CAJ | 학술논문

目的通过分析孕鼠感染多聚肌苷酸:多聚胞苷酸(Polyriboinosinic-polyribocytidilic acid,Poly (I∶C)后子代行为学和脑白质髓鞘碱性蛋白(myelin basic protein,MBP)的变化,了解脑白质在孕期感染所致子代行为异常中的作用.方法两组模型组孕鼠分别给予5 mg/kg Poly(I∶C)和10 mg/kgPoly(I∶C)处理,对照组给予5 mg/kg生理盐水处理.待子代生长至8周时用前脉冲抑制实验、被动规避实验、主动规避实验检测子代精神分裂症样行为,用免疫组化方法观察脑白质MBP的变化.结果前脉冲抑制结果显示PP2、PP4、PP8差异有显著性(F=10.381,P=0.001,F=10.313,P=0.001,F=15.233,P=0.000),两个模型组均小于对照组,双倍剂量组均小于单倍剂量组(P＜0.05);被动规避结果显示T1、T2差异有显著性(F=17.524,P=0.000,F=23.555,P=0.000),两个模型组的T1时间均大于对照组,双倍剂量组大于单倍剂量组(P＜0.05);两模型组T2时间均小于对照组,双倍剂量组小于单倍剂量组(P＜0.05);主动规避结果显示总条件反射次数和条件反射率差异有显著性(F=8.631,P=0.000,F=6.986,P=0.001),两个模型组均小于对照组,双倍剂量组小于单倍剂量组(P＜0.05);两模型组大鼠脑白质MBP免疫组化结果与对照组相比有统计学意义(P＜0.05),两模型组比较差异无统计学意义(P＞0.05).结论 Poly(I∶C)处理后子鼠存在行为学和MBP的改变,且行为学改变的程度与药物剂量有相关性.
목적 통과분석잉서감염다취기감산:다취포감산(Polyriboinosinic-polyribocytidilic acid,Poly (I∶C)후자대행위학화뇌백질수초감성단백(myelin basic protein,MBP)적변화,료해뇌백질재잉기감염소치자대행위이상중적작용.방법량조모형조잉서분별급여5 mg/kg Poly(I∶C)화10 mg/kgPoly(I∶C)처리,대조조급여5 mg/kg생리염수처리.대자대생장지8주시용전맥충억제실험、피동규피실험、주동규피실험검측자대정신분렬증양행위,용면역조화방법관찰뇌백질MBP적변화.결과 전맥충억제결과현시PP2、PP4、PP8차이유현저성(F=10.381,P=0.001,F=10.313,P=0.001,F=15.233,P=0.000),량개모형조균소우대조조,쌍배제량조균소우단배제량조(P＜0.05);피동규피결과현시T1、T2차이유현저성(F=17.524,P=0.000,F=23.555,P=0.000),량개모형조적T1시간균대우대조조,쌍배제량조대우단배제량조(P＜0.05);량모형조T2시간균소우대조조,쌍배제량조소우단배제량조(P＜0.05);주동규피결과현시총조건반사차수화조건반사솔차이유현저성(F=8.631,P=0.000,F=6.986,P=0.001),량개모형조균소우대조조,쌍배제량조소우단배제량조(P＜0.05);량모형조대서뇌백질MBP면역조화결과여대조조상비유통계학의의(P＜0.05),량모형조비교차이무통계학의의(P＞0.05).결론 Poly(I∶C)처리후자서존재행위학화MBP적개변,차행위학개변적정도여약물제량유상관성.
Objective By analyzing the changes in behavior and the myelin basic protein (MBP) of the offspring in adult that treated with Poly(I∶C) during pregnancy,and to understand the role of white matter abnormalities in the abnormal behavior of the offspring induced by infection in maternal hosts.Methods Two models maternal female rats were given Poly(I∶ C) with 5 mg/kg and 10mg/kg respectively during the early pregnancy,and control maternal female rats was administered 5 mg/kg saline.The prepulse inhibition test,passive avoidance test and active avoidance test were used to evaluate schizophrenia like behaviors for each groups offspring in 8 weeks,and the expression of MBP was detected by immunohistochemical staining methods.Results The results of prepulse inhibition test showed that significant differences of PP2,PP4 and PP8 results existed among control group,single-dose model group and double-dose model group (F=10.381,P=0.001,F=10.313,P=0.001,F=15.233,P=0.000).Compared with the control group,the two model groups showed significantly lower,the double-dose model group was lower than single-dose model group (P＜0.05).In passive avoidance test,there were significant differences of T1 and T2 results existed among control group,single-dose model group and double-dose model group (F=23.555,P=0.000,F=17.524,P=0.000).The T1 results of two model groups were significantly higher than control group,the double-dose model group was significantly higher than single-dose model group (P＜0.05) ; the T2 results of two model groups were lower than control group,the double-dose model group was lower than single-dose model group(P＜0.05).The results of passive avoidance test indicated that significant differences existed among control group,single-dose model group and double-dose model group in whole period of testing and total conditioned response rate(F=8.631,P=0.000,F=6.986,P=0.001),the two model groups were significantly lower than control group,double-dose model group was significantly lower than single-dose model group (P＜0.05).MBP results of two model groups were significantly lower than control group,two model groups had no significant difference (P＞ 0.05).Conclusion The adult offspring that were treated with Poly (I∶C) exit abnormal behavior and damaged white matter,and there is a correlation between the degree of abnormal behavior and drug dose.