中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
7期
885-889
,共5页
曹伟军%徐亦君%徐兆军%袁捷%张振玉
曹偉軍%徐亦君%徐兆軍%袁捷%張振玉
조위군%서역군%서조군%원첩%장진옥
结直肠肿瘤/病理学%细胞增殖%RNA干扰
結直腸腫瘤/病理學%細胞增殖%RNA榦擾
결직장종류/병이학%세포증식%RNA간우
Colorectal neoplasms/pathology%Cell proliferation%RNA interference
目的 观察Yes相关蛋白(YAP)在结直肠癌中的表达,并分析其对结直肠癌细胞增殖的影响.方法 收集结直肠癌组织及癌旁正常组织各94例,采用免疫组化方法检测YAP蛋白的表达.利用siRNA降低结直肠癌细胞HCT116中YAP的表达,运用MTr比色法检测结直肠癌细胞增殖的变化.结果 YAP蛋白在结直肠癌组织中的阳性率为69.1％(65/94),明显高于癌旁正常组织的22.3％ (21/94),差异有统计学意义(P＜0.001).YAP蛋白的表达与结直肠癌的TNM分期及淋巴结转移有关(P＜0.05),而与患者性别、年龄、肿瘤部位及分化程度无关(P＞0.05).利用脂质体将靶向YAP的siRNA转染HCT116后,实验组YAP mRNA和蛋白表达量分别下降(78.2±2.1)％和(81.7±1.5)％,差异有统计学意义(t=67.55,91.601,P＜0.01)；HCT116的增殖受到显著抑制,48、72、96 h实验组的细胞生长抑制率分别为(28.1±1.6)％、(34.7±2.4)％、(24.7±1.2)％.结论 YAP在结直肠癌中高表达,降低其表达能显著抑制结直肠癌细胞的增殖.YAP可作为结直肠癌分子诊断和基因治疗的一个新的候选靶点.
目的 觀察Yes相關蛋白(YAP)在結直腸癌中的錶達,併分析其對結直腸癌細胞增殖的影響.方法 收集結直腸癌組織及癌徬正常組織各94例,採用免疫組化方法檢測YAP蛋白的錶達.利用siRNA降低結直腸癌細胞HCT116中YAP的錶達,運用MTr比色法檢測結直腸癌細胞增殖的變化.結果 YAP蛋白在結直腸癌組織中的暘性率為69.1％(65/94),明顯高于癌徬正常組織的22.3％ (21/94),差異有統計學意義(P＜0.001).YAP蛋白的錶達與結直腸癌的TNM分期及淋巴結轉移有關(P＜0.05),而與患者性彆、年齡、腫瘤部位及分化程度無關(P＞0.05).利用脂質體將靶嚮YAP的siRNA轉染HCT116後,實驗組YAP mRNA和蛋白錶達量分彆下降(78.2±2.1)％和(81.7±1.5)％,差異有統計學意義(t=67.55,91.601,P＜0.01)；HCT116的增殖受到顯著抑製,48、72、96 h實驗組的細胞生長抑製率分彆為(28.1±1.6)％、(34.7±2.4)％、(24.7±1.2)％.結論 YAP在結直腸癌中高錶達,降低其錶達能顯著抑製結直腸癌細胞的增殖.YAP可作為結直腸癌分子診斷和基因治療的一箇新的候選靶點.
목적 관찰Yes상관단백(YAP)재결직장암중적표체,병분석기대결직장암세포증식적영향.방법 수집결직장암조직급암방정상조직각94례,채용면역조화방법검측YAP단백적표체.이용siRNA강저결직장암세포HCT116중YAP적표체,운용MTr비색법검측결직장암세포증식적변화.결과 YAP단백재결직장암조직중적양성솔위69.1％(65/94),명현고우암방정상조직적22.3％ (21/94),차이유통계학의의(P＜0.001).YAP단백적표체여결직장암적TNM분기급림파결전이유관(P＜0.05),이여환자성별、년령、종류부위급분화정도무관(P＞0.05).이용지질체장파향YAP적siRNA전염HCT116후,실험조YAP mRNA화단백표체량분별하강(78.2±2.1)％화(81.7±1.5)％,차이유통계학의의(t=67.55,91.601,P＜0.01)；HCT116적증식수도현저억제,48、72、96 h실험조적세포생장억제솔분별위(28.1±1.6)％、(34.7±2.4)％、(24.7±1.2)％.결론 YAP재결직장암중고표체,강저기표체능현저억제결직장암세포적증식.YAP가작위결직장암분자진단화기인치료적일개신적후선파점.
Objective To evaluate the expression of Yes-associated protein (YAP) in colorectal carcinoma and analyze its influence on tumor cell proliferation.Methods The expressions of YAP in 94paired colorectal carcinomas and pericancerous normal tissues were detected by using immunohistochemistry method.The expressions of YAP in colorectal carcinoma cell line HCT116 were inhibited with a YAP-spe-cific siRNA.Cell proliferation was then determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay.Results The positive rate of YAP in colorectal carcinomas was significantly higher than that in pericancerous normal tissues [69.1％ (65/94) vs 22.3 ％ (21/94),P ＜ 0.001].The expression of YAP was associated with tumor Node Metastasis(TNM) stage and lymph node metastasis(P ＜0.05),but not associated with gender,age,tumor location and histological grade(P ＞0.05).After YAP-specific siR-NA was transfected into HCT116 using lipofectamine,the expression of YAP mRNA and protein in the experimental group were reduced by (78.2 ±2.1)％ and (81.7 ± 1.5)％,respectively,with a statistically significant difference (t =67.55,91.601,P ＜0.01).The growth of HCT116 was significantly inhibited and the reduced rate of cell proliferation was (28.1 ± 1.6) ％,(34.7 ± 2.4) ％ and (24.7 ± 1.2) ％ at the time point of 48 h,72 h and 96 h,respectively.Conclusions Expression of YAP was upregulated in colorectal carcinomas and downregulation of YAP expression could inhibit growth of colorectal carcinoma cells.YAP can be used as a new candidate target for diagnosis and treatment of a colorectal carcinoma.
