CAJ | 학술논문

目的检测Wnt-5a基因和基因产物在原发性肝细胞癌中的表达,探讨Wnt-5a基因在肝癌发生过程中的作用和临床意义.方法采用实时定量PCR技术,检测Wnt-5a mRNA在26例新鲜肝细胞癌和癌旁组织中的含量;用免疫组化法观察Wnt-5a蛋白在85例肝细胞癌和癌旁组织、15例肝硬化组织中的表达.结果 Wnt-5a mRNA在肝癌及癌旁组织中的相对含量分别为0.102 127±0.158 620和0.020 106±0.022 075,两者间差异有统计学意义(P＜0.05);Wnt-5a蛋白在肝细胞癌、癌旁组织和肝硬化中的阳性率分别为21.2％ (18/85)、81.26％ (69/85)和86.7％ (13/15),Wnt-5a在肝癌组织中的阳性表达显著低于肝硬化和癌旁组织,差异有统计学意义(P ＜0.01);Wnt-5a蛋白在肝癌中的表达与肿瘤的TNM分期和血清甲胎蛋白(AFP)含量有关(P＜0.05).结论 Wnt-5a基因在HCC基因转录中呈上调表达,而在蛋白水平显示为缺失和下调表达,提示肿瘤细胞是在蛋白翻译水平而不是转录水平干扰了Wnt-5a基因功能.
목적 검측Wnt-5a기인화기인산물재원발성간세포암중적표체,탐토Wnt-5a기인재간암발생과정중적작용화림상의의.방법 채용실시정량PCR기술,검측Wnt-5a mRNA재26례신선간세포암화암방조직중적함량;용면역조화법관찰Wnt-5a단백재85례간세포암화암방조직、15례간경화조직중적표체.결과 Wnt-5a mRNA재간암급암방조직중적상대함량분별위0.102 127±0.158 620화0.020 106±0.022 075,량자간차이유통계학의의(P＜0.05);Wnt-5a단백재간세포암、암방조직화간경화중적양성솔분별위21.2％ (18/85)、81.26％ (69/85)화86.7％ (13/15),Wnt-5a재간암조직중적양성표체현저저우간경화화암방조직,차이유통계학의의(P ＜0.01);Wnt-5a단백재간암중적표체여종류적TNM분기화혈청갑태단백(AFP)함량유관(P＜0.05).결론 Wnt-5a기인재HCC기인전록중정상조표체,이재단백수평현시위결실화하조표체,제시종류세포시재단백번역수평이불시전록수평간우료Wnt-5a기인공능.
Objective To investigate the expression of Wnt-5a gene in primary hepatocellular carcinoma (HCC) and to expose its role and clinical significance in the development of HCC.Methods Real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed in 26 fresh HCC samples and the corresponding para-carcinoma tissues to detect mRNA expression of Wnt-5a gene.Wnt-5a protein was detected with immunohistochemical method in paraffin embedding tissues of 85 cases of HCCs and the corresponding para-carcinoma tissues,and 15 cases of hepatic cirrhosis.Results RT-PCR analysis showed that Wnt-5a mRNA (0.102 127 ±0.158 620) in the HCC tissues was more than that (0.020 106 ±0.022 075) in the para-carcinoma tissues (P＜0.05).The positive expression rate of Wnt-5a protein in HCC,para-carcinoma,and hepatic cirrhosis tissues were 21.2％ (18/85),81.26％ (69/85),and 86.7％ (13/15),respectively.The positive rate of Wnt-5a was significantly lower in the HCC than in the para-carcinoma and hepatic cirrhosis tissues (P ＜ 0.01).The expression of Wnt-5a was significantly associated with lower tumor node metastasis (TNM) stages and small alpha fetoproteins (AFP) content of blood serum (P ＜0.05).Conclusions The high expression of Wnt-5a mRNA was found in the gene transcription of HCC,while Wnt-5a protein was absent or low in HCC.It was suggested that the roles of Wnt-5a was interfered at the protein level rather than the transcriptional level in the HCC.