CAJ | 학술논문

目的探讨信号转导和转录活化因子3(Signal transduction and transcription activation factor 3,STAT3)和CD34在胃癌发生发展中的表达及意义.方法选取2006年1月至2011年12月本院病理科标本120例.按诊断分慢性胃炎组、肠型化生组、不典型增生组、胃癌组,每组30例.并用免疫组化法检测各组STAT3和CD34的表达.结果慢性胃炎组、肠型化生组、不典型增生组、胃癌组STAT3阳性表达率分别为16.67％、93.33％、83.33％、86.67％；慢性胃炎组与肠型化生组、不典型增生组、胃癌组之间差异均有统计学意义(x2=157.55,133.33,59.80,P＜0.01)；肠型化生组、不典型增生组、胃癌组之间差异无统计学意义(P＞0.05).慢性胃炎组CD34表达的微血管密度(MVD)为13.01±9.19,肠化生组为13.09±9.37,不典型增生组为21.38 ±8.51,胃癌组为28.64±8.62；慢性胃炎组、肠型化生组与不典型增生组、胃癌组之间比较差异均有统计学意义(P＜0.01)；慢性胃炎组与肠型化生组之间差异无统计学意义(P＞0.05),不典型增生组与胃癌组差异无统计学意义(P＞0.05).结论 STAT3表达明显升高与胃癌的发生、发展有关,CD34增加与胃癌的发生有关；联合检测STAT3和CD34的表达情况有助胃癌的早期诊断.
목적 탐토신호전도화전록활화인자3(Signal transduction and transcription activation factor 3,STAT3)화CD34재위암발생발전중적표체급의의.방법 선취2006년1월지2011년12월본원병이과표본120례.안진단분만성위염조、장형화생조、불전형증생조、위암조,매조30례.병용면역조화법검측각조STAT3화CD34적표체.결과 만성위염조、장형화생조、불전형증생조、위암조STAT3양성표체솔분별위16.67％、93.33％、83.33％、86.67％；만성위염조여장형화생조、불전형증생조、위암조지간차이균유통계학의의(x2=157.55,133.33,59.80,P＜0.01)；장형화생조、불전형증생조、위암조지간차이무통계학의의(P＞0.05).만성위염조CD34표체적미혈관밀도(MVD)위13.01±9.19,장화생조위13.09±9.37,불전형증생조위21.38 ±8.51,위암조위28.64±8.62；만성위염조、장형화생조여불전형증생조、위암조지간비교차이균유통계학의의(P＜0.01)；만성위염조여장형화생조지간차이무통계학의의(P＞0.05),불전형증생조여위암조차이무통계학의의(P＞0.05).결론 STAT3표체명현승고여위암적발생、발전유관,CD34증가여위암적발생유관；연합검측STAT3화CD34적표체정황유조위암적조기진단.
Objective To investigate the expression and significance of signal transduction and transcription activation factor 3 (STAT3) and CD34 in gastric carcinogenesis.Methods Pathological tissue specimens was collected in the Department of Pathology,The Second People's Hospital,Shenzhen from January 2006 to December 2011.According to the pathological diagnosis,four groups were set up including chronic gastritis,intestinal metaplasia,displasia,and gastric cancer groups (n =30 cases/goup).STAT3 and CD34 were detected by immunohistochemistry.Results The positive expression rates of STAT3 were 16.67％ in the chronic gastritis group,93.33％ in the intestinal metaplasia group,83.33％ in the displasia group,and 86.67％ in the gastric cancer group with a statistically significant difference among four groups (x2 =157.55,133.33,59.80,P ＜ 0.01),and,however,without statistically significant difference among the intestinal metaplasia,displasia,and gastric cancer groups (P ＞ 0.05).The expressions of CD34 were 13.01 ±9.19 in the chronic gastritis group,13.09 ±9.37 in the intestinal metaplasia group,21.38 ± 8.51 in the displasia group,and 28.64 ± 8.62 in the gastric cancer group.The microvascular density showed a statistically significant difference among four groups (P ＜0.01) ; however,no difference was found between the chronic gastritis and intestinal metaplasia groups (P ＞ 0.05) and between the displasia and gastric cancer groups (P ＞ 0.05).Conclusions The expressions of STAT3 were increased significantly,and were related to the occurrence and development of gastric cancer.The increased microvascular density was related to the occurrence of gastric cancer.The combination of expression changes of STAT3 and CD34 will benefit the early diagnosis of gastric cancer.