中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
2期
170-173
,共4页
张谦%罗苏明%韩振魁%斯坎德尔·努尔买买提%刘江伟%刘妍芳%李鹏%郑建忠
張謙%囉囌明%韓振魁%斯坎德爾·努爾買買提%劉江偉%劉妍芳%李鵬%鄭建忠
장겸%라소명%한진괴%사감덕이·노이매매제%류강위%류연방%리붕%정건충
姜黄素/药理学%胃肿瘤/病理学%肿瘤细胞,培养的/药物作用%细胞增殖/药物作用%基质金属蛋白酶9/生物合成%基质金属蛋白酶9/遗传学%前列腺素内过氧化物合酶类/遗传学%前列腺素内过氧化物合酶类/生物合成
薑黃素/藥理學%胃腫瘤/病理學%腫瘤細胞,培養的/藥物作用%細胞增殖/藥物作用%基質金屬蛋白酶9/生物閤成%基質金屬蛋白酶9/遺傳學%前列腺素內過氧化物閤酶類/遺傳學%前列腺素內過氧化物閤酶類/生物閤成
강황소/약이학%위종류/병이학%종류세포,배양적/약물작용%세포증식/약물작용%기질금속단백매9/생물합성%기질금속단백매9/유전학%전렬선소내과양화물합매류/유전학%전렬선소내과양화물합매류/생물합성
Curcumin/pharmacology%Stomach neoplasms/pathology%Tumor cells,cultured/drug effects%Cell proliferation/drug effects%Matrix metalloproteinase 9/biosynthesis%Matrix metalloproteinase 9/ genetics%Prostaglandin-endoperoxide synthases/genetics%Prosta
目的 研究姜黄素在体外对人胃癌细胞BGC-823生长增殖的影响及其抗肿瘤的相关分子机制.方法 体外培养人胃癌BGC-823,MTT法检测姜黄素在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算IC50值；RT-PCR法检测其对COX-2、MMP-9 mRNA表达的影响.结果 MTF结果显示:相同干预浓度姜黄素抑制人胃癌细胞增殖,其24、48、72 h的IC50分别为:(62.192±7.802) μmol/L、(46.365±8.991) μmol/L和(45.503±9.126) μmol/L; RT-PCR结果显示:正常对照组及姜黄素干预组的人胃癌细胞BGC-823MMP-9 mRNA灰度值分别为:0.704±0.045,0.147 ±0.052(P＜0.05)；COX-2 mRNA灰度值分别为:0.721 ±0.060,0.190±0.027(P＜0.05).结论 姜黄素抗肿瘤机制可能通过抑制COX-2及MMP-9 mRNA的表达来抑制胃癌细胞增殖.
目的 研究薑黃素在體外對人胃癌細胞BGC-823生長增殖的影響及其抗腫瘤的相關分子機製.方法 體外培養人胃癌BGC-823,MTT法檢測薑黃素在相同濃度下,不同時間對于胃癌細胞增殖的影響,併計算IC50值；RT-PCR法檢測其對COX-2、MMP-9 mRNA錶達的影響.結果 MTF結果顯示:相同榦預濃度薑黃素抑製人胃癌細胞增殖,其24、48、72 h的IC50分彆為:(62.192±7.802) μmol/L、(46.365±8.991) μmol/L和(45.503±9.126) μmol/L; RT-PCR結果顯示:正常對照組及薑黃素榦預組的人胃癌細胞BGC-823MMP-9 mRNA灰度值分彆為:0.704±0.045,0.147 ±0.052(P＜0.05)；COX-2 mRNA灰度值分彆為:0.721 ±0.060,0.190±0.027(P＜0.05).結論 薑黃素抗腫瘤機製可能通過抑製COX-2及MMP-9 mRNA的錶達來抑製胃癌細胞增殖.
목적 연구강황소재체외대인위암세포BGC-823생장증식적영향급기항종류적상관분자궤제.방법 체외배양인위암BGC-823,MTT법검측강황소재상동농도하,불동시간대우위암세포증식적영향,병계산IC50치；RT-PCR법검측기대COX-2、MMP-9 mRNA표체적영향.결과 MTF결과현시:상동간예농도강황소억제인위암세포증식,기24、48、72 h적IC50분별위:(62.192±7.802) μmol/L、(46.365±8.991) μmol/L화(45.503±9.126) μmol/L; RT-PCR결과현시:정상대조조급강황소간예조적인위암세포BGC-823MMP-9 mRNA회도치분별위:0.704±0.045,0.147 ±0.052(P＜0.05)；COX-2 mRNA회도치분별위:0.721 ±0.060,0.190±0.027(P＜0.05).결론 강황소항종류궤제가능통과억제COX-2급MMP-9 mRNA적표체래억제위암세포증식.
Objective To investigate modulating effects of curcumin on proliferation and the molecular mechanism in gastric carcinoma cell line BGC-823.Methods Cells were incubated with curcumin in the same concentration for 24 h,48 h,and 72 h.The cell viability was measured by MTT assay.IC50 was calculated.RT-PCR was used to evaluate the expression of COX-2 and MMP-9.Results The BGC-823 cell viability was apparently inhibited by curcumin with a inhibitory effect in the time-dependent and dosedependent manner.The IC50 of the BGC-823 cell line was (62.192 ±7.802)μmol/L (24 h),(46.365 ±8.991) μmol/L (48 h),and (45.503 ±9.126) μmol/L (72 h).RT-PCR showed the expressions of COX-2 and MMP-9 were down-regulated.The expression of MMP-9 mRNA in the BGC-823 cells was 0.704 ±0.045 for the normal control group and 0.147 ± 0.052 for the curcumin-treated group wit a statistical significance (P ＜ 0.05) ; COX-2 were 0.721 ± 0.060 for the normal control group and 0.190 ± 0.027 for the curcumin-treated group with a statistical significance (P ＜ 0.05).Conclusions The molecular mechanism that curcumin inhibited the proliferation of tumor cells might be that it inhibited preferentially the expressions of COX-2 and MMP-9 in gastric carcinoma cell line.
