CAJ | 학술논문

目的观察血小板源性生长因子(PDGF)在小肠移植慢性排斥反应中的表达.方法利用显微外科技术构建同系和异系大鼠小肠移植模型,以同系移植作为对照组,异系移植作为实验组.分别在术后第7、28、90天进行移植物活检,采用免疫组织化学方法对PDGF因子的表达进行定位.应用Realtime-PCR技术检测移植物内PDGF因子mRNA转录水平.结果实验终末期小肠移植物呈现组织纤维化等慢性排斥反应的特有表现,免疫组化显示PDGF因子在间质及小肠腺体内高表达.所有移植物中PDGF因子mRNA转录水平呈逐渐上升趋势,而POD90所采集的标本中以实验组的mRNA转录水平最高.结论 PDGF在小肠移植后期明显高表达,对于小肠移植慢性排斥反应的发生具有重要意义.
목적 관찰혈소판원성생장인자(PDGF)재소장이식만성배척반응중적표체.방법 이용현미외과기술구건동계화이계대서소장이식모형,이동계이식작위대조조,이계이식작위실험조.분별재술후제7、28、90천진행이식물활검,채용면역조직화학방법대PDGF인자적표체진행정위.응용Realtime-PCR기술검측이식물내PDGF인자mRNA전록수평.결과 실험종말기소장이식물정현조직섬유화등만성배척반응적특유표현,면역조화현시PDGF인자재간질급소장선체내고표체.소유이식물중PDGF인자mRNA전록수평정축점상승추세,이POD90소채집적표본중이실험조적mRNA전록수평최고.결론 PDGF재소장이식후기명현고표체,대우소장이식만성배척반응적발생구유중요의의.
Objective To investigate the expression of platelet derived growth factor(PDGF) in small bowel transplantation of rats.Methods Isogeneic and allogeneic small bowel transplantation were performed in rats by microsurgical technology.All rats were divided into two groups:isogeneic control group and allogeneic test group.Transplanted tissues were test on 7th,28th and 90th after surgery.Positioning using immunohistochemical method the expression of PDGF.Real time PCR and immunohistochemical staining were also performed to detect the expression of PDGF.Results The unique feature including intestinal graft fibrosis was showed in tissues.Immunohistochemistry results showed PDGF expression was higher in intestinal glands.PDGF mRNA levels in transplanted tissues showed a gradual upward trend,and the top levels is in POD90.Conclusion PDGF expression was significantly higher in the late of intestinal transplantation,which showed an guideline for chronic rejection of intestinal transplantation.