中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
2期
145-149
,共5页
骨折愈合%疾病模型,动物%桡骨骨折%骨形态发生蛋白质类/生物合成
骨摺愈閤%疾病模型,動物%橈骨骨摺%骨形態髮生蛋白質類/生物閤成
골절유합%질병모형,동물%뇨골골절%골형태발생단백질류/생물합성
Fracture healing%Disease models,animal%Radius fractures%Bone morphogenetic proteins/biosynthesis
目的 为临床骨缺损的实验研究建立一种客观的动物实验模型,检测骨缺损区骨形态发生蛋白-2(BMP-2)活性,并观察兔桡骨骨折的早期愈合情况.方法 选择新西兰大白兔,体重(2.8 ±0.2)kg,共72只,按随机数字表法分成A、B、C、D4个实验组,每组18只,分别建立兔左桡骨中段0.5、1.5、2.0、3.0 cm的骨缺损模型,造模同时将所截除骨段保留并取少量骨旁组织,造模时间记为0周,其后于1、3、4周时处死各组动物6只,取骨缺损周围组织,用western-blot法检测其BMP-2含量,并观察不同时间点的骨愈合情况.结果 建模1周时各实验组骨缺损区组织BMP-2成份和0周时相比分泌量明显增加(P＜0.05),3周时(A、B、C、D)4组分别增加了556.1％、385.9％、272.2％、171.2％.BMP-2成份分泌量均达峰值,4周时BMP-2含量较前明显减少(P＜0.05),同时,BMP-2的含量随骨缺损长度的增加而减少(P＜0.05).骨愈合情况和时间、骨缺损长度亦有关.结论 本研究成功建立了有可比性的兔桡骨骨缺损模型,这种较接近于临床实际的客观动物模型,为探索人类骨折愈合机制提供了动物实验的理论依据.BMP-2是骨折愈合重要的影响因子.
目的 為臨床骨缺損的實驗研究建立一種客觀的動物實驗模型,檢測骨缺損區骨形態髮生蛋白-2(BMP-2)活性,併觀察兔橈骨骨摺的早期愈閤情況.方法 選擇新西蘭大白兔,體重(2.8 ±0.2)kg,共72隻,按隨機數字錶法分成A、B、C、D4箇實驗組,每組18隻,分彆建立兔左橈骨中段0.5、1.5、2.0、3.0 cm的骨缺損模型,造模同時將所截除骨段保留併取少量骨徬組織,造模時間記為0週,其後于1、3、4週時處死各組動物6隻,取骨缺損週圍組織,用western-blot法檢測其BMP-2含量,併觀察不同時間點的骨愈閤情況.結果 建模1週時各實驗組骨缺損區組織BMP-2成份和0週時相比分泌量明顯增加(P＜0.05),3週時(A、B、C、D)4組分彆增加瞭556.1％、385.9％、272.2％、171.2％.BMP-2成份分泌量均達峰值,4週時BMP-2含量較前明顯減少(P＜0.05),同時,BMP-2的含量隨骨缺損長度的增加而減少(P＜0.05).骨愈閤情況和時間、骨缺損長度亦有關.結論 本研究成功建立瞭有可比性的兔橈骨骨缺損模型,這種較接近于臨床實際的客觀動物模型,為探索人類骨摺愈閤機製提供瞭動物實驗的理論依據.BMP-2是骨摺愈閤重要的影響因子.
목적 위림상골결손적실험연구건립일충객관적동물실험모형,검측골결손구골형태발생단백-2(BMP-2)활성,병관찰토뇨골골절적조기유합정황.방법 선택신서란대백토,체중(2.8 ±0.2)kg,공72지,안수궤수자표법분성A、B、C、D4개실험조,매조18지,분별건립토좌뇨골중단0.5、1.5、2.0、3.0 cm적골결손모형,조모동시장소절제골단보류병취소량골방조직,조모시간기위0주,기후우1、3、4주시처사각조동물6지,취골결손주위조직,용western-blot법검측기BMP-2함량,병관찰불동시간점적골유합정황.결과 건모1주시각실험조골결손구조직BMP-2성빈화0주시상비분비량명현증가(P＜0.05),3주시(A、B、C、D)4조분별증가료556.1％、385.9％、272.2％、171.2％.BMP-2성빈분비량균체봉치,4주시BMP-2함량교전명현감소(P＜0.05),동시,BMP-2적함량수골결손장도적증가이감소(P＜0.05).골유합정황화시간、골결손장도역유관.결론 본연구성공건립료유가비성적토뇨골골결손모형,저충교접근우림상실제적객관동물모형,위탐색인류골절유합궤제제공료동물실험적이론의거.BMP-2시골절유합중요적영향인자.
Objective To establish an objective animal experimental model for the experimental study of clinical bone defect,detect the BMP-2 activity in bone defect area,and observe the early healing situation of rabbit radial fracture.Methods A total of 72 New Zealand rabbits [weight/ each:(2.8 ±0.2)kg] were randomly divided into four experimental groups A,B,C,and D (18/each) with corresponding 0.5 cm,1.5 cm,2.0 cm,and 3.0 cm bone defect models abolished,respectively,in the middle of the rabbit left radius.By the time of modeling,all the bone segments were retained and a small amount of bone adjacent tissues were taken as E specimens of control group.Modeling time was recorded as 0 week,and six animals of each group were killed in 1 W,3 W,4 W later.Then the bone defect surrounding tissues were taken to check BMP-2 content by Western-blot detection,and the bone-healing situation was observed at different time.Results BMP-2 composition showed a significant increase in secretory volume of each group's bone defect area tissues after modeling one week,compared with E specimens of control group (P ＜ 0.05).BMP-2 composition secretion capacity even reached its peak in the third week four groups (A,B,C,D)increased by 556.1％,385.9％,272.2％,171.2％ respectively.and BMP-2 content reduced apparently in the fourth week than before(P ＜ 0.05).At the same time,BMP-2 content decreased with the addition of bone defect length(P ＜ 0.05).Also a correlation was shown between bone healing and time with the bone defect length.Conclusions A comparable rabbit radial bone defect model was successfully established.These objective animal models,which are closer to clinical reality,provide the theoretical basis of animal experiments to explore the mechanism of human fracture healing.
