中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
10期
875-877
,共3页
Hes1%RBP-Jk%碱性成纤维细胞生长因子%辐射损伤
Hes1%RBP-Jk%堿性成纖維細胞生長因子%輻射損傷
Hes1%RBP-Jk%감성성섬유세포생장인자%복사손상
Hes1 protein%RBP-Jk protein%Basic fibroblast growth factor%Radiation injury
目的 观察外源性碱性成纤维细胞生长因子(bFGF)对辐射诱导的c17.2神经干细胞凋亡的影响,探讨Nocth信号通路下游蛋白Hes1,RBP-Jk表达与bFGF之间的关系.方法 MTT法检测细胞的活性;培养贴壁后的细胞利用直线加速器进行照射,5 min后分别加入0 ng/ml、20 ng/ml、40 ng/ml、80 ng/ml的bFGF,培养48 h后提取各组总蛋白,利用Western blot检测Hes1、RBP-JK蛋白含量的改变.结果 细胞照射后,照射组与对照组相比,细胞的生长受到明显的抑制,加入0 ng/ml bFGF组的OD值为(0.61±0.81), 80 ng/ml bFGF组的OD值为(1.21±1.01),对照组的OD值为(1.51±1.13),各照射组与对照组相比差异有统计学意义(P＜0.05),各照射组之间两两比较差异均有统计学意义(P＜0.05).Western blot结果显示照射后的细胞随着bFGF浓度的增高,RBP-JK表达量逐渐降低,而Hes1的表达含量逐渐升高.结论 外源性bFGF对放射诱导的C17.2神经干细胞凋亡有抑制作用,bFGF调节Notch信号通路下游蛋白Hes1、RBP-JK的表达,抑制放射后神经干细胞的凋亡.
目的 觀察外源性堿性成纖維細胞生長因子(bFGF)對輻射誘導的c17.2神經榦細胞凋亡的影響,探討Nocth信號通路下遊蛋白Hes1,RBP-Jk錶達與bFGF之間的關繫.方法 MTT法檢測細胞的活性;培養貼壁後的細胞利用直線加速器進行照射,5 min後分彆加入0 ng/ml、20 ng/ml、40 ng/ml、80 ng/ml的bFGF,培養48 h後提取各組總蛋白,利用Western blot檢測Hes1、RBP-JK蛋白含量的改變.結果 細胞照射後,照射組與對照組相比,細胞的生長受到明顯的抑製,加入0 ng/ml bFGF組的OD值為(0.61±0.81), 80 ng/ml bFGF組的OD值為(1.21±1.01),對照組的OD值為(1.51±1.13),各照射組與對照組相比差異有統計學意義(P＜0.05),各照射組之間兩兩比較差異均有統計學意義(P＜0.05).Western blot結果顯示照射後的細胞隨著bFGF濃度的增高,RBP-JK錶達量逐漸降低,而Hes1的錶達含量逐漸升高.結論 外源性bFGF對放射誘導的C17.2神經榦細胞凋亡有抑製作用,bFGF調節Notch信號通路下遊蛋白Hes1、RBP-JK的錶達,抑製放射後神經榦細胞的凋亡.
목적 관찰외원성감성성섬유세포생장인자(bFGF)대복사유도적c17.2신경간세포조망적영향,탐토Nocth신호통로하유단백Hes1,RBP-Jk표체여bFGF지간적관계.방법 MTT법검측세포적활성;배양첩벽후적세포이용직선가속기진행조사,5 min후분별가입0 ng/ml、20 ng/ml、40 ng/ml、80 ng/ml적bFGF,배양48 h후제취각조총단백,이용Western blot검측Hes1、RBP-JK단백함량적개변.결과 세포조사후,조사조여대조조상비,세포적생장수도명현적억제,가입0 ng/ml bFGF조적OD치위(0.61±0.81), 80 ng/ml bFGF조적OD치위(1.21±1.01),대조조적OD치위(1.51±1.13),각조사조여대조조상비차이유통계학의의(P＜0.05),각조사조지간량량비교차이균유통계학의의(P＜0.05).Western blot결과현시조사후적세포수착bFGF농도적증고,RBP-JK표체량축점강저,이Hes1적표체함량축점승고.결론 외원성bFGF대방사유도적C17.2신경간세포조망유억제작용,bFGF조절Notch신호통로하유단백Hes1、RBP-JK적표체,억제방사후신경간세포적조망.
Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C17.2 neural stem cells(NSCs) and explore the relationship between bFGF and Hes1,RBP-JK protein.Methods The cell viability was assessed using the MTT assay.After the cells attach to the flasks they were irradiated by the linear accelerator.And 5 min later,different concentrations of bFGF in accordance with the experimental design and cultured cells 48 h.Extracted total protein of each group and Western blot analysis showed the changes of the Hes1,RBP-JK protein.Results Compared with the control group,irradiation group cell growth was inhibited,the OD of 0 ng/ml bFGF group was 0.61±0.81,the OD of 80 ng/ml bFGF group was 1.21±1.01 and the control group was 1.51± 1.13.Compared with the control group,all groups showed statistically significant difference (P＜ 0.05).Western blot analysis showed that with the increasing concentrations of bFGF,RBP-JK expression gradually decreased,but Hes1 expression gradually increased.Conclusion Exogenous basic fibroblast growth factor (bFGF) can inhibit apoptosis of C17.2 NSCs.bFGF can regulate Notch signaling pathway downstream proteins Hes1,RBP-JK expression and inhibit neural stem cell apoptosis which were irradiated.
