中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
11期
1147-1150
,共4页
刘伟%纪霞%张为忠%王凤华%张伟毅
劉偉%紀霞%張為忠%王鳳華%張偉毅
류위%기하%장위충%왕봉화%장위의
血清瘦素%瘦素受体基因%多态性%哮喘%代谢综合征
血清瘦素%瘦素受體基因%多態性%哮喘%代謝綜閤徵
혈청수소%수소수체기인%다태성%효천%대사종합정
Serum leptin%Leptin receptor gene%Polymorphisms%Asthma%Metabolic syndrome
目的 探讨瘦素受体基因Gln223Arg位点不同基因表型和血清瘦素水平与哮喘的相关性.方法 采用病例对照法,从哮喘基因文库中选择哮喘患者192例,其中单纯哮喘患者100例(单纯哮喘组),哮喘合并代谢综合征92例(哮喘合并代谢综合征组),健康对照者108名(对照组).采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测瘦素受体Gln223Arg基因多态性,用ELISA法测血清瘦素浓度,分析其与哮喘的关系.结果 单纯哮喘组、哮喘合并代谢综合症组和对照组GG、GA和AA基因型频率分别为83.00％ (83/100)、15.00％ (15/100)、2.00％(2/100),83.70％ (77/92)、15.22％(14/92)、1.08％ (1/92)和70.37％ (76/108)、27.78％ (30/108)、1.85％ (2/108)；单纯哮喘组、哮喘合并代谢综合症组和对照组G和A等位基因分别为90.50％、9.50％,91.30％、8.70％和84.26％、15.74％.与对照组比较,单纯哮喘组和哮喘合并代谢综合征组的GG基因型和GA+ AA基因型均存在差异,其中GG基因型患哮喘的风险较高,分别为GA+ AA基因型的2.056和2.161倍(x2=4.599,P=0.032,OR=2.056,95％CI为1.057 ～ 3.999；x2=4.907,P=0.027,OR =2.161,95 ％CI为1.084 ～4.311).单纯哮喘组中,女性血清瘦素水平与对照组间差异有统计学意义(P =0.037,P=2.930)；哮喘合并代谢综合征组男、女血清瘦素水平与对照组比较差异均有统计学意义(P =0.001、0.000,P=4.530、4.690).结论 瘦素受体基因Gln223Arg该位点的多态性与哮喘相关,GG基因型患哮喘的风险较高.血清瘦素水平与女性哮喘呈正相关,尤其与哮喘合并代谢综合征显著相关.
目的 探討瘦素受體基因Gln223Arg位點不同基因錶型和血清瘦素水平與哮喘的相關性.方法 採用病例對照法,從哮喘基因文庫中選擇哮喘患者192例,其中單純哮喘患者100例(單純哮喘組),哮喘閤併代謝綜閤徵92例(哮喘閤併代謝綜閤徵組),健康對照者108名(對照組).採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法檢測瘦素受體Gln223Arg基因多態性,用ELISA法測血清瘦素濃度,分析其與哮喘的關繫.結果 單純哮喘組、哮喘閤併代謝綜閤癥組和對照組GG、GA和AA基因型頻率分彆為83.00％ (83/100)、15.00％ (15/100)、2.00％(2/100),83.70％ (77/92)、15.22％(14/92)、1.08％ (1/92)和70.37％ (76/108)、27.78％ (30/108)、1.85％ (2/108)；單純哮喘組、哮喘閤併代謝綜閤癥組和對照組G和A等位基因分彆為90.50％、9.50％,91.30％、8.70％和84.26％、15.74％.與對照組比較,單純哮喘組和哮喘閤併代謝綜閤徵組的GG基因型和GA+ AA基因型均存在差異,其中GG基因型患哮喘的風險較高,分彆為GA+ AA基因型的2.056和2.161倍(x2=4.599,P=0.032,OR=2.056,95％CI為1.057 ～ 3.999；x2=4.907,P=0.027,OR =2.161,95 ％CI為1.084 ～4.311).單純哮喘組中,女性血清瘦素水平與對照組間差異有統計學意義(P =0.037,P=2.930)；哮喘閤併代謝綜閤徵組男、女血清瘦素水平與對照組比較差異均有統計學意義(P =0.001、0.000,P=4.530、4.690).結論 瘦素受體基因Gln223Arg該位點的多態性與哮喘相關,GG基因型患哮喘的風險較高.血清瘦素水平與女性哮喘呈正相關,尤其與哮喘閤併代謝綜閤徵顯著相關.
목적 탐토수소수체기인Gln223Arg위점불동기인표형화혈청수소수평여효천적상관성.방법 채용병례대조법,종효천기인문고중선택효천환자192례,기중단순효천환자100례(단순효천조),효천합병대사종합정92례(효천합병대사종합정조),건강대조자108명(대조조).채용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측수소수체Gln223Arg기인다태성,용ELISA법측혈청수소농도,분석기여효천적관계.결과 단순효천조、효천합병대사종합증조화대조조GG、GA화AA기인형빈솔분별위83.00％ (83/100)、15.00％ (15/100)、2.00％(2/100),83.70％ (77/92)、15.22％(14/92)、1.08％ (1/92)화70.37％ (76/108)、27.78％ (30/108)、1.85％ (2/108)；단순효천조、효천합병대사종합증조화대조조G화A등위기인분별위90.50％、9.50％,91.30％、8.70％화84.26％、15.74％.여대조조비교,단순효천조화효천합병대사종합정조적GG기인형화GA+ AA기인형균존재차이,기중GG기인형환효천적풍험교고,분별위GA+ AA기인형적2.056화2.161배(x2=4.599,P=0.032,OR=2.056,95％CI위1.057 ～ 3.999；x2=4.907,P=0.027,OR =2.161,95 ％CI위1.084 ～4.311).단순효천조중,녀성혈청수소수평여대조조간차이유통계학의의(P =0.037,P=2.930)；효천합병대사종합정조남、녀혈청수소수평여대조조비교차이균유통계학의의(P =0.001、0.000,P=4.530、4.690).결론 수소수체기인Gln223Arg해위점적다태성여효천상관,GG기인형환효천적풍험교고.혈청수소수평여녀성효천정정상관,우기여효천합병대사종합정현저상관.
Objective To investigate the relationship between leptin receptor(LEPR) gene Gln223Arg in polymorphism and serum leptin level of patients with asthma.Methods A case-control study was conducted.One hundred and ninety-two asthma patients were recruited from asthma gene library,among them 100 patients only with asthma(asthma group) and 92 patients with both asthma and metabolic syndrome(asthma complicated with metabolic syndrome group).And 108 normal people were selected as control group.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of LEPR gene Gln223Arg locus.Serum leptin levels were measured by enzyme-linked immuno sorbent assay,its relationship with asthma was analyze.Results The frequencies of genotype GG,GA,AA in asthma group,asthma complicated with metabolic syndrome group and normal control group were (83.00％ (83/100) vs.83.70％ (77/92) vs.70.37％ (76/108),15.00％ (15/100) vs.15.22％ (14/92) vs.27.78％ (30/108),and2.00％ (2/100) vs.1.08％ (1/92) vs.1.85％ (2/108) respectively).G and A alleles in asthma group,asthma complicated with metabolic syndrome group and normal control group were 90.50％ vs.91.30％vs.84.26％,9.50％ vs.8.70％ vs.15.74％ respectively.Compared with the control group,GG genotype and GA + AA genotype were different between in asthma group and asthma complicated with metabolic syndrome group.The patients with GG genotype showed 2.056 (x2 =4.599,P =0.032,OR =2.056,95％ CI =1.057-3.999) and 2.161 folds (x2 =4.907,P =0.027,OR =2.161,95 ％ CI =1.084-4.311) risk in asthma group and asthma complicated with metabolic syndrome group than that with GA + AA genotype.In terms of leptin levels,in asthma group,female were significant higher than that of control group (P =0.037,P =2.93),while in asthma complicated with metabolic syndrome group,male and female were significantly higher than control group (P =0.001,0.000 ; P =4.530,4.690).Conclusion The polymorphisms of LEPR gene locus Gln223 Arg are related with asthma.Genotype GG carrier is susceptible for the disease.In female,serum leptin is positive relationship with asthma group,especially in asthma complicated with metabolic syndrome.
