中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2014年
8期
817-819
,共3页
郭延霞%陈燕%韩素新%何艳舫
郭延霞%陳燕%韓素新%何豔舫
곽연하%진연%한소신%하염방
足月胎膜早破%锌%基质金属蛋白酶9%Ⅳ型胶原
足月胎膜早破%鋅%基質金屬蛋白酶9%Ⅳ型膠原
족월태막조파%자%기질금속단백매9%Ⅳ형효원
Premature rupture of membranes at term%Zinc%Matrix metalloproteinases-9%Collagen Ⅳ
目的 检测足月胎膜早破孕妇血浆锌、血清及羊水基质金属蛋白酶(MMP)9、血清Ⅳ型胶原含量变化.方法 选择2012年11月至2013年3月确诊为足月胎膜早破患者30例作为胎膜早破组,另30例在同一时期正常分娩的非胎膜早破患者作为对照组,收集所有病例的母体血液及羊水,血浆锌含量用原子吸收法检测,血清和羊水MMP-9含量用ELISA法检测、血清Ⅳ型胶原含量用上转发光法检测,并对它们之间的相关性进行分析.结果 胎膜早破组与对照组比较,孕妇血浆锌、血清Ⅳ型胶原、血清MMP-9、羊水MMP-9比较,差异均有统计学意义[锌:(109.10±16.07) μnol/L与(90.54±10.99)μmol/L,t=-5.22,P=0.000;血清Ⅳ型胶原:(56.86±41.26) μg/L与(88.61±44.87))μg/L,t=2.852,P=0.006;血清MMP-9:(1 463.25±483.6) μg/L与(1 196.9±357.43) μg/L,t=-2.426,P=0.018;羊水MMP-9:(125.48±67.18) μg/L与(72.64±60.74) μg/L,t=-2.873,P=0.006].孕妇血浆锌与血清Ⅳ型胶原之间呈负相关(r=-0.261,P=0.044);孕妇血浆锌、血清MMP-9之间呈正相关(r =0.274,P=0.034);孕妇血清MMP-9、羊水MMP-9之间呈正相关(r=0.264,P=0.047);血浆锌与羊水MMP-9、血清Ⅳ型胶原与血清MMP-9、血清Ⅳ型胶原与羊水MMP-9之间均无相关性(r值分别为0.215、-0.172、-0.172,P均＞0.05).结论 足月胎膜早破与母体血浆锌、血清及羊水MMP-9含量升高、血清Ⅳ型胶原含量降低有关.
目的 檢測足月胎膜早破孕婦血漿鋅、血清及羊水基質金屬蛋白酶(MMP)9、血清Ⅳ型膠原含量變化.方法 選擇2012年11月至2013年3月確診為足月胎膜早破患者30例作為胎膜早破組,另30例在同一時期正常分娩的非胎膜早破患者作為對照組,收集所有病例的母體血液及羊水,血漿鋅含量用原子吸收法檢測,血清和羊水MMP-9含量用ELISA法檢測、血清Ⅳ型膠原含量用上轉髮光法檢測,併對它們之間的相關性進行分析.結果 胎膜早破組與對照組比較,孕婦血漿鋅、血清Ⅳ型膠原、血清MMP-9、羊水MMP-9比較,差異均有統計學意義[鋅:(109.10±16.07) μnol/L與(90.54±10.99)μmol/L,t=-5.22,P=0.000;血清Ⅳ型膠原:(56.86±41.26) μg/L與(88.61±44.87))μg/L,t=2.852,P=0.006;血清MMP-9:(1 463.25±483.6) μg/L與(1 196.9±357.43) μg/L,t=-2.426,P=0.018;羊水MMP-9:(125.48±67.18) μg/L與(72.64±60.74) μg/L,t=-2.873,P=0.006].孕婦血漿鋅與血清Ⅳ型膠原之間呈負相關(r=-0.261,P=0.044);孕婦血漿鋅、血清MMP-9之間呈正相關(r =0.274,P=0.034);孕婦血清MMP-9、羊水MMP-9之間呈正相關(r=0.264,P=0.047);血漿鋅與羊水MMP-9、血清Ⅳ型膠原與血清MMP-9、血清Ⅳ型膠原與羊水MMP-9之間均無相關性(r值分彆為0.215、-0.172、-0.172,P均＞0.05).結論 足月胎膜早破與母體血漿鋅、血清及羊水MMP-9含量升高、血清Ⅳ型膠原含量降低有關.
목적 검측족월태막조파잉부혈장자、혈청급양수기질금속단백매(MMP)9、혈청Ⅳ형효원함량변화.방법 선택2012년11월지2013년3월학진위족월태막조파환자30례작위태막조파조,령30례재동일시기정상분면적비태막조파환자작위대조조,수집소유병례적모체혈액급양수,혈장자함량용원자흡수법검측,혈청화양수MMP-9함량용ELISA법검측、혈청Ⅳ형효원함량용상전발광법검측,병대타문지간적상관성진행분석.결과 태막조파조여대조조비교,잉부혈장자、혈청Ⅳ형효원、혈청MMP-9、양수MMP-9비교,차이균유통계학의의[자:(109.10±16.07) μnol/L여(90.54±10.99)μmol/L,t=-5.22,P=0.000;혈청Ⅳ형효원:(56.86±41.26) μg/L여(88.61±44.87))μg/L,t=2.852,P=0.006;혈청MMP-9:(1 463.25±483.6) μg/L여(1 196.9±357.43) μg/L,t=-2.426,P=0.018;양수MMP-9:(125.48±67.18) μg/L여(72.64±60.74) μg/L,t=-2.873,P=0.006].잉부혈장자여혈청Ⅳ형효원지간정부상관(r=-0.261,P=0.044);잉부혈장자、혈청MMP-9지간정정상관(r =0.274,P=0.034);잉부혈청MMP-9、양수MMP-9지간정정상관(r=0.264,P=0.047);혈장자여양수MMP-9、혈청Ⅳ형효원여혈청MMP-9、혈청Ⅳ형효원여양수MMP-9지간균무상관성(r치분별위0.215、-0.172、-0.172,P균＞0.05).결론 족월태막조파여모체혈장자、혈청급양수MMP-9함량승고、혈청Ⅳ형효원함량강저유관.
Objective To investigate the changes of zinc in maternal plasma and MMP-9,collagen Ⅳ levels in serum and matrix metalloproteinases-9 (MMP-9) level in amniotic fluid in patients with premature rupture of membranes at term(TPROM).Methods Thirty cases who were diagnosed as PROM at term during Nov.2012 to Mar.2013 were enrolled as case group,and 30 cases delivered during the same time without PROM were enrolled as control group.Maternal blood and ammiotic fluid were collected from all the cases.The level of zinc in maternal plasma was measured by atomic absorption method and the levels of MMP-9 in serum and amniotic fluid were detected by enzyme linked immunosorbent assay(ELISA),while the level of collagen Ⅳ in serum was measured by up-conversion luminescence method.The relationship among them was analyzed.Results Compared to control group,there were statistically significant difference between TPROM and control groups in terms of the level of zinc,collagen Ⅳ,MMP-9 in serum and MMP-9 in amniotic fluid (zinc:(109.10 ± 16.07) μmol/L vs.(90.54 ± 10.99) μmol/L; t =-5.22,P ＜ 0.001 ; collagen Ⅳ:(56.86 ±41.26) μg/L vs.(88.61 ±44.87) μg/L;t =2.852,P =0.006;MMP-9 in serum:(1 463.25 ±483.6) μg/L vs.(1 196.9 ± 357.43) μg/L,t =-2.426,P =0.018 ; MMP-9 in amniotic fluid:(125.48 ± 67.18) μg/L vs.(72.64 ± 60.74) μg/L,t =-2.873,P =0.006).Zinc level in maternal plasma and collagen Ⅳ in serum had a negative relationship in TPROM (r =-0.261,P =0.044).Zinc level in maternal plasma and MMP-9 level in serum had a positive relationship in TPROM (r =0.274,P =0.034).MMP-9 levels in serum and amniotic fluid had a positive relationship in TPROM (r =0.264,P =0.047).There were no significant relationship between zinc level in maternal plasma,MMP-9 level in amniotic fluid,collagen Ⅳ and MMP-9 levels in serum,collagen Ⅳ in serum and MMP-9 in amniotic fluid (r =0.215,-0.172,-0.172 ; P ＞ 0.05).Conclusion The level of zinc in maternal plasma and increase of MMP-9 in serum and amniotic fluid of women and decrease of the level of collagen Ⅳ in serum are related to the occurrence of TPROM.
