CAJ | 학술논문

目的观察宁心解郁胶囊对慢性抑郁大鼠大脑皮质单胺类神经递质及其代谢产物的影响.方法以慢性不可预见性的刺激配合孤养,制备慢性综合应激法抑郁大鼠模型,造模成功的大鼠按随机数字表分为模型组、宁心解郁胶囊高剂量组[10.8mg/(kg·d)]、宁心解郁胶囊中剂量组[3.6 mg/(kg·d)]、宁心解郁胶囊低剂量组[1.2 mg/(kg·d)]、盐酸帕罗西汀组[3.6 mg/(kg·d)].采用HPLC-ECD系统测定各个实验组大鼠大脑皮质中单胺类神经递质,去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)及其主要代谢产物3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)的表达含量.结果与空白组相比,模型组NE、DA、5-HT含量显著下降,差异具有统计学意义(P＜0.05或P＜0.01).与模型组相比,宁心解郁各剂量组的DA含量均升高;低剂量和高剂量组的5-HT含量升高[分别为(272.15± 129.89) ng/g,(445.08± 127.56)ng/g,(375.33±52.38) ng/g];低剂量组和中剂量组的NE含量升高[(408.08±89.55) ng/g,(530.12± 149.87)ng/g,(542.53±96.10) ng/g],中剂量和高剂量组的HVA含量升高,低剂量组的DOPAC含量升高;盐酸帕罗西汀组的5-HT、DA、NE含量升高,差异有统计学意义(P＜0.01或P＜0.05).结论宁心解郁胶囊具有抗抑郁作用,其抗抑郁的作用机制与可调节大脑皮层的单胺类神经递质的含量及其代谢产物有关.
목적 관찰저심해욱효낭대만성억욱대서대뇌피질단알류신경체질급기대사산물적영향.방법 이만성불가예견성적자격배합고양,제비만성종합응격법억욱대서모형,조모성공적대서안수궤수자표분위모형조、저심해욱효낭고제량조[10.8mg/(kg·d)]、저심해욱효낭중제량조[3.6 mg/(kg·d)]、저심해욱효낭저제량조[1.2 mg/(kg·d)]、염산파라서정조[3.6 mg/(kg·d)].채용HPLC-ECD계통측정각개실험조대서대뇌피질중단알류신경체질,거갑신상선소(NE)、다파알(DA)、5-간색알(5-HT)급기주요대사산물3,4-이간기분을산(DOPAC)、고향초산(HVA)적표체함량.결과 여공백조상비,모형조NE、DA、5-HT함량현저하강,차이구유통계학의의(P＜0.05혹P＜0.01).여모형조상비,저심해욱각제량조적DA함량균승고;저제량화고제량조적5-HT함량승고[분별위(272.15± 129.89) ng/g,(445.08± 127.56)ng/g,(375.33±52.38) ng/g];저제량조화중제량조적NE함량승고[(408.08±89.55) ng/g,(530.12± 149.87)ng/g,(542.53±96.10) ng/g],중제량화고제량조적HVA함량승고,저제량조적DOPAC함량승고;염산파라서정조적5-HT、DA、NE함량승고,차이유통계학의의(P＜0.01혹P＜0.05).결론 저심해욱효낭구유항억욱작용,기항억욱적작용궤제여가조절대뇌피층적단알류신경체질적함량급기대사산물유관.
Objective To observe the impact of ningxinjieyu capsule on contents of monoamine neurotransmitters and their metabolites in cerebral cortex of chronic depression model rats.Methods The chronic stressed depression model rats were established by chronic unpredictable stress and separation.the model rats randomly divided into model group,ningxinjieyu high-dose group(10.8 mg/(kg · d)),mid-dose group(3.6 mg/(kg · d)),low-dose group(1.2 mg/(kg · d)) and Fluoxetine group(3.6 mg/(kg · d)) and the normal rats as the control group.Finally the changes of Noradrenaline (NE),Dopamine (DA),5-hydroxy tryptamine (5-HT),3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) would be observed using HPLC-ECD technique.Results Compared with the control group,the contents of NE,DA and 5-HT were significantly lower in the model group (P＜0.01).Compared with the model group,the contents of DA were increased in the different concentrations of ningxinjieyue capsule groups,and the contents of 5-HT were higher in the low-dose and high-dose groups ((272.15± 129.89) ng/g,(445.08± 127.56) ng/g,(375.33±52.38) ng/g) ; the contents of NE were increased in the low-dose and mid-dose groups ((408.08 ± 89.55) ng/g,(530.12± 149.87) ng/g,(542.53 ± 96.10) ng/g) ; the contents of HVA were increased in the mid-dose and high-dose groups; the contents of DOPAC were increased in the low-dose group; the contents of 5-HT,DA and NE were increased in the fluoxetine group(P＜0.01 or P＜0.05).Conclusion Ningxinjieyu capsule has antidepressant effect,the mechanisms might be regulated to the contents of monoamine neurotransmitters and their metabolites in cerebral cortex.