中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
3期
297-299
,共3页
唐来坤%屈维龙%田峰%汪祖林%宋立%俞仲伟%王珂%吴凤金%羊继平
唐來坤%屈維龍%田峰%汪祖林%宋立%俞仲偉%王珂%吳鳳金%羊繼平
당래곤%굴유룡%전봉%왕조림%송립%유중위%왕가%오봉금%양계평
膀胱癌%去整合素金属蛋白酶12%增殖细胞核抗原%免疫组织化学法
膀胱癌%去整閤素金屬蛋白酶12%增殖細胞覈抗原%免疫組織化學法
방광암%거정합소금속단백매12%증식세포핵항원%면역조직화학법
Bladder carcinoma%A disintegrin and metalloproteinase-12%Proliferating cell nuclear antigen%Immunohistochemistry
目的 探讨去整合素金属蛋白酶12(ADAM-12)和增殖细胞核抗原(PCNA)在膀胱癌中的表达与膀胱癌病理学特点.方法 应用免疫组织化学法检测43例膀胱癌、12例正常膀胱组织中ADAM-12及PCNA的表达情况,并分析其与膀胱癌患者的临床特征及病理分期的关系.结果 免疫染色标本中,ADAM-12在膀胱癌中的表达明显高于正常膀胱组织,两者比较差异有统计学意义(Z =4.879,P＜0.05).组织学分化低的表达明显高于中、高分化者(x2=22.3685,P＜0.01).PCNA在膀胱癌中的表达明显高于正常膀胱组织,两者比较差异有统计学意义(Z =4.879,P＜0.05).组织学分化低的表达明显高于中高分化者(x2=10.665,P=0.0137).ADAM-12与PCNA在膀胱癌中的表达呈正相关(r=1.000,P＜0.0001).结论 膀胱癌组织中PCNA和ADAM-12蛋白呈过度表达,可能与肿瘤的发生、发展密切相关；ADAM-12是膀胱癌具有潜在价值的肿瘤标记物,可以成为新的一项临床观察指标,因此具有临床应用价值.
目的 探討去整閤素金屬蛋白酶12(ADAM-12)和增殖細胞覈抗原(PCNA)在膀胱癌中的錶達與膀胱癌病理學特點.方法 應用免疫組織化學法檢測43例膀胱癌、12例正常膀胱組織中ADAM-12及PCNA的錶達情況,併分析其與膀胱癌患者的臨床特徵及病理分期的關繫.結果 免疫染色標本中,ADAM-12在膀胱癌中的錶達明顯高于正常膀胱組織,兩者比較差異有統計學意義(Z =4.879,P＜0.05).組織學分化低的錶達明顯高于中、高分化者(x2=22.3685,P＜0.01).PCNA在膀胱癌中的錶達明顯高于正常膀胱組織,兩者比較差異有統計學意義(Z =4.879,P＜0.05).組織學分化低的錶達明顯高于中高分化者(x2=10.665,P=0.0137).ADAM-12與PCNA在膀胱癌中的錶達呈正相關(r=1.000,P＜0.0001).結論 膀胱癌組織中PCNA和ADAM-12蛋白呈過度錶達,可能與腫瘤的髮生、髮展密切相關；ADAM-12是膀胱癌具有潛在價值的腫瘤標記物,可以成為新的一項臨床觀察指標,因此具有臨床應用價值.
목적 탐토거정합소금속단백매12(ADAM-12)화증식세포핵항원(PCNA)재방광암중적표체여방광암병이학특점.방법 응용면역조직화학법검측43례방광암、12례정상방광조직중ADAM-12급PCNA적표체정황,병분석기여방광암환자적림상특정급병리분기적관계.결과 면역염색표본중,ADAM-12재방광암중적표체명현고우정상방광조직,량자비교차이유통계학의의(Z =4.879,P＜0.05).조직학분화저적표체명현고우중、고분화자(x2=22.3685,P＜0.01).PCNA재방광암중적표체명현고우정상방광조직,량자비교차이유통계학의의(Z =4.879,P＜0.05).조직학분화저적표체명현고우중고분화자(x2=10.665,P=0.0137).ADAM-12여PCNA재방광암중적표체정정상관(r=1.000,P＜0.0001).결론 방광암조직중PCNA화ADAM-12단백정과도표체,가능여종류적발생、발전밀절상관；ADAM-12시방광암구유잠재개치적종류표기물,가이성위신적일항림상관찰지표,인차구유림상응용개치.
Objective To investigate the expression of a dismtegnn and metalloproteinase-12 (ADAM12) and proliferating cell nuclear antigen (PCNA) in human bladder carcinoma,and to explore their correlation with different grades and stages of bladder cancer.Methods Biopsies of 12 normal bladder and 43 bladder tumors were performed.And immunohistochemistry was conducted to detect the expression of ADAM12 and PCNA in the biopsies.Results Positive expression signals of ADAM12 were detected significantly higher inthe bladder cancer biopsies than that in the normal ones (Z =4.879,P ＜ 0.05),and the expression level of ADAM-12 in lower histological grade was significantly higher than that in the moderate and higher histological grades (x2 =22.3685,P ＜ 0.01).Positive expression signals of PCNA were detected significantly higher in the bladder cancer biopsies than that in the normal ones (Z =4.879,P ＜ 0.05)).Those with lower histological grade had a higher expression level of PCNA when compared with the moderate and higher histological grades (x2 =10.665,P =0.0137).The expression of ADAM-12 was positively correlated with PCNA in bladder cancer (r =1.000,P ＜ 0.0001).Conclusion The over expression of ADAM12 and PCNA maybe play an important role in development of the bladder tumors.And ADAM12 may be a promising biomarker of bladder cancer in the clinical behavior.
