中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
1期
52-55
,共4页
血管内皮生长因子受体1/血液%血管内皮生长因子受体2/血液%血管内皮生长因子受体1/生物合成%血管内皮生长因子受体2/生物合成%先兆子(癎)/血液%先兆子(癎)/代谢
血管內皮生長因子受體1/血液%血管內皮生長因子受體2/血液%血管內皮生長因子受體1/生物閤成%血管內皮生長因子受體2/生物閤成%先兆子(癎)/血液%先兆子(癎)/代謝
혈관내피생장인자수체1/혈액%혈관내피생장인자수체2/혈액%혈관내피생장인자수체1/생물합성%혈관내피생장인자수체2/생물합성%선조자(간)/혈액%선조자(간)/대사
Vascular endothelial growth factor receptor-1/blood%Vascular endothelial growth factor receptor-2/blood%Vascular endothelial growth factor receptor-1/biosynthesis%Vascular endothelial growth factor receptor-2/biosynthesis%Pre-eclampsia/blood%Pre-ecl
目的 观察胎盘组织中血管内皮生长因子受体-1(VEGFR-1)、VEGFR-2、血浆中可溶性血管内皮生长因子受体-1(sVEGFR-1)及sVEGFR-2的表达水平,并探讨其在子(癎)前期的临床检测意义.方法 ELISA检测正常妊娠期妇女和子(癎)前期患者血浆中的sVEGFR-1和sVEGFR-2的表达；RT-PCR检测正常妊娠期妇女和子(癎)前期患者胎盘中VEGFR-1、VEGFR-2、sVEGFR-1和sVEGFR-2 mRNA的表达.结果 ELISA检测结果显示,正常妊娠组分娩后较分娩前血浆中sVEGFR-1[(4.55±0.31) ng/ml vs(12.33±1.52) ng/ml]水平降低,子(癎)前期组分娩后血浆中sVEGFR-1[(8.13±0.74) ng/ml vs (77.25±9.47) ng/ml]水平亦降低,两组分娩前后数据变化比较差异有统计学意义(P＜0.01)；正常妊娠组分娩前后血浆中sVEGFR-2分别为(8.74±1.24) ng/ml与(6.43±0.55) ng/ml,而子(癎)前期组分别为(5.69±0.75) ng/ml与(4.96±0.67) ng/ml；RT-PCR检测结果与其一致.结论 子(癎)前期患者分娩后VEGFR-1血浆水平迅速下降,而VEGFR-2持续低水平表达,提示VEG-FR-1可能与子(癎)前期发病率有关,而子(癎)前期患者血浆中VEGFR-2水平的降低可作为内皮功能障碍的标志.
目的 觀察胎盤組織中血管內皮生長因子受體-1(VEGFR-1)、VEGFR-2、血漿中可溶性血管內皮生長因子受體-1(sVEGFR-1)及sVEGFR-2的錶達水平,併探討其在子(癎)前期的臨床檢測意義.方法 ELISA檢測正常妊娠期婦女和子(癎)前期患者血漿中的sVEGFR-1和sVEGFR-2的錶達；RT-PCR檢測正常妊娠期婦女和子(癎)前期患者胎盤中VEGFR-1、VEGFR-2、sVEGFR-1和sVEGFR-2 mRNA的錶達.結果 ELISA檢測結果顯示,正常妊娠組分娩後較分娩前血漿中sVEGFR-1[(4.55±0.31) ng/ml vs(12.33±1.52) ng/ml]水平降低,子(癎)前期組分娩後血漿中sVEGFR-1[(8.13±0.74) ng/ml vs (77.25±9.47) ng/ml]水平亦降低,兩組分娩前後數據變化比較差異有統計學意義(P＜0.01)；正常妊娠組分娩前後血漿中sVEGFR-2分彆為(8.74±1.24) ng/ml與(6.43±0.55) ng/ml,而子(癎)前期組分彆為(5.69±0.75) ng/ml與(4.96±0.67) ng/ml；RT-PCR檢測結果與其一緻.結論 子(癎)前期患者分娩後VEGFR-1血漿水平迅速下降,而VEGFR-2持續低水平錶達,提示VEG-FR-1可能與子(癎)前期髮病率有關,而子(癎)前期患者血漿中VEGFR-2水平的降低可作為內皮功能障礙的標誌.
목적 관찰태반조직중혈관내피생장인자수체-1(VEGFR-1)、VEGFR-2、혈장중가용성혈관내피생장인자수체-1(sVEGFR-1)급sVEGFR-2적표체수평,병탐토기재자(간)전기적림상검측의의.방법 ELISA검측정상임신기부녀화자(간)전기환자혈장중적sVEGFR-1화sVEGFR-2적표체；RT-PCR검측정상임신기부녀화자(간)전기환자태반중VEGFR-1、VEGFR-2、sVEGFR-1화sVEGFR-2 mRNA적표체.결과 ELISA검측결과현시,정상임신조분면후교분면전혈장중sVEGFR-1[(4.55±0.31) ng/ml vs(12.33±1.52) ng/ml]수평강저,자(간)전기조분면후혈장중sVEGFR-1[(8.13±0.74) ng/ml vs (77.25±9.47) ng/ml]수평역강저,량조분면전후수거변화비교차이유통계학의의(P＜0.01)；정상임신조분면전후혈장중sVEGFR-2분별위(8.74±1.24) ng/ml여(6.43±0.55) ng/ml,이자(간)전기조분별위(5.69±0.75) ng/ml여(4.96±0.67) ng/ml；RT-PCR검측결과여기일치.결론 자(간)전기환자분면후VEGFR-1혈장수평신속하강,이VEGFR-2지속저수평표체,제시VEG-FR-1가능여자(간)전기발병솔유관,이자(간)전기환자혈장중VEGFR-2수평적강저가작위내피공능장애적표지.
Objective To analyze tissue vascular endothelial growth factor receptor 1 (VEGFR-1)and VEGFR-2 and their soluble form (sVEFGR-1 and sVEGFR-2) in the plasma in patients with preeclampsia,and to explore its clinical significance.Methods sVEGFR-1 and sVEGFR-2 expressions in plasma of normal pregnancy women and preeclampsia patients were detected by enzyme-linked immunosorbnent assay (ELISA) ; VEGFR-1,VEGFR-2,sVEGFR-1 and sVEGFR-2 mRNA expressions of normal pregnancy women and preeclampsia patients in placenta were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results ELISA results showed that the level of sVEGFR-1 in plasma of normal control group before and after delivery was (12.33 ± 1.52) ng/ml and (4.55 ± 0.31) ng/ml,respectively,while that of preeclampsia group was (77.25 ± 9.47) ng/ml and (8.13 ± 0.74) ng/ml,respectively; the differences between before and after delivery in the two groups were of statistical significance.The level of sVEGFR 2 in plasma of normal control group before and after delivery was (8.74 ± 1.24) ng/ml and (6.43± 0.55) ng/ml,respectively,while that of preeclampsia group was (5.69 ± 0.75) ng/ml and (4.96 ±0.67) ng/ml,respectively.RT-PCR results were consistent with ELISA results.Conclusions Rapid decrease of plasma sVEGFR-1 and continuously low-level expression of plasma sVEGFR-2 indicated that VEGFR-1 might be closely related to preeclampsia,and decrease of plasma sVEGFR-2 in preeclampsia women might be taken as a marker of endothelial cell function disorder.
