中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
7期
591-593
,共3页
买买提热厦提·吐尔逊%马晓洁%张文惠%夏小龙%桂学萍
買買提熱廈提·吐爾遜%馬曉潔%張文惠%夏小龍%桂學萍
매매제열하제·토이손%마효길%장문혜%하소룡%계학평
奥氮平%肥胖大鼠%脂肪源性细胞因子%认知功能损害
奧氮平%肥胖大鼠%脂肪源性細胞因子%認知功能損害
오담평%비반대서%지방원성세포인자%인지공능손해
Olanzapine%Obese rat%Adipose-derived cytokine%Cognitive impairment
目的 观察奥氮平诱导肥胖大鼠糖脂代谢紊乱对脂肪源性细胞因子影响和认知功能损伤实验研究.方法 40只大鼠随机分为对照组和实验组,每组20只.对照组大鼠进行普通饲料喂养,实验组在普通饲粮喂养基础上行奥氮平(1.2 mg·kg-1)灌胃4周建立奥氮平诱导肥胖大鼠模型.采用酶联免疫测定法测定2组大鼠血清中肿瘤坏死因子α(TNF-α),白细胞介素6(IL-6)和C-反应蛋白(CRP)的含量,生化比色法测定血清葡萄糖(FBS)含量.采用Y-型迷宫观察2组大鼠学习、记忆能力和逃避潜伏期变化.结果 灌胃4周后,与对照组比较,奥氮平组大鼠的体质量、血糖、血脂均显著升高,差异有统计学意义(P＜0.05);奥氮平组大鼠的血清TNF-α、IL-6和CRP分别为(1.57±0.04) ng/ml、(127.47± 11.38) pg/ml和(2.68±0.06) mg/ml,与对照组比较[(0.59±0.03)ng/ml、(96.58±8.77) pg/ml和(1.86±0.04) mg/ml]有不同程度的升高,差异有统计学意义(P＜0.05).实验组大鼠电击次数和逃避潜伏期均高于对照组(P＜0.05).FBS分别与hs-CRP、IL-6和TNF-α呈正相关(r=0.385,0.260,1.280;均P＜0.05).结论 奥氮平可以导致大鼠糖脂代谢紊乱,血清TNF-α,IL-6和CRP分泌增加,TNF-α、IL-6和CRP浓度变化与FBS水平呈正相关.血糖升高可促进细胞因子细胞毒性作用,导致大鼠认知功能损害.
目的 觀察奧氮平誘導肥胖大鼠糖脂代謝紊亂對脂肪源性細胞因子影響和認知功能損傷實驗研究.方法 40隻大鼠隨機分為對照組和實驗組,每組20隻.對照組大鼠進行普通飼料餵養,實驗組在普通飼糧餵養基礎上行奧氮平(1.2 mg·kg-1)灌胃4週建立奧氮平誘導肥胖大鼠模型.採用酶聯免疫測定法測定2組大鼠血清中腫瘤壞死因子α(TNF-α),白細胞介素6(IL-6)和C-反應蛋白(CRP)的含量,生化比色法測定血清葡萄糖(FBS)含量.採用Y-型迷宮觀察2組大鼠學習、記憶能力和逃避潛伏期變化.結果 灌胃4週後,與對照組比較,奧氮平組大鼠的體質量、血糖、血脂均顯著升高,差異有統計學意義(P＜0.05);奧氮平組大鼠的血清TNF-α、IL-6和CRP分彆為(1.57±0.04) ng/ml、(127.47± 11.38) pg/ml和(2.68±0.06) mg/ml,與對照組比較[(0.59±0.03)ng/ml、(96.58±8.77) pg/ml和(1.86±0.04) mg/ml]有不同程度的升高,差異有統計學意義(P＜0.05).實驗組大鼠電擊次數和逃避潛伏期均高于對照組(P＜0.05).FBS分彆與hs-CRP、IL-6和TNF-α呈正相關(r=0.385,0.260,1.280;均P＜0.05).結論 奧氮平可以導緻大鼠糖脂代謝紊亂,血清TNF-α,IL-6和CRP分泌增加,TNF-α、IL-6和CRP濃度變化與FBS水平呈正相關.血糖升高可促進細胞因子細胞毒性作用,導緻大鼠認知功能損害.
목적 관찰오담평유도비반대서당지대사문란대지방원성세포인자영향화인지공능손상실험연구.방법 40지대서수궤분위대조조화실험조,매조20지.대조조대서진행보통사료위양,실험조재보통사량위양기출상행오담평(1.2 mg·kg-1)관위4주건립오담평유도비반대서모형.채용매련면역측정법측정2조대서혈청중종류배사인자α(TNF-α),백세포개소6(IL-6)화C-반응단백(CRP)적함량,생화비색법측정혈청포도당(FBS)함량.채용Y-형미궁관찰2조대서학습、기억능력화도피잠복기변화.결과 관위4주후,여대조조비교,오담평조대서적체질량、혈당、혈지균현저승고,차이유통계학의의(P＜0.05);오담평조대서적혈청TNF-α、IL-6화CRP분별위(1.57±0.04) ng/ml、(127.47± 11.38) pg/ml화(2.68±0.06) mg/ml,여대조조비교[(0.59±0.03)ng/ml、(96.58±8.77) pg/ml화(1.86±0.04) mg/ml]유불동정도적승고,차이유통계학의의(P＜0.05).실험조대서전격차수화도피잠복기균고우대조조(P＜0.05).FBS분별여hs-CRP、IL-6화TNF-α정정상관(r=0.385,0.260,1.280;균P＜0.05).결론 오담평가이도치대서당지대사문란,혈청TNF-α,IL-6화CRP분비증가,TNF-α、IL-6화CRP농도변화여FBS수평정정상관.혈당승고가촉진세포인자세포독성작용,도치대서인지공능손해.
Objective To observe the adipose-derived cytokine changes and aggravate cognitive impairment in olanzapine-induced obese rats caused by glucose metabolic disorder.Methods 20 rats fed with ordinary fodder were used as normal control group,olanzapine group of 20 rats fed with olanzapine(1.2 mg · kg-1) and ordinary fodder for 4 weeks.Successfully established experimental model rats induced by olanzapine after 4 weeks.Serum tumor necrosis factor α(TNF-α),interleukins 6 (IL-6) and C-reactive protein (CRP) contents were measured by Elisa.Serum glucose contents were determined by biochemical colorimetric method and blood lipid contents determined with automatic biochemical analyzer.Learning,memory capacity and escape latency were detected with Maze test.Results After administration 4 weeks,the levels of body weight,blood glucose and blood lipid in olanzapine group were higher than those in control group.The serum TNF-α((1.57±0.04) ng/ml),IL-6((127.47±11.38) pg/ml) and CRP ((2.68±0.06) mg/ml) in olanzapine group rised,compared with control group ((0.59±0.03) ng/ml,(96.58± 8.77) pg/ml and (1.86±0.04) mg/ml respectively),the differences were statistically significant(P＜0.05).Electric shocks and escape latency in olanzapine group were higher than those in control group (P＜0.05).The FBS had positive correlation with hs-CRP,IL-6 and TNF-α respectively (r=0.385,0.260,1.280; all P＜0.05).Conclusion Olanzapine can induce metabolic disturbance of blood glucose,blood hpid,and the increase of serum TNF-α,IL-6 and CRP levels in rats.Positive correlation is showed between TNF-of and FBS.Hyperglycemia can promote cell toxicity and leads to cognitive dysfunction in rats.
