中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
z1期
23-25
,共3页
p120-catenin%T24细胞%增殖%侵袭
p120-catenin%T24細胞%增殖%侵襲
p120-catenin%T24세포%증식%침습
p120ctn%T24 cells%Proliferation%Invasion
目的 研究p120-catenin (p120ctn)对膀胱癌细胞增殖和侵袭是否有调节作用,探讨p120ctn下调对膀胱癌T24细胞增殖和侵袭能力的影响.方法 应用siRNA方法沉默T24细胞中p120ctn基因表达,采用MTT法检测p120ctn-siRNA对T24细胞生长率的影响、transwell法检测T24细胞侵袭能力的变化.结果 p120ctn-siRNA能够促进T24细胞增殖.transwell法检测p120ctn-siRNA处理后的T24体外侵袭能力明显升高.结论 p120ctn抑制膀胱癌T24细胞的增殖,同时降低T24细胞侵袭能力.
目的 研究p120-catenin (p120ctn)對膀胱癌細胞增殖和侵襲是否有調節作用,探討p120ctn下調對膀胱癌T24細胞增殖和侵襲能力的影響.方法 應用siRNA方法沉默T24細胞中p120ctn基因錶達,採用MTT法檢測p120ctn-siRNA對T24細胞生長率的影響、transwell法檢測T24細胞侵襲能力的變化.結果 p120ctn-siRNA能夠促進T24細胞增殖.transwell法檢測p120ctn-siRNA處理後的T24體外侵襲能力明顯升高.結論 p120ctn抑製膀胱癌T24細胞的增殖,同時降低T24細胞侵襲能力.
목적 연구p120-catenin (p120ctn)대방광암세포증식화침습시부유조절작용,탐토p120ctn하조대방광암T24세포증식화침습능력적영향.방법 응용siRNA방법침묵T24세포중p120ctn기인표체,채용MTT법검측p120ctn-siRNA대T24세포생장솔적영향、transwell법검측T24세포침습능력적변화.결과 p120ctn-siRNA능구촉진T24세포증식.transwell법검측p120ctn-siRNA처리후적T24체외침습능력명현승고.결론 p120ctn억제방광암T24세포적증식,동시강저T24세포침습능력.
Objective To investigate the regulating function of p120-catenin (p120ctn) on proliferation and invasion of human bladder cancer cell T24.Methods Special siRNA was used to silence the expression of p120ctn in T24 cells.MTT assay was used to examine the T24 cell growth rate.The invasion of T24 cells and the control cells were measured by transwell chamber assay.Results The silencing of p120ctn could improve the proliferation and the invasiveness of T24 tumor cells.Transwell chamber assay showed that the in vitro invasion function of T24 cells was significant increase after p120ctn-siRNA.Conclusion p120ctn inhibited the bladder cancer proliferation and invasiveness of bladder carcinoma cells.
