中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
9期
1221-1223
,共3页
多态现象,遗传%基因型%糖尿病,妊娠/遗传学%脂联素/遗传学%多态性,限制性片段长度%聚合酶链反应%广西[壮族自治区]
多態現象,遺傳%基因型%糖尿病,妊娠/遺傳學%脂聯素/遺傳學%多態性,限製性片段長度%聚閤酶鏈反應%廣西[壯族自治區]
다태현상,유전%기인형%당뇨병,임신/유전학%지련소/유전학%다태성,한제성편단장도%취합매련반응%엄서[장족자치구]
Polymorphism,genetic%Genotype%Diabetes,gestational/genetics%Adiponectin/genetics%Polymorphism,restriction fragment length%Polymerase chain reaction%GUANGXI
目的 研究脂联素基因单核苷酸多态性(SNP) +45T/G和+276G/T两个位点与广西地区壮族妇女妊娠期糖尿病的关系.方法 采用聚合酶链反应-限制性片段长度多态性技术对98例妊娠期糖尿病(GDM)孕妇(GDM组)及135例糖耐量正常孕妇(对照组)脂联素基因启动予多态性位点(+ 45T/G和+276G/T)进行基因分析.结果 GDM组SNP+ 45 T/G多态性的TG、GG基因型频率显著高于对照组(P＜0.05),G等位基因频率显著高于对照组(P＜0.05),GDM组SNP+ 276 G/T多态性的基因型频率和等位基因频率与正常对照组比较差异无统计学意义(P＞0.05).GDM孕妇SNP+ 45T/G多态性位点TG+ GG基因型组甘油三酯水平高于TT基因型组(P＜0.05),血清脂联素水平低于TT基因型组(P＜0.05).结论 脂联素基因+45T/G位点多态性可能与GDM的发生有关,G等位基因在GDM孕妇中分布频率增加,T→G突变可能通过降低血清脂联素水平参与GDM的发生.检测孕妇该位点,可预测GDM的发生风险.
目的 研究脂聯素基因單覈苷痠多態性(SNP) +45T/G和+276G/T兩箇位點與廣西地區壯族婦女妊娠期糖尿病的關繫.方法 採用聚閤酶鏈反應-限製性片段長度多態性技術對98例妊娠期糖尿病(GDM)孕婦(GDM組)及135例糖耐量正常孕婦(對照組)脂聯素基因啟動予多態性位點(+ 45T/G和+276G/T)進行基因分析.結果 GDM組SNP+ 45 T/G多態性的TG、GG基因型頻率顯著高于對照組(P＜0.05),G等位基因頻率顯著高于對照組(P＜0.05),GDM組SNP+ 276 G/T多態性的基因型頻率和等位基因頻率與正常對照組比較差異無統計學意義(P＞0.05).GDM孕婦SNP+ 45T/G多態性位點TG+ GG基因型組甘油三酯水平高于TT基因型組(P＜0.05),血清脂聯素水平低于TT基因型組(P＜0.05).結論 脂聯素基因+45T/G位點多態性可能與GDM的髮生有關,G等位基因在GDM孕婦中分佈頻率增加,T→G突變可能通過降低血清脂聯素水平參與GDM的髮生.檢測孕婦該位點,可預測GDM的髮生風險.
목적 연구지련소기인단핵감산다태성(SNP) +45T/G화+276G/T량개위점여엄서지구장족부녀임신기당뇨병적관계.방법 채용취합매련반응-한제성편단장도다태성기술대98례임신기당뇨병(GDM)잉부(GDM조)급135례당내량정상잉부(대조조)지련소기인계동여다태성위점(+ 45T/G화+276G/T)진행기인분석.결과 GDM조SNP+ 45 T/G다태성적TG、GG기인형빈솔현저고우대조조(P＜0.05),G등위기인빈솔현저고우대조조(P＜0.05),GDM조SNP+ 276 G/T다태성적기인형빈솔화등위기인빈솔여정상대조조비교차이무통계학의의(P＞0.05).GDM잉부SNP+ 45T/G다태성위점TG+ GG기인형조감유삼지수평고우TT기인형조(P＜0.05),혈청지련소수평저우TT기인형조(P＜0.05).결론 지련소기인+45T/G위점다태성가능여GDM적발생유관,G등위기인재GDM잉부중분포빈솔증가,T→G돌변가능통과강저혈청지련소수평삼여GDM적발생.검측잉부해위점,가예측GDM적발생풍험.
Objective To investigate the association of single nuchotide polymorphism (SNP) + 45 T/G and + 276 G/T in the adiponectin gene with type 2 diabetes in Guangxi Zhuang pregnant women.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the distributions of allele and genotype frequencies of SNP + 45T/G polymorphism and SNP + 276 G/T polymorphism in adiponect in gene in 98 type 2 diabetic patients and 135 normal control subjects.Results The TG,GG genotype frequencies of the SNP +45T/G were higher in gestational diabetes mellitus (GDM) than those in controls (P ＜ 0.05).The G-allele frequency was significantly higher than those in controls (P ＜ 0.05).The distribution of genotype and allele frequencies of the SNP + 276 G/T polymorphism was not different in diabetes and control groups (P ＞ 0.05).Women carrying the TG and GG allele of SNP +45T/G showed higher TG (P ＜0.05),and lower APN levels than those with TT genotype (P ＜0.05).Conclusions G allele of SNP +45T/G was related to GDM possibly.T→G variation might involve in the occurrence of GDM by reducing serum diponectin levels.Monitoring this site in pregnant women could predict the risk of GDM.
