中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
10期
1349-1352,1357
,共5页
周晓东%郑华%郑瑞国%张慧芳%唐宁宁%周建大%胡丰
週曉東%鄭華%鄭瑞國%張慧芳%唐寧寧%週建大%鬍豐
주효동%정화%정서국%장혜방%당저저%주건대%호봉
表皮生长因子/治疗应用%银/治疗应用%迟效制剂%糖尿病%伤口愈合/药物作用
錶皮生長因子/治療應用%銀/治療應用%遲效製劑%糖尿病%傷口愈閤/藥物作用
표피생장인자/치료응용%은/치료응용%지효제제%당뇨병%상구유합/약물작용
Epidermal growth factor/therapeutic use%Silver/therapeutic use%Delayed-action preparations%Diabetes mellitus%Wound healing/drug effects
目的 探讨纳米银-表皮生长因子缓释复合体的抗菌效果和治疗大鼠糖尿病难愈性创面的疗效.方法 依照前期方法制备出纳米银-表皮生长因子缓释复合体,设计纳米银-表皮生长因子缓释复合体组、单纯纳米银组、单纯生长因子组、单纯生理盐水对照组、青霉素钠阳性对照组,对比检测各组对5种常见微生物(金黄色葡萄球、大肠埃希菌、铜绿假单胞菌、白色念珠菌、肺炎克雷伯菌)的抗菌活性;然后将糖尿病大鼠创面模型随机分为五组,分别以纳米银-生长因子缓释复合体、纳米银及表皮生长因子联合应用、单纯表皮生长因子、单纯纳米银、生理盐水治疗创面,不同时相观察各组创面愈合情况.结果 缓释复合体及纳米银抗菌性能明显高于其它三组,同时缓释复合体能显著缩短糖尿病大鼠创面愈合时间(P<0.05);第3天各组愈合率差异无统计学意义(P>0.05),第7天和13天缓释复合体的愈合率[(61.71±8.78)%、(100±2.60)%]明显高于其他四组(P<0.05).结论 纳米银-表皮生长因子缓释复合体不仅具有良好的抗菌性,且能快速促进糖尿病创面的愈合.
目的 探討納米銀-錶皮生長因子緩釋複閤體的抗菌效果和治療大鼠糖尿病難愈性創麵的療效.方法 依照前期方法製備齣納米銀-錶皮生長因子緩釋複閤體,設計納米銀-錶皮生長因子緩釋複閤體組、單純納米銀組、單純生長因子組、單純生理鹽水對照組、青黴素鈉暘性對照組,對比檢測各組對5種常見微生物(金黃色葡萄毬、大腸埃希菌、銅綠假單胞菌、白色唸珠菌、肺炎剋雷伯菌)的抗菌活性;然後將糖尿病大鼠創麵模型隨機分為五組,分彆以納米銀-生長因子緩釋複閤體、納米銀及錶皮生長因子聯閤應用、單純錶皮生長因子、單純納米銀、生理鹽水治療創麵,不同時相觀察各組創麵愈閤情況.結果 緩釋複閤體及納米銀抗菌性能明顯高于其它三組,同時緩釋複閤體能顯著縮短糖尿病大鼠創麵愈閤時間(P<0.05);第3天各組愈閤率差異無統計學意義(P>0.05),第7天和13天緩釋複閤體的愈閤率[(61.71±8.78)%、(100±2.60)%]明顯高于其他四組(P<0.05).結論 納米銀-錶皮生長因子緩釋複閤體不僅具有良好的抗菌性,且能快速促進糖尿病創麵的愈閤.
목적 탐토납미은-표피생장인자완석복합체적항균효과화치료대서당뇨병난유성창면적료효.방법 의조전기방법제비출납미은-표피생장인자완석복합체,설계납미은-표피생장인자완석복합체조、단순납미은조、단순생장인자조、단순생리염수대조조、청매소납양성대조조,대비검측각조대5충상견미생물(금황색포도구、대장애희균、동록가단포균、백색념주균、폐염극뢰백균)적항균활성;연후장당뇨병대서창면모형수궤분위오조,분별이납미은-생장인자완석복합체、납미은급표피생장인자연합응용、단순표피생장인자、단순납미은、생리염수치료창면,불동시상관찰각조창면유합정황.결과 완석복합체급납미은항균성능명현고우기타삼조,동시완석복합체능현저축단당뇨병대서창면유합시간(P<0.05);제3천각조유합솔차이무통계학의의(P>0.05),제7천화13천완석복합체적유합솔[(61.71±8.78)%、(100±2.60)%]명현고우기타사조(P<0.05).결론 납미은-표피생장인자완석복합체불부구유량호적항균성,차능쾌속촉진당뇨병창면적유합.
Objective To investigate the antibacterial properties and curative effects of a nanosilver-epidermal growth factor (NanoAg-EGF) sustained-release carrier.Methods First,in accordance with former method,sustained-release carrier of NanoAgEGF was prepared,with the design of the NanoAg-EGF group,NanoAg-alone group,EGF-alone group,control groups of normal saline-alone,and benzylpenicillin sodium.Contrasting detection on antibacterial properties of five kinds of common microbes (staphylococcus aureus,escherichia coli,pseudomonas aeruginosa,candida albicans,and streptococcus pneumonia) in each group was used to confirm their antibacterial properties.Then,wound models of diabetic rats were randomly divided into five groups,with the wound treatments by sustained-release cartier of NanoAg-EGF,combined application of NanoAg + EGF,EGF-alone,NanoAg-alone,and normal saline-alone control group,respectively.The observation on the healing situation of each group in different times was used to ensure the curative effect.Results In the antibacterial experiments,it was found that the NanoAg-EGF and NanoAg had strong antibacterial properties.Their antibacterial properties had no significant difference (P > 0.05).The control group,penicillin sodium only had relatively weak antibacterial properties on staphylococcus aureus and streptococcus pneumoniae,with obviously weaker function compared to the NanoAg-EGF and NanoAg groups (P < 0.05).The control groups,EGF-alone and normal saline-alone had no inhibitor function on each kind of pathogenic microorganisms.In the wound healing experiments on diabetic rats,it was found that NanoAgEGF shortened significantly the wound healing time of diabetic rats (P < 0.05).On the 3rd day,the healing rate of each group had no difference (P > 0.05).On the 7th day and 13th day,the healing rates of NanoAg-EGF [(61.71 ± 8.78)% and (100 ± 2.60) %] were significantly higher than the other four groups (P < 0.05).Conclusions NanoAg-EGF not only has good antibacterial properties but also can promote rapidly the wound healing of diabetes.