CAJ | 학술논문

目的探讨Lewis y抗原及黏蛋白1(MUC1)在卵巢上皮性肿瘤中的表达及意义,并探讨Lewis y抗原与MUC1表达的相关性.方法应用免疫组化SP法检测恶性卵巢上皮性肿瘤60例(其中高分化21例、中分化21例、低分化18例),交界性卵巢上皮性肿瘤30例,良性卵巢上皮性肿瘤30例及正常卵巢组织20例中Lewis y抗原与MUC1的表达情况,并分析两者之间的关系及其与卵巢癌生物学特性的关系.同时利用免疫荧光双标记法检测Lewis y抗原与MUC1在卵巢癌组织中的结构关系.结果在恶性卵巢上皮性肿瘤中,Lewis y抗原的阳性表达率为88.33％,明显高于交界性组(60.00％,x2=9.6405)、良性组(33.33％,x2=28.8095)及正常卵巢组(0,x2=52.3457)(P均＜0.01)；Lewis y抗原的阳性表达率与卵巢癌临床病理参数无关,但其表达强度随恶性程度的增加而增加(P＜0.05).MUC1在卵巢恶性组的阳性表达率为86.67％,明显高于交界性组(53.33％,x2=12.0321)、良性组(30.00％,x2=29.4064)以及正常卵巢组(25.00％,x2=27.8464)(P均＜0.01),其表达强度随临床分期的增加而增加(P＜0.01),与淋巴结转移、组织学分级无关(P均＞0.05)；Lewis y抗原和MUC1在卵巢癌组织中同时高表达,其表达具有正相关(r=0.707,P＜0.01),且二者存在共定位关系.结论 Lewis y抗原及MUC1参与了卵巢癌的发生、发展,可作为判断卵巢癌生物学行为的参考指标,为卵巢癌的生物学治疗提供理论依据.
목적 탐토Lewis y항원급점단백1(MUC1)재란소상피성종류중적표체급의의,병탐토Lewis y항원여MUC1표체적상관성.방법 응용면역조화SP법검측악성란소상피성종류60례(기중고분화21례、중분화21례、저분화18례),교계성란소상피성종류30례,량성란소상피성종류30례급정상란소조직20례중Lewis y항원여MUC1적표체정황,병분석량자지간적관계급기여란소암생물학특성적관계.동시이용면역형광쌍표기법검측Lewis y항원여MUC1재란소암조직중적결구관계.결과 재악성란소상피성종류중,Lewis y항원적양성표체솔위88.33％,명현고우교계성조(60.00％,x2=9.6405)、량성조(33.33％,x2=28.8095)급정상란소조(0,x2=52.3457)(P균＜0.01)；Lewis y항원적양성표체솔여란소암림상병리삼수무관,단기표체강도수악성정도적증가이증가(P＜0.05).MUC1재란소악성조적양성표체솔위86.67％,명현고우교계성조(53.33％,x2=12.0321)、량성조(30.00％,x2=29.4064)이급정상란소조(25.00％,x2=27.8464)(P균＜0.01),기표체강도수림상분기적증가이증가(P＜0.01),여림파결전이、조직학분급무관(P균＞0.05)；Lewis y항원화MUC1재란소암조직중동시고표체,기표체구유정상관(r=0.707,P＜0.01),차이자존재공정위관계.결론 Lewis y항원급MUC1삼여료란소암적발생、발전,가작위판단란소암생물학행위적삼고지표,위란소암적생물학치료제공이론의거.
Objective To investigate the expression and the clinical significance of Lewis y antigen and Mucin 1 (MUC1),as well as to evaluate the correlation between them in epithelial ovarian tumor.Methods The expression of Lewis y antigen and MUC1 in 60 cases of epithelial ovarian malignant tumors,30 cases of borderline ovarian tumors,30 cases of benign ovarian tumors and 20 cases of normal ovarian tissues were detected by immunohistochemical staining.The relationship between Lewis y antigen and MUC1,and their relationship with biology characteristic of ovarian carcinoma were analyzed.An immunofluorescence double labeling methods was performed to detect the correlation between Lewis y antigen and MUC1.Results In malignant epithelial ovarian tumors,the positive rates of Lewis y antigen was 88.33％,which was significantly higher than the positive rates in borderline(60.00％,x2 =9.6405,P ＜0.01) and benign ovarian tumors(33.33％,x2 =28.8095,P ＜0.01) and normal ovarian samples (0,x2 =52.3457,P ＜ 0.01).The positive rates of Lewis y antigen had nothing to do with the clinical pathological parameters of ovarian tumor,but the expression intensity of Lewis yantigen was increased with the development of the malignant degree(P ＜ 0.05).The positive rates of MUC1 in malignant epithelial ovarian tumors was also significantly higher than that in borderline,benign ovarian tumors and normal ovarian samples (86.67％ vs 53.33％,30.00％,25.00％,x2 =12.0321,29.4064,27.8464 ; P ＜0.01).And the expression intensity of MUC1 also increased with the development of clinical stage(P ＜0.01),but had nothing to do with the lymph node metastasis and histological grade(P ＞ 0.05).In ovarian cancer,both Lewis y antigen and MUC1 were highly expressed,and their expression levels were positively correlated (r =0.707,P ＜0.01),and Lewis y antigen colocalized with MUC1.Conclusion Both Lewis y antigen and MUC1 are associated with the occurrence and development of ovarian cancer.Lewis y antigen and MUC1 might be a sigh of biological behavior in ovarian cancers,and this study provides theoretical evidence of ovarian cancer biological treatment.