和戒断对大鼠不同脑区肌动蛋白结合蛋白(cofilin)、磷酸化cofilin (P-cofilin)以及细胞周期素依赖性蛋白激酶5(cdk5)表达的影响.方法 雄性SD大鼠24只,随机等分为1个对照组和3个实验组.实验组大鼠自由饮含低浓度乙醇(体积分数为6％)的水溶液2月,对照组大鼠正常饮自来水.2月后撤除酒精进行戒断症状评分,并分别在戒断0h、戒断6h、戒断2d处死大鼠取脑组织.采用免疫组织化学方法检测各组大鼠伏隔核和纹状体区cofilin、p-cofilin (ser3)以及cdk5的表达水平.结果 实验组大鼠撤除酒精后出现明显的戒断症状,在戒断6h达到高峰.在大鼠伏隔核区,戒断0h组cofilin表达水平较对照组显著降低[(0.31±0.05),(0.39±0.05),P＜0.05],戒断0h、戒断6h组cdk5表达水平较对照组显著升高[(0.36±0.07)、(0.34±0.07),(0.25±0.05),P＜0.05].在大鼠纹状体,戒断0h组cofilin表达水平较对照组显著降低[(0.26±0.04),(0.34±0.05),P＜0.05],戒断6h组p-cofilin表达水平较对照组显著升高[(0.43±0.06),(0.30±0.06),P＜0.01],戒断0h组cdk5表达水平较对照组显著升高[(0.35±0.06),(0.26±0.05),P＜0.05],戒断6h组cdk5表达水平与对照组相比显著升高[(0.37±0.06),(0.26±0.05),P＜0.01].结论 长期酒精暴露诱导大鼠形成酒精依赖,并导致相关脑区肌动蛋白结合蛋白以及cdk5的表达变化.
目的 探討長期酒精暴露和戒斷對大鼠不同腦區肌動蛋白結閤蛋白(cofilin)、燐痠化cofilin (P-cofilin)以及細胞週期素依賴性蛋白激酶5(cdk5)錶達的影響.方法 雄性SD大鼠24隻,隨機等分為1箇對照組和3箇實驗組.實驗組大鼠自由飲含低濃度乙醇(體積分數為6％)的水溶液2月,對照組大鼠正常飲自來水.2月後撤除酒精進行戒斷癥狀評分,併分彆在戒斷0h、戒斷6h、戒斷2d處死大鼠取腦組織.採用免疫組織化學方法檢測各組大鼠伏隔覈和紋狀體區cofilin、p-cofilin (ser3)以及cdk5的錶達水平.結果 實驗組大鼠撤除酒精後齣現明顯的戒斷癥狀,在戒斷6h達到高峰.在大鼠伏隔覈區,戒斷0h組cofilin錶達水平較對照組顯著降低[(0.31±0.05),(0.39±0.05),P＜0.05],戒斷0h、戒斷6h組cdk5錶達水平較對照組顯著升高[(0.36±0.07)、(0.34±0.07),(0.25±0.05),P＜0.05].在大鼠紋狀體,戒斷0h組cofilin錶達水平較對照組顯著降低[(0.26±0.04),(0.34±0.05),P＜0.05],戒斷6h組p-cofilin錶達水平較對照組顯著升高[(0.43±0.06),(0.30±0.06),P＜0.01],戒斷0h組cdk5錶達水平較對照組顯著升高[(0.35±0.06),(0.26±0.05),P＜0.05],戒斷6h組cdk5錶達水平與對照組相比顯著升高[(0.37±0.06),(0.26±0.05),P＜0.01].結論 長期酒精暴露誘導大鼠形成酒精依賴,併導緻相關腦區肌動蛋白結閤蛋白以及cdk5的錶達變化.
목적 탐토장기주정폭로화계단대대서불동뇌구기동단백결합단백(cofilin)、린산화cofilin (P-cofilin)이급세포주기소의뢰성단백격매5(cdk5)표체적영향.방법 웅성SD대서24지,수궤등분위1개대조조화3개실험조.실험조대서자유음함저농도을순(체적분수위6％)적수용액2월,대조조대서정상음자래수.2월후철제주정진행계단증상평분,병분별재계단0h、계단6h、계단2d처사대서취뇌조직.채용면역조직화학방법검측각조대서복격핵화문상체구cofilin、p-cofilin (ser3)이급cdk5적표체수평.결과 실험조대서철제주정후출현명현적계단증상,재계단6h체도고봉.재대서복격핵구,계단0h조cofilin표체수평교대조조현저강저[(0.31±0.05),(0.39±0.05),P＜0.05],계단0h、계단6h조cdk5표체수평교대조조현저승고[(0.36±0.07)、(0.34±0.07),(0.25±0.05),P＜0.05].재대서문상체,계단0h조cofilin표체수평교대조조현저강저[(0.26±0.04),(0.34±0.05),P＜0.05],계단6h조p-cofilin표체수평교대조조현저승고[(0.43±0.06),(0.30±0.06),P＜0.01],계단0h조cdk5표체수평교대조조현저승고[(0.35±0.06),(0.26±0.05),P＜0.05],계단6h조cdk5표체수평여대조조상비현저승고[(0.37±0.06),(0.26±0.05),P＜0.01].결론 장기주정폭로유도대서형성주정의뢰,병도치상관뇌구기동단백결합단백이급cdk5적표체변화.
Objective To investigate the effects of chronic ethanol exposure and withdrawal on the expression of actin-binding protein cofilin,p-cofilin and cyclin-dependent kinase-5 (cdk5) in the nucleus accumbens and striatum in rat brain.Methods Twenty-four male SD rats were randomly divided into one control group and three experimental groups.In the experimental groups,ethanol was administered in drinking water at the concentration of 6％ (V/V) for two months.Rats in control group drank normal drinking water.After two months ethanol was removed and ethanol withdrawal syndromes were evaluated.Rats were sacrificed on withdrawal 0 h,withdrawal 6 h and withdrawal 2 d.The expression levels of cofilin,p-cofilin(ser3)and cdk5 in the rat brain were measured by immunohistochemistry methods.Results Withdrawal syndrome scores of ethanol fed rats were obviously higher than those of control rats after ethanol was removed,the highest score occurred at 6 h after ethanol withdrawal.In the nucleus accumbens area of rat brain,the levels of cofilin on withdrawal 0 h significantly decreased compared with control group ((0.31±0.05),(0.39± 0.05),P＜ 0.05).The levels of cdk5 on withdrawal 0 h and withdrawal 6 h significantly increased compared with control group((0.36±0.07),(0.34±0.07),(0.25±0.05),P＜0.05).In the striatum of rat brain,the levels of cofilin on withdrawal 0 h significantly decreased compared with control group ((0.26±0.04),(0.34±0.05),P＜0.05).The levels of p-cofilin on withdrawal 6 h significantly increased compared with control group((0.43±0.06),(0.30±0.06),P＜0.01).The levels of cdk5 on withdrawal 0 h significantly increased compared with control group((0.35±0.06),(0.26±0.05),P＜0.05),and the levels of cofilin on withdrawal 6 h significantly decreased compared with control group((0.37±0.06),(0.26±0.05),P＜0.01).Conclusion Chronic ethanol exposure can induce the development of ethanol dependence,and it accompanies with changes in the expression of actin-binding protein and cdk5 in the brain of rats.
