中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2014年
9期
907-910
,共4页
徐雷%杨召川%孔令朋%李德华%曲政海
徐雷%楊召川%孔令朋%李德華%麯政海
서뢰%양소천%공령붕%리덕화%곡정해
平喘固本合剂%哮喘%气道重塑%血管内皮转化因子%核转录因子κB
平喘固本閤劑%哮喘%氣道重塑%血管內皮轉化因子%覈轉錄因子κB
평천고본합제%효천%기도중소%혈관내피전화인자%핵전록인자κB
Pingchuan-Guben Decoction%Asthma%Airway remodeling%Vascular endothelial growth factor%Nuclear factor-κB
目的 探讨平喘固本合剂对哮喘小鼠的气道慢性炎症及重塑的作用及其相关作用机制.方法 24只昆系小鼠建立哮喘小鼠模型,按随机数字表法分为3组:正常对照组、哮喘模型组、平喘固本合剂治疗组,每组各8只.对各组支气管肺泡灌洗液(BALF)中各种细胞进行分类并计数,肺组织病理切片行HE染色后观察气道重塑情况并检测气道管壁厚度,采用免疫组织化学法检测血管内皮生长因子(VEGF)、核转录因子κB(NF-κB)的表达情况.结果 (1)肺组织形态学变化:HE染色显示平喘固本合剂治疗组较哮喘模型组炎症细胞浸润、平滑肌肥厚及黏膜气道组织水肿、上皮细胞脱落等表现明显减轻.(2)各组小鼠支气管肺泡灌洗液(BALF)中炎性细胞分类计数:哮喘模型组嗜酸性粒细胞计数[(2.15 ±0.44)×108/L]、气管壁厚度[(16.66±1.52) μm2/μm]、气道上皮组织中NF-κB、VEGF表达水平(36.01±4.78、35.87±4.92)明显高于正常对照组嗜酸性粒细胞计数[(0.03 ±0.03)×108/L]、气管壁厚度[(6.61±1.14) μm2/μm]、气道上皮组织中NF-κB、VEGF表达水平(12.78±1.47、11.57±1.64),两组比较差异有统计学意义(P均＜0.01).平喘固本合剂治疗组BALF中嗜酸性粒细胞数[(0.35±0.12)×108/L]、气管壁厚度[(11.57±1.26) μm2/μm]及NF-κB(29.13±1.92)、VEGF (28.28±2.02)明显低于哮喘模型组,两组比较差异有统计学意义(P均＜0.01).结论 平喘固本合剂可以下调哮喘模型小鼠气道组织中NF-κB和VEGF的表达,达到抑制哮喘气道慢性炎症及重塑的作用.
目的 探討平喘固本閤劑對哮喘小鼠的氣道慢性炎癥及重塑的作用及其相關作用機製.方法 24隻昆繫小鼠建立哮喘小鼠模型,按隨機數字錶法分為3組:正常對照組、哮喘模型組、平喘固本閤劑治療組,每組各8隻.對各組支氣管肺泡灌洗液(BALF)中各種細胞進行分類併計數,肺組織病理切片行HE染色後觀察氣道重塑情況併檢測氣道管壁厚度,採用免疫組織化學法檢測血管內皮生長因子(VEGF)、覈轉錄因子κB(NF-κB)的錶達情況.結果 (1)肺組織形態學變化:HE染色顯示平喘固本閤劑治療組較哮喘模型組炎癥細胞浸潤、平滑肌肥厚及黏膜氣道組織水腫、上皮細胞脫落等錶現明顯減輕.(2)各組小鼠支氣管肺泡灌洗液(BALF)中炎性細胞分類計數:哮喘模型組嗜痠性粒細胞計數[(2.15 ±0.44)×108/L]、氣管壁厚度[(16.66±1.52) μm2/μm]、氣道上皮組織中NF-κB、VEGF錶達水平(36.01±4.78、35.87±4.92)明顯高于正常對照組嗜痠性粒細胞計數[(0.03 ±0.03)×108/L]、氣管壁厚度[(6.61±1.14) μm2/μm]、氣道上皮組織中NF-κB、VEGF錶達水平(12.78±1.47、11.57±1.64),兩組比較差異有統計學意義(P均＜0.01).平喘固本閤劑治療組BALF中嗜痠性粒細胞數[(0.35±0.12)×108/L]、氣管壁厚度[(11.57±1.26) μm2/μm]及NF-κB(29.13±1.92)、VEGF (28.28±2.02)明顯低于哮喘模型組,兩組比較差異有統計學意義(P均＜0.01).結論 平喘固本閤劑可以下調哮喘模型小鼠氣道組織中NF-κB和VEGF的錶達,達到抑製哮喘氣道慢性炎癥及重塑的作用.
목적 탐토평천고본합제대효천소서적기도만성염증급중소적작용급기상관작용궤제.방법 24지곤계소서건립효천소서모형,안수궤수자표법분위3조:정상대조조、효천모형조、평천고본합제치료조,매조각8지.대각조지기관폐포관세액(BALF)중각충세포진행분류병계수,폐조직병리절편행HE염색후관찰기도중소정황병검측기도관벽후도,채용면역조직화학법검측혈관내피생장인자(VEGF)、핵전록인자κB(NF-κB)적표체정황.결과 (1)폐조직형태학변화:HE염색현시평천고본합제치료조교효천모형조염증세포침윤、평활기비후급점막기도조직수종、상피세포탈락등표현명현감경.(2)각조소서지기관폐포관세액(BALF)중염성세포분류계수:효천모형조기산성립세포계수[(2.15 ±0.44)×108/L]、기관벽후도[(16.66±1.52) μm2/μm]、기도상피조직중NF-κB、VEGF표체수평(36.01±4.78、35.87±4.92)명현고우정상대조조기산성립세포계수[(0.03 ±0.03)×108/L]、기관벽후도[(6.61±1.14) μm2/μm]、기도상피조직중NF-κB、VEGF표체수평(12.78±1.47、11.57±1.64),량조비교차이유통계학의의(P균＜0.01).평천고본합제치료조BALF중기산성립세포수[(0.35±0.12)×108/L]、기관벽후도[(11.57±1.26) μm2/μm]급NF-κB(29.13±1.92)、VEGF (28.28±2.02)명현저우효천모형조,량조비교차이유통계학의의(P균＜0.01).결론 평천고본합제가이하조효천모형소서기도조직중NF-κB화VEGF적표체,체도억제효천기도만성염증급중소적작용.
Objective To investigate the potential suppression role of Pingchuan-Guben Decoction in airway chronic inflammation and remodeling by observing the expression of vascular endothelial growth factor (VEGF) and NF-κB on the mice asthma model Methods Forty Kunming mice were randomly divided into three groups.Those were negative control group(A),asthma model group(B),Pingchuan-Guben Decoction group (C),and every group was 8 mice.Total and differential cells were counted from bronchoalveolar lavage fluid (BALF).Histological examination(HE) was performed to observe the general pathologic alteration and analyze the thickness of airway wall.The protein expression of VEGF and NF-κB were detected by immunohistochemistry.Results (1)HE staining showed that compared with the Pingchuan-Guben Decoction group,there were a large number of inflammatory cells infiltration,heavier smooth muscle hypertrophy and mucous membrane hyperemia in mice of the asthma group,(2) The Eosinophil cell counting in BALF,airway wall thickness NF-κB,VEGF expression levels in rats asthma model group were (2.15 ± 0.44) × 108/L,(16.66 ± 1.52) μm2/μm,(36.01 ± 4.78),(35.87 ± 4.92) respectively,significantly higher than that in control group ((0.03 ±0.03) × 108/L,(6.61 ± 1.14) μm2/μm,(12.78 ± 1.47),(11.57 ± 1.64) ;P ＜ 0.01).The Eosinophil cell counting in BALF,airway wall thickness NF-κB,VEGF expression levels in Pingchuan-Guben Decoction group were (0.35 ± 0.12) × 108/L,(11.57 ± 1.26) μm2/μm,(29.13 ± 1.92),(28.28 ± 2.02) respectively,significantly higher than that in rats asthma model group (P ＜ 0.01).Conclusion PingchuanGuben Decoction can decrease NF-κB and VEGF expression of asthma mice,and then inhibits airway chronic inflammation and remodeling.
