中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
8期
715-719
,共5页
林娜%栾晓%祝海%逄明杰%郭菲菲%孙向荣%公衍玲%徐珞
林娜%欒曉%祝海%逄明傑%郭菲菲%孫嚮榮%公衍玲%徐珞
림나%란효%축해%방명걸%곽비비%손향영%공연령%서락
Nesfatin-1%饮水行为%血管紧张素Ⅱ%大鼠
Nesfatin-1%飲水行為%血管緊張素Ⅱ%大鼠
Nesfatin-1%음수행위%혈관긴장소Ⅱ%대서
Nesfatin-1%Drinking behaviour%Angiotensin Ⅱ%Rat
目的 观察nesfatin-1对大鼠饮水的影响,探讨Nesfatin-1对下丘脑调节水和电解质平衡的作用机制.方法 采用侧脑室埋管、注射药物和分子生物学等方法,观察内外源性Nesfatin-1对药物致渴、生理性致渴大鼠饮水的影响,并观察禁水对大鼠NUCB2/nesfatin-1基因表达的影响.结果 侧脑室注射外源性150、450 pmol nesfatin-1,大鼠0～ 120 min内各时间段的饮水量显著减少[150 pmol:(1.3±0.2)～(1.85±0.3) ml/100g;450 pmol:(0.8±0.4)～ (1.2±0.4) ml/100g vs NS:(2.5±0.3)～(3.1 ±0.2) ml/100g],且呈显著剂量依赖关系(P＜0.05～ 0.01),但2～24 h饮水量无显著改变(P＞0.05).侧脑室注射nesfatin-1,10 min后注射致渴剂血管紧张素Ⅱ,注射150 pmol nesfatin-1的大鼠在15～30 min饮水量显著减少[150 pmol:(2.17±0.6) ml/100g vs NS(3.62± 1.0) ml/100g,P＜0.05],注射450 pmol nesfatin-1大鼠在注药后120 min内各时间段的饮水量均显著减少[450 pmol(0.31±0.1)～(2.87±0.8) ml/100g vs NS(2.31±0.7)～(7.05±0.2) ml/100g],呈剂量依赖关系(P＜0.05～0.01),但5～24 h饮水量无显著改变(P＞0.05).皮下注射高渗盐水,大鼠饮水量急剧增多,侧脑室注射150或450 pmol nesfatin-1,大鼠60 min内各时间段的饮水量均显著减少[150pmo1(0.25±0.2)～(1.6±0.25) ml/100g;450 pmol(0.1±0.15)～(1.92±0.2) ml/100g vs NS(0.9±0.2)～(2.9±0.2) ml/100g,P＜0.05～0.01],但恢复供水后60～120 min或2～24 h的饮水量无显著差异(P＞0.05).下丘脑PVN注射反义nesfatin-1 RNA,第2次注射后3h,侧脑室注射血管紧张素Ⅱ (50 pmol/2μl),大鼠饮水量显著增加[反义nesfatin-1:(1.87±0.4)～ (2.77±0.6) ml/100g vs NS(0.72±0.1)～(1.17±0.4) ml/100g,P＜0.01],但大鼠体质量和摄食量差异无统计学意义(P＞0.05).大鼠禁水18h,下丘脑内NUCB2基因表达显著降低[禁水组:(-7.2±1.1)％vs自由饮水组:(0.82±0.4)％,P＜0.01];而禁水后再给水的大鼠,下丘脑NUCB2基因表达显著升高[禁水后给水组:(8.1±1.1)％ vs自由饮水组:(0.82±0.4)％,P＜0.01].结论 Nesfatin-1参与大鼠生理和药理性致渴调控,nesfatin-1对大鼠饮水调控是下丘脑调节水和电解质平衡的重要环节.
目的 觀察nesfatin-1對大鼠飲水的影響,探討Nesfatin-1對下丘腦調節水和電解質平衡的作用機製.方法 採用側腦室埋管、註射藥物和分子生物學等方法,觀察內外源性Nesfatin-1對藥物緻渴、生理性緻渴大鼠飲水的影響,併觀察禁水對大鼠NUCB2/nesfatin-1基因錶達的影響.結果 側腦室註射外源性150、450 pmol nesfatin-1,大鼠0～ 120 min內各時間段的飲水量顯著減少[150 pmol:(1.3±0.2)～(1.85±0.3) ml/100g;450 pmol:(0.8±0.4)～ (1.2±0.4) ml/100g vs NS:(2.5±0.3)～(3.1 ±0.2) ml/100g],且呈顯著劑量依賴關繫(P＜0.05～ 0.01),但2～24 h飲水量無顯著改變(P＞0.05).側腦室註射nesfatin-1,10 min後註射緻渴劑血管緊張素Ⅱ,註射150 pmol nesfatin-1的大鼠在15～30 min飲水量顯著減少[150 pmol:(2.17±0.6) ml/100g vs NS(3.62± 1.0) ml/100g,P＜0.05],註射450 pmol nesfatin-1大鼠在註藥後120 min內各時間段的飲水量均顯著減少[450 pmol(0.31±0.1)～(2.87±0.8) ml/100g vs NS(2.31±0.7)～(7.05±0.2) ml/100g],呈劑量依賴關繫(P＜0.05～0.01),但5～24 h飲水量無顯著改變(P＞0.05).皮下註射高滲鹽水,大鼠飲水量急劇增多,側腦室註射150或450 pmol nesfatin-1,大鼠60 min內各時間段的飲水量均顯著減少[150pmo1(0.25±0.2)～(1.6±0.25) ml/100g;450 pmol(0.1±0.15)～(1.92±0.2) ml/100g vs NS(0.9±0.2)～(2.9±0.2) ml/100g,P＜0.05～0.01],但恢複供水後60～120 min或2～24 h的飲水量無顯著差異(P＞0.05).下丘腦PVN註射反義nesfatin-1 RNA,第2次註射後3h,側腦室註射血管緊張素Ⅱ (50 pmol/2μl),大鼠飲水量顯著增加[反義nesfatin-1:(1.87±0.4)～ (2.77±0.6) ml/100g vs NS(0.72±0.1)～(1.17±0.4) ml/100g,P＜0.01],但大鼠體質量和攝食量差異無統計學意義(P＞0.05).大鼠禁水18h,下丘腦內NUCB2基因錶達顯著降低[禁水組:(-7.2±1.1)％vs自由飲水組:(0.82±0.4)％,P＜0.01];而禁水後再給水的大鼠,下丘腦NUCB2基因錶達顯著升高[禁水後給水組:(8.1±1.1)％ vs自由飲水組:(0.82±0.4)％,P＜0.01].結論 Nesfatin-1參與大鼠生理和藥理性緻渴調控,nesfatin-1對大鼠飲水調控是下丘腦調節水和電解質平衡的重要環節.
목적 관찰nesfatin-1대대서음수적영향,탐토Nesfatin-1대하구뇌조절수화전해질평형적작용궤제.방법 채용측뇌실매관、주사약물화분자생물학등방법,관찰내외원성Nesfatin-1대약물치갈、생이성치갈대서음수적영향,병관찰금수대대서NUCB2/nesfatin-1기인표체적영향.결과 측뇌실주사외원성150、450 pmol nesfatin-1,대서0～ 120 min내각시간단적음수량현저감소[150 pmol:(1.3±0.2)～(1.85±0.3) ml/100g;450 pmol:(0.8±0.4)～ (1.2±0.4) ml/100g vs NS:(2.5±0.3)～(3.1 ±0.2) ml/100g],차정현저제량의뢰관계(P＜0.05～ 0.01),단2～24 h음수량무현저개변(P＞0.05).측뇌실주사nesfatin-1,10 min후주사치갈제혈관긴장소Ⅱ,주사150 pmol nesfatin-1적대서재15～30 min음수량현저감소[150 pmol:(2.17±0.6) ml/100g vs NS(3.62± 1.0) ml/100g,P＜0.05],주사450 pmol nesfatin-1대서재주약후120 min내각시간단적음수량균현저감소[450 pmol(0.31±0.1)～(2.87±0.8) ml/100g vs NS(2.31±0.7)～(7.05±0.2) ml/100g],정제량의뢰관계(P＜0.05～0.01),단5～24 h음수량무현저개변(P＞0.05).피하주사고삼염수,대서음수량급극증다,측뇌실주사150혹450 pmol nesfatin-1,대서60 min내각시간단적음수량균현저감소[150pmo1(0.25±0.2)～(1.6±0.25) ml/100g;450 pmol(0.1±0.15)～(1.92±0.2) ml/100g vs NS(0.9±0.2)～(2.9±0.2) ml/100g,P＜0.05～0.01],단회복공수후60～120 min혹2～24 h적음수량무현저차이(P＞0.05).하구뇌PVN주사반의nesfatin-1 RNA,제2차주사후3h,측뇌실주사혈관긴장소Ⅱ (50 pmol/2μl),대서음수량현저증가[반의nesfatin-1:(1.87±0.4)～ (2.77±0.6) ml/100g vs NS(0.72±0.1)～(1.17±0.4) ml/100g,P＜0.01],단대서체질량화섭식량차이무통계학의의(P＞0.05).대서금수18h,하구뇌내NUCB2기인표체현저강저[금수조:(-7.2±1.1)％vs자유음수조:(0.82±0.4)％,P＜0.01];이금수후재급수적대서,하구뇌NUCB2기인표체현저승고[금수후급수조:(8.1±1.1)％ vs자유음수조:(0.82±0.4)％,P＜0.01].결론 Nesfatin-1삼여대서생리화약이성치갈조공,nesfatin-1대대서음수조공시하구뇌조절수화전해질평형적중요배절.
Objective To explore the effect of nesfatin-1 on water intake and the mechanism of nesfatin-1 in regulating water and electrolyte balance in hypothalamus of rats.Methods Lateral ventricle implantation tube,drugs and molecular biology were used to observe the effects of exogenous and endogenous nesfatin-1 on water intake in drug causing thirsty rats and on physiological thirsty rats.Meanwhile the gene expression of NUCB2/Nesfatin-1 of water ban rats has been observed.Results Lateral ventricle injection of 150,450 pmol exogenous nesfatin-1,the water intake in 0-120 min of rats significantly reduced (150 pmol(1.3±0.2)-(1.85±0.3) ml/100g;450 pmol(0.8±0.4)-(1.2±0.4) ml/100g vs NS(2.5±0.3)-(3.1±0.2)ml/100g) and showed a significant dose-response relationship (P＜0.05-0.01),but the water intake in 2-24 h had no significant change (P＞0.05).Angiotensin Ⅱ were administrated to lateral ventricle 10 min after injection of nesfatin-1.After injection of 150 pmol nesfatin-1,water intake in 15-30 min of rats significantly reduced (150 pmol(2.17±0.6) ml/100g vs NS(3.62 ± 1.0)ml/100g,P＜0.05).After injection of 450 pmol nesfatin-1,water intake in each time period within 120 rain of rats reduced significantly (450pmol (0.31 ± 0.1)-(2.87 ± 0.8) ml/100g vs NS (2.31 ± 0.7)-(7.05 ± 0.2) ml/100g) and presented a dose-response manner (P＜0.05-0.01),but 5-24 h water intake had no significant change (P＞0.05).Subcutaneous injection of hypertonic saline,and water intake in rats increase rapidly(The water intake of rats with subcutaneous injection of hypertonic saline increase rapidly,while the water intake of rats with 150 pmol nesfatin-1 injection in lateral ventricle within 15～ 30 min and the water intake of rats with 450 pmol nesfatin-1 injection in lateral ventricle within 120 min significantly reduced.Lateral ventricle injection of 150 or 450 pmol nesfatin-1,water intake of each time period within 60 min in rats significantly reduced (150 pmol:(0.25±0.2)-(1.6±0.25) ml/100g ;450 pmol(0.1±0.15)-(1.92±0.2) ml/100g vs NS(0.9±0.2)-(2.9±0.2) ml/100g,P＜0.05 ～0.01),but after restore water supply 60～ 120 min or 2-24 h,the water intake had no significant difference (P＞0.05).Three hours After the second time hypothalamus PVN injection of antisense RNA nesfatin-1,angiotensin Ⅱ (50 pmol/2 mul) were given and water intake in rats increased significantly (Antisense nesfatin-1 (1.87±0.4)-(2.77±0.6)ml/100g vs NS(0.72±0.1)-(1.17±0.4) ml/100g,P＜0.01),but the body weight and food intake in rats had no significant difference (P＞0.05).NUCB2 gene expression was significantly reduced in the hypothalamus (water ban:(-7.2± 1.1)％ vs free drinking water:(0.82±0.4)％,P＜0.01) in rats after 18 hours water ban;The hypothalamus NUCB2 gene expression had a significant rise (water supply restore:(8.1± 1.1) ％ vs free drinking water:(0.82± 0.4) ％,P ＜0.01) in rats with water supply restore.Conclusion Nesfatin-1 involves in the regulation of water intake in physiology or drug causing thirsty.Nesfatin-1 plays an important role in the hypothalamus to regulate water-electrolyte balance and thirsty mechanisms.
