中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
10期
872-874
,共3页
管强%李云霞%靳令经%聂志余
管彊%李雲霞%靳令經%聶誌餘
관강%리운하%근령경%섭지여
磁共振波谱%马来酸桂哌齐特%脑缺血
磁共振波譜%馬來痠桂哌齊特%腦缺血
자공진파보%마래산계고제특%뇌결혈
Magnetic resonance spectroscopy%Cinepazide maleate%Cerebral ischemia
目的 动态监测马来酸桂哌齐特(CM)对脑组织缺血区域的影响,明确CM对于急性脑缺血大鼠模型的神经保护功能及相关作用机制.方法 ①SD雄性大鼠,采用线栓法制作大鼠缺血再灌注模型,分为缺血再灌注组、马来酸桂哌齐特(CM)治疗组,每组8只大鼠.再灌注后对CM治疗组立即从尾静脉注射CM.术前与再灌注后6h、24 h、3d进行常规MRI检测及氢质子磁共振波谱(1HMRS)分析.结果 ①常规MRI检测:各组大鼠再灌注后6h、24h、3dT2加权像(T2WI)上梗死面积无明显差异(P＞0.05).②1 HMRS分析:缺血再灌注组术前、术后6h、24h、3d氮-乙酰天门冬氨酸(NAA)/磷酸肌酸和肌酸(PCr+Cr)值分别为(1.53±0.20)、(0.50±0.17)、(0.44±0.13)、(0.40±0.10),乳酸(Lac)/(PCr+Cr)值分别为(0.03±0.01)、(1.10±0.28)、(1.30±0.23)、(1.23±0.19).CM治疗组术前、术后6h、24 h、3 d NAA/(PCr+Cr)值分别为(1.44±0.22)、(0.68±0.13)、(0.61±0.10)、(0.53±0.09),Lac/(PCr+Cr)值分别为0.02±0.01、0.85±0.25、0.99±0.20、0.90±0.15.与术前相比,缺血再灌注组NAA/(PCr+Cr)值在术后6h、24 h、3d明显降低(P＜0.01),Lac/(PCr+Cr)值明显增高(P＜0.01).与缺血再灌注组相比,CM治疗组NAA/(PCr+Cr)值在再灌注后6h、24 h、3d均显著增高(P＜0.05),Lac/(PCr+Cr)值均显著降低(P＜0.05).结论 脑缺血再灌注后,早期应用CM可以改善脑缺血再灌注区的缺血缺氧环境,减少神经元的坏死.
目的 動態鑑測馬來痠桂哌齊特(CM)對腦組織缺血區域的影響,明確CM對于急性腦缺血大鼠模型的神經保護功能及相關作用機製.方法 ①SD雄性大鼠,採用線栓法製作大鼠缺血再灌註模型,分為缺血再灌註組、馬來痠桂哌齊特(CM)治療組,每組8隻大鼠.再灌註後對CM治療組立即從尾靜脈註射CM.術前與再灌註後6h、24 h、3d進行常規MRI檢測及氫質子磁共振波譜(1HMRS)分析.結果 ①常規MRI檢測:各組大鼠再灌註後6h、24h、3dT2加權像(T2WI)上梗死麵積無明顯差異(P＞0.05).②1 HMRS分析:缺血再灌註組術前、術後6h、24h、3d氮-乙酰天門鼕氨痠(NAA)/燐痠肌痠和肌痠(PCr+Cr)值分彆為(1.53±0.20)、(0.50±0.17)、(0.44±0.13)、(0.40±0.10),乳痠(Lac)/(PCr+Cr)值分彆為(0.03±0.01)、(1.10±0.28)、(1.30±0.23)、(1.23±0.19).CM治療組術前、術後6h、24 h、3 d NAA/(PCr+Cr)值分彆為(1.44±0.22)、(0.68±0.13)、(0.61±0.10)、(0.53±0.09),Lac/(PCr+Cr)值分彆為0.02±0.01、0.85±0.25、0.99±0.20、0.90±0.15.與術前相比,缺血再灌註組NAA/(PCr+Cr)值在術後6h、24 h、3d明顯降低(P＜0.01),Lac/(PCr+Cr)值明顯增高(P＜0.01).與缺血再灌註組相比,CM治療組NAA/(PCr+Cr)值在再灌註後6h、24 h、3d均顯著增高(P＜0.05),Lac/(PCr+Cr)值均顯著降低(P＜0.05).結論 腦缺血再灌註後,早期應用CM可以改善腦缺血再灌註區的缺血缺氧環境,減少神經元的壞死.
목적 동태감측마래산계고제특(CM)대뇌조직결혈구역적영향,명학CM대우급성뇌결혈대서모형적신경보호공능급상관작용궤제.방법 ①SD웅성대서,채용선전법제작대서결혈재관주모형,분위결혈재관주조、마래산계고제특(CM)치료조,매조8지대서.재관주후대CM치료조립즉종미정맥주사CM.술전여재관주후6h、24 h、3d진행상규MRI검측급경질자자공진파보(1HMRS)분석.결과 ①상규MRI검측:각조대서재관주후6h、24h、3dT2가권상(T2WI)상경사면적무명현차이(P＞0.05).②1 HMRS분석:결혈재관주조술전、술후6h、24h、3d담-을선천문동안산(NAA)/린산기산화기산(PCr+Cr)치분별위(1.53±0.20)、(0.50±0.17)、(0.44±0.13)、(0.40±0.10),유산(Lac)/(PCr+Cr)치분별위(0.03±0.01)、(1.10±0.28)、(1.30±0.23)、(1.23±0.19).CM치료조술전、술후6h、24 h、3 d NAA/(PCr+Cr)치분별위(1.44±0.22)、(0.68±0.13)、(0.61±0.10)、(0.53±0.09),Lac/(PCr+Cr)치분별위0.02±0.01、0.85±0.25、0.99±0.20、0.90±0.15.여술전상비,결혈재관주조NAA/(PCr+Cr)치재술후6h、24 h、3d명현강저(P＜0.01),Lac/(PCr+Cr)치명현증고(P＜0.01).여결혈재관주조상비,CM치료조NAA/(PCr+Cr)치재재관주후6h、24 h、3d균현저증고(P＜0.05),Lac/(PCr+Cr)치균현저강저(P＜0.05).결론 뇌결혈재관주후,조기응용CM가이개선뇌결혈재관주구적결혈결양배경,감소신경원적배사.
Objective To dynamically monitor the effect of Cinepazide maleate (CM) on ischemic region of the brain and to elucidate the neuroprotection of CM on acute cerebral ischemic rat model and related mechanisms.Methods The rat model of acute brain ischemia-reperfusion was established with tighting threads.They were randomly divided into ischemia-reperfusion group and CM treatment group(n=8 in each group).Rats in CM treatment group were given 3 mg / kg CM by caudal intravenous injection immediately after brain ischemia-reperfusion.T2 weighted imaging(T2WI) and proton magnetic resonance spectroscopy (1HMRS) were performed before operation and at 6 hours,24 hours and 3 days after reperfusion.Results At 6 h,24 h and 3 d after operation there was no difference for areas of cerebral ischemia on T2WI between 2 groups.The values of N-acetylaspartate(NAA) (Phosphocreatine(PCr) +Creartine(Cr)) in ischemia-reperfusion group rats were (1.53±0.20),(0.50 ±0.17),(0.44±0.13),(0.40±0.10) before operation and at 6 h,24 h,3 d after operation,respectively.And in CM treatment group the values of NAA/(PCr+Cr) were (1.44±0.22),(0.68±0.13),(0.61±0.10),(0.53±0.09) at the same time points.The values of lactate(Lac)/(PCr+Cr) in ischemia-reperfusion group were (0.03±0.01),(1.10 ±0.28),(1.30± 0.23),(1.23± 0.19) before operation and at 6 h,24 h,3 d after operation,respectively.And in CM treatment group the values of Lac/(PCr+Cr) were (0.02±0.01),(0.85±0.25),(0.99±0.20),(0.90±0.15) at the same time points.The rats in ischemia-reperfusion group had lower value of NAA/(PCr+Cr) (P＜0.01) and higher value of Lac/(PCr+Cr) at all time points after operation than those before operation.Compared with rats in ischemia-reperfusion group,the value of NAA/(PCr+Cr) increased(P＜0.05) and the value of Lac/(PCr+Cr) decreased(P＜0.05) decreased significantly for CM treatment group rats.Conclusion CM treatment can decrease the intracellular accumulation of lactic acid and reduce neuronal necrosis in acute brain ischemia-reperfusion rats.
