CAJ | 학술논문

目的探讨白细胞介素1β(IL-1β)-31T/C和-511C/T与慢性阻塞性肺疾病(COPD)的相关性.方法收集2009年1月至2012年6月河北联合大学附属开滦总医院呼吸内科住院治疗的COPD患者(COPD组)及260名同期健康对照人群(对照组).用酶联免疫吸附试验检测血清IL-1β浓度,应用聚合酶链反应-限制性片段长度多态性方法检测-31T/C和-511C/T位点的基因型.结果 COPD组血清IL-1β为(3.92±0.42) μg/L,对照组为(2.69 ±0.11) μg/L,组间比较差异有统计学意义(t=12.889,P＜0.001).COPD组IL-1β-31的TT、CT和CC基因型频率分别为20.8％(54/260),58.1％ (151/260)和21.2％ (55/260),对照组分别为19.2％ (50/260),58.8％ (153/260)和21.9％ (57/260),两组相比差异无统计学意义(x2=0.203,P=0.904).COPD组IL-1β-511的CC、CT和TT基因型频率分别为20.0％(52/260),63.1％ (164/260)和16.9％ (44/260),对照组分别为21.9％ (57/260)、60.8％ (158/260)和17.3％ (45/260),两组相比差异无统计学意义(x2=0.352,P=0.838).并且,男性和女性人群亚组分析中两组人群差异均无统计学意义(P均＞0.05).结论 COPD组血清IL-1β水平显著高于对照组.IL-1β中-31,-511的基因型频率与等位基因频率与COPD的发生无明显相关.
목적 탐토백세포개소1β(IL-1β)-31T/C화-511C/T여만성조새성폐질병(COPD)적상관성.방법 수집2009년1월지2012년6월하북연합대학부속개란총의원호흡내과주원치료적COPD환자(COPD조)급260명동기건강대조인군(대조조).용매련면역흡부시험검측혈청IL-1β농도,응용취합매련반응-한제성편단장도다태성방법검측-31T/C화-511C/T위점적기인형.결과 COPD조혈청IL-1β위(3.92±0.42) μg/L,대조조위(2.69 ±0.11) μg/L,조간비교차이유통계학의의(t=12.889,P＜0.001).COPD조IL-1β-31적TT、CT화CC기인형빈솔분별위20.8％(54/260),58.1％ (151/260)화21.2％ (55/260),대조조분별위19.2％ (50/260),58.8％ (153/260)화21.9％ (57/260),량조상비차이무통계학의의(x2=0.203,P=0.904).COPD조IL-1β-511적CC、CT화TT기인형빈솔분별위20.0％(52/260),63.1％ (164/260)화16.9％ (44/260),대조조분별위21.9％ (57/260)、60.8％ (158/260)화17.3％ (45/260),량조상비차이무통계학의의(x2=0.352,P=0.838).병차,남성화녀성인군아조분석중량조인군차이균무통계학의의(P균＞0.05).결론 COPD조혈청IL-1β수평현저고우대조조.IL-1β중-31,-511적기인형빈솔여등위기인빈솔여COPD적발생무명현상관.
Objective To investigate the relationship between polymorphism of interleukin-1β(IL-1β)-31,-511C/T and chronic obstructive pulmonary disease.Methods Two hundred and sixty patients with chronic obstructive pulmonary disease (COPD) were selected as our subjects who were hospitalized in General Hospital of Kailuan from January 2009 to June 2012.At same time,the 260 healthy controls were recruited in medical examine center.The data was collected by the physical examination and a unified questionnaire.Enzyme-linked immunosorbent assay (ELISA) was used to measure the level of IL-1β in serum.Polymerize chain reaction-Restriction fragment length polymorphism (PCR-RLFP) was used to detect the genotypes of IL-1β (-31,-511).Multivariate logistic regression was used to analyze the relations between risk factors with susceptibility of COPD.Results The level of serum IL-1β was (3.92 ± 0.42) μg/L in COPD group,higher than that in control group ((2.69 ± 0.11) μg/L,t =12.889,P ＜ 0.001).The frequency of genotype of IL-1β-31 site in COPD group were 20.8％ (54/260) for TT,58.1％ (151/260) for CT and 21.2％ (55/260) for CC respectively.Meanwhile IL-1β-31 genotype rate were 19.2％ (50/260),58.8％ (153/260) and 21.9％ (57/260) respectively in control group and no significant difference was found between two groups (x2 =0.203,P =0.904).The frequency of genotype of IL-1β-511 site were 20.0％ (52/260) for CC,63.1％ (164/260) for CT and 16.9％ (44/260) for TT in COPD group.The three genotype rate in control group were 21.9％ (57/260),60.8％ (158/260) and 17.3％ (45/260) respectively,and no significant difference was found between two groups (x2 =0.352,P =0.838).Moreover there was also no significant difference in terms of gender(P ＞ 0.05)Conclusion The concentration of IL-1β in serum in COPD group was higher than in control group.The polymorphism of-31 and-511 were proved non association with COPD.