CAJ | 학술논문

目的探讨EpCam与Wnt/β-catenin通路在结肠癌组织中表达分布情况及其临床病理意义,研究两者之间关系.方法回顾性分析用免疫组化方法检测的60例结肠癌组织、20例癌旁不典型增生组织、60正常结肠黏膜组织内EpCam与Wnt/β-catenin蛋白表达.结果 (1)正常结肠黏膜组织、癌旁组织、癌组织中EpCam阳性表达呈现明显上升趋势,分别为23.5％、62.3％、96.5％；随着正常结肠黏膜组织向癌组织的转化,β-catenin的胞膜表达阳性率依次降低,而胞质表达阳性率则依次增高,在低分化癌组织中甚至出现了核表达,EpCam强阳性表达及Wnt/β-catenin胞质阳性率与肿瘤组织学类型,肿瘤浸润深度,淋巴结转移均有关；(2)发现EpCam与Wnt/β-catenin呈正相关(r=0.653,P＜0.05),(3)高表达EpCam和Wnt/β-catenin患者结肠癌复发率明显增加,5年生存率明显降低.结论 EpCam与Wnt/β-catenin通路在结肠癌存在正相关性,EpCam和Wnt/β-catenin高表达与肿瘤浸润,转移及预后有密切关系.
목적 탐토EpCam여Wnt/β-catenin통로재결장암조직중표체분포정황급기림상병리의의,연구량자지간관계.방법 회고성분석용면역조화방법검측적60례결장암조직、20례암방불전형증생조직、60정상결장점막조직내EpCam여Wnt/β-catenin단백표체.결과 (1)정상결장점막조직、암방조직、암조직중EpCam양성표체정현명현상승추세,분별위23.5％、62.3％、96.5％；수착정상결장점막조직향암조직적전화,β-catenin적포막표체양성솔의차강저,이포질표체양성솔칙의차증고,재저분화암조직중심지출현료핵표체,EpCam강양성표체급Wnt/β-catenin포질양성솔여종류조직학류형,종류침윤심도,림파결전이균유관；(2)발현EpCam여Wnt/β-catenin정정상관(r=0.653,P＜0.05),(3)고표체EpCam화Wnt/β-catenin환자결장암복발솔명현증가,5년생존솔명현강저.결론 EpCam여Wnt/β-catenin통로재결장암존재정상관성,EpCam화Wnt/β-catenin고표체여종류침윤,전이급예후유밀절관계.
Objective EpCam and Wnt/ β-catenin pathway in colon carcinoma and its clinic-pathological significance of the distribution,studying the relationship between EpCam and Wnt / β-catenin.Methods Retrospective analysis detected by immunohistochemistry 60 cases of colon cancer,20 cases of adjacent atypical hyperplasia,60 normal colon tissue EpCam and Wnt / β-catenin protein expression.Results (1)normal colon tissue,cancer tissue and cancer tissue showed positive expression EpCam clear upward trend,were 23.5％,62.3％,96.5％ ; with normal colonic mucosa to cancer transformation,β-catenin in the membrane expression of the positive rate decreased,while the cytoplasm is followed by increased expression rate in poorly differentiated carcinoma or even nuclear expression,EpCam strong positive expression of Wnt / β-catenin cytoplasmic-positive rate of histological type,depth of invasion,lymph node metastasis; (2)the EpCam with the Wnt / β-catenin expression showed a positive correlation (r =0.653,P ＜0.05) ;(3)high expression EpCam and Wnt / β-catenin in patients with colon significant increase in cancer recurrence rate and 5-year survival rate was significantly reduced.Conclusion EpCam and Wnt / β-catenin pathway in colon cancer positively is correlated,EpCam and Wnt / β-catenin is connected with high expression and tumor invasion,metastasis and prognosis.