中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
5期
599-603
,共5页
金玲%刘卫武%幸梓%段于峰%李洪亮
金玲%劉衛武%倖梓%段于峰%李洪亮
금령%류위무%행재%단우봉%리홍량
高血压%蛋白质组学%质谱分析法%少数民族
高血壓%蛋白質組學%質譜分析法%少數民族
고혈압%단백질조학%질보분석법%소수민족
Hypertension%Proteomics%Mass spectrometry%Minority groups
目的 分析怀化侗族高血压患者血淋巴细胞蛋白质谱,寻找高血压患者血淋巴细胞差异蛋白质,研究其在怀化侗族高血压发病机制中的意义.方法 应用MALDI-TOF-MS技术检测130例血淋巴细胞标本(侗族高血压50例,汉族高血压40例,侗族正常人群40例)的蛋白质质谱,用MatrixScience公司的Mascot对蛋白质质谱数据进行数据库查询比对,并搜索鉴定蛋白.结果 三组均获得重复性好的血淋巴细胞蛋白质双向凝胶电泳图谱.其中3个差异表达蛋白点经质谱鉴定.与侗族正常人比较,侗族高血压蛋白质二硫化物异构酶5、热休克蛋白27两个蛋白表达上调(P＜0.01),Chain A,Structure Of Haemoglobin In The Deoxy Quaternary State With Ligand Bound At The Alpha Haems表达差异无统计学意义(P＞0.05)；汉族高血压上述三个蛋白点表达均上调(P＜0.01).结论 蛋白质二硫化物异构酶5、热休克蛋白27在侗族、汉族高血压中高表达,推测这两个蛋白可能与高血压的发生、发展有关.
目的 分析懷化侗族高血壓患者血淋巴細胞蛋白質譜,尋找高血壓患者血淋巴細胞差異蛋白質,研究其在懷化侗族高血壓髮病機製中的意義.方法 應用MALDI-TOF-MS技術檢測130例血淋巴細胞標本(侗族高血壓50例,漢族高血壓40例,侗族正常人群40例)的蛋白質質譜,用MatrixScience公司的Mascot對蛋白質質譜數據進行數據庫查詢比對,併搜索鑒定蛋白.結果 三組均穫得重複性好的血淋巴細胞蛋白質雙嚮凝膠電泳圖譜.其中3箇差異錶達蛋白點經質譜鑒定.與侗族正常人比較,侗族高血壓蛋白質二硫化物異構酶5、熱休剋蛋白27兩箇蛋白錶達上調(P＜0.01),Chain A,Structure Of Haemoglobin In The Deoxy Quaternary State With Ligand Bound At The Alpha Haems錶達差異無統計學意義(P＞0.05)；漢族高血壓上述三箇蛋白點錶達均上調(P＜0.01).結論 蛋白質二硫化物異構酶5、熱休剋蛋白27在侗族、漢族高血壓中高錶達,推測這兩箇蛋白可能與高血壓的髮生、髮展有關.
목적 분석부화동족고혈압환자혈림파세포단백질보,심조고혈압환자혈림파세포차이단백질,연구기재부화동족고혈압발병궤제중적의의.방법 응용MALDI-TOF-MS기술검측130례혈림파세포표본(동족고혈압50례,한족고혈압40례,동족정상인군40례)적단백질질보,용MatrixScience공사적Mascot대단백질질보수거진행수거고사순비대,병수색감정단백.결과 삼조균획득중복성호적혈림파세포단백질쌍향응효전영도보.기중3개차이표체단백점경질보감정.여동족정상인비교,동족고혈압단백질이류화물이구매5、열휴극단백27량개단백표체상조(P＜0.01),Chain A,Structure Of Haemoglobin In The Deoxy Quaternary State With Ligand Bound At The Alpha Haems표체차이무통계학의의(P＞0.05)；한족고혈압상술삼개단백점표체균상조(P＜0.01).결론 단백질이류화물이구매5、열휴극단백27재동족、한족고혈압중고표체,추측저량개단백가능여고혈압적발생、발전유관.
Objective To explore lymphocyte differentially expressed protein profile and its pathogenic significance in Huaihua Dong peoples with hypertension.Methods The lymphocyte protein profiles among 130 cases of blood specimen were detected and identified with matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS) and Mascot search against protein database.Results The reproducible protein-maps of two-dimensional gel electrophoresis were obtained among three groups.Compared with Dong normal peoples,the protein disulfide isomerase-related protein 5 and heat shock protein 27 showed higher expressions in the Dong peoples with hypertension (P ＜ 0.01),but chain A,structure of haemoglobin in the deoxy quaternary state with ligand bound at the alpha haems is no statistical significance(P ＞0.05).Those three proteins were upregulated in Han peoples with hypertension (P ＜0.01).Conclusions The up-regulation of the protein disulfide isomerase-related protein 5 and heat shock protein 27 in the Dong and Han peoples with hypertension suggest that those two proteins might be associated with the occurrence and development of hypertension.
