中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
10期
1303-1308
,共6页
李晓媚%何玉莲%黄程%方伟进%熊燕
李曉媚%何玉蓮%黃程%方偉進%熊燕
리효미%하옥련%황정%방위진%웅연
饥饿%肌,骨骼%大鼠,Sprague-Dawley%心血管系统%血管舒张
饑餓%肌,骨骼%大鼠,Sprague-Dawley%心血管繫統%血管舒張
기아%기,골격%대서,Sprague-Dawley%심혈관계통%혈관서장
Hunger%Muscle,skeletal%Rats,Sprague-Dawley%Cardiovascular system%Vasodilation
目的 观察不同时程的饥饿对大鼠骨骼肌收缩与心、血管功能的影响.方法 取成年雄性SD大鼠,制备不同时程的饥饿动物模型(实验分正常对照组、饥饿12h组、饥饿24 h组、饥饿48 h组);大鼠经过上述处理后,麻醉下迅速分离心脏、胸主动脉、比目鱼肌和趾长伸肌,并分别连接压力或张力换能器以及相应的实验装置和数据记录系统.心脏用Langendorff灌流,先记录左心室收缩压、舒张末期压和左室压力的变化速率(±dP/dt max)等心功能指标,再进行缺血-再灌注实验,并观测上述心功能指标的变化;用器官浴槽法检测血管环收缩与内皮依赖性舒张反应;用电刺激法分别检测比目鱼肌和趾长伸肌单次和强直收缩的最大张力.结果 与正常对照组比较,饥饿24h和48 h均明显降低心脏收缩期左室内压上升的最大变化速率(+dP/dt max,P＜0.01);在遭受心肌缺血再灌注后,饥饿48 h明显延长心脏复跳时间[(24.3 ±7.0)min vs(14.0±2.9) min,P＜0.05],并升高左心室平均舒张压和舒张期左室内压下降的最大变化速度(-dP/dt max)(P＜0.05),但到再灌60 min恢复正常;饥饿48 h也增加离体血管环对低浓度乙酰胆碱(3×10-8 ACh)诱导的内皮依赖性舒张反应(P＜0.05),但不增加ACh诱导的最大舒张反应,饥饿12～48 h均不影响血管环的收缩反应;饥饿48 h还增强比目鱼肌对电刺激诱导的强直收缩张力[(7.01±1.22) N/cm2 vs (5.50 ±0.75) N/cm2,P ＜0.05].结论 饥饿24 ～48 h对大鼠心脏功能有一定的抑制作用,但饥饿48 h增加ACh诱导血管内皮依赖性舒张反应的敏感性,并增强比目鱼肌对电刺激反应的收缩功能.
目的 觀察不同時程的饑餓對大鼠骨骼肌收縮與心、血管功能的影響.方法 取成年雄性SD大鼠,製備不同時程的饑餓動物模型(實驗分正常對照組、饑餓12h組、饑餓24 h組、饑餓48 h組);大鼠經過上述處理後,痳醉下迅速分離心髒、胸主動脈、比目魚肌和趾長伸肌,併分彆連接壓力或張力換能器以及相應的實驗裝置和數據記錄繫統.心髒用Langendorff灌流,先記錄左心室收縮壓、舒張末期壓和左室壓力的變化速率(±dP/dt max)等心功能指標,再進行缺血-再灌註實驗,併觀測上述心功能指標的變化;用器官浴槽法檢測血管環收縮與內皮依賴性舒張反應;用電刺激法分彆檢測比目魚肌和趾長伸肌單次和彊直收縮的最大張力.結果 與正常對照組比較,饑餓24h和48 h均明顯降低心髒收縮期左室內壓上升的最大變化速率(+dP/dt max,P＜0.01);在遭受心肌缺血再灌註後,饑餓48 h明顯延長心髒複跳時間[(24.3 ±7.0)min vs(14.0±2.9) min,P＜0.05],併升高左心室平均舒張壓和舒張期左室內壓下降的最大變化速度(-dP/dt max)(P＜0.05),但到再灌60 min恢複正常;饑餓48 h也增加離體血管環對低濃度乙酰膽堿(3×10-8 ACh)誘導的內皮依賴性舒張反應(P＜0.05),但不增加ACh誘導的最大舒張反應,饑餓12～48 h均不影響血管環的收縮反應;饑餓48 h還增彊比目魚肌對電刺激誘導的彊直收縮張力[(7.01±1.22) N/cm2 vs (5.50 ±0.75) N/cm2,P ＜0.05].結論 饑餓24 ～48 h對大鼠心髒功能有一定的抑製作用,但饑餓48 h增加ACh誘導血管內皮依賴性舒張反應的敏感性,併增彊比目魚肌對電刺激反應的收縮功能.
목적 관찰불동시정적기아대대서골격기수축여심、혈관공능적영향.방법 취성년웅성SD대서,제비불동시정적기아동물모형(실험분정상대조조、기아12h조、기아24 h조、기아48 h조);대서경과상술처리후,마취하신속분리심장、흉주동맥、비목어기화지장신기,병분별련접압력혹장력환능기이급상응적실험장치화수거기록계통.심장용Langendorff관류,선기록좌심실수축압、서장말기압화좌실압력적변화속솔(±dP/dt max)등심공능지표,재진행결혈-재관주실험,병관측상술심공능지표적변화;용기관욕조법검측혈관배수축여내피의뢰성서장반응;용전자격법분별검측비목어기화지장신기단차화강직수축적최대장력.결과 여정상대조조비교,기아24h화48 h균명현강저심장수축기좌실내압상승적최대변화속솔(+dP/dt max,P＜0.01);재조수심기결혈재관주후,기아48 h명현연장심장복도시간[(24.3 ±7.0)min vs(14.0±2.9) min,P＜0.05],병승고좌심실평균서장압화서장기좌실내압하강적최대변화속도(-dP/dt max)(P＜0.05),단도재관60 min회복정상;기아48 h야증가리체혈관배대저농도을선담감(3×10-8 ACh)유도적내피의뢰성서장반응(P＜0.05),단불증가ACh유도적최대서장반응,기아12～48 h균불영향혈관배적수축반응;기아48 h환증강비목어기대전자격유도적강직수축장력[(7.01±1.22) N/cm2 vs (5.50 ±0.75) N/cm2,P ＜0.05].결론 기아24 ～48 h대대서심장공능유일정적억제작용,단기아48 h증가ACh유도혈관내피의뢰성서장반응적민감성,병증강비목어기대전자격반응적수축공능.
Objective To investigate the effects of fasting with different time courses on the contraction of skeletal muscle and cardiovascular function in Sprague-Dawley (SD) rats.Methods The healthy and male SD rats were randomly divided into (1) control,(2) 12 h fasting,(3) 24 h fasting,and (4) 48 h fasting groups.After animal models of fasting with different time courses were established,the heart,thoracic aorta,soleus,and extensor digitorum longus (EDL) were rapidly isolated from rats under anesthesia and then fixed into the respective organ perfusion system that was connected to physiological recorders and data acquisition system.Langendorff heart perfusion was used to detect some parameters of cardiac function,including left ventricular systolic pressure (LVSP),left ventricular end-diastolic pressure (LVEDP),and maximum ascending and declining rate of left ventricular pressure (± dP/dt max).Then,the heart was suffered from 30 min ischemia followed by 60 min reperfusion,the changes in above cardiac function parameters were recorded again.The contraction response to potassium chloride or phenylephrine and endothelium-dependent relation responses to acetylcholine (ACh) of aortic rings were measured with organ bath.The changes of twitch tension and titanic tension of soleus and EDL were examined to reflect contractive function of skeletal muscle.Results The + dP/dt max was significantly reduced in 24 h and 48 h fasting groups compared to the control (P ＜ 0.01).After ischemia-reperfusion,the heart re-beat time was significantly longer [(24.3 ± 7.0)min vs (14.0 ± 2.9)min,P ＜0.05],both mLVDP and mLVP were significantly higher in 48 h and 24 h fasting groups than control group (P ＜ 0.05),and those changes did not normalized until re-perfusion 60 min.The 48 h fasting increased significantly endothelium-dependent relaxation of aortic rings response to low concentration acetylcholine (P ＜ 0.05),but did not affect the maximum relaxation of aortic rings response to high concentration acetylcholine and contraction of aortic rings responses to potassium chloride and phenylephrine.The 48 h fasting also enhanced significantly titanic tension of soleus compared to the control [(7.01 ± 1.22) N/cm2 vs (5.5 ± 0.75) N/cm2 for48 h-Fasting vs Control,P ＜0.05].Conclusions The 24 ～48 h fasting may induce inhibition of cardiac function,while fasting 48 h can increase the sensitivity of aortic rings responses to ACh and contraction function of soleus in healthy and male rats.
