中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
11期
733-736
,共4页
吴海霞%宋剑婵%师宜荃%刘易欣
吳海霞%宋劍嬋%師宜荃%劉易訢
오해하%송검선%사의전%류역흔
子宫内膜肿瘤%结直肠肿瘤,遗传性非息肉性%DNA错配修复%免疫组织化学
子宮內膜腫瘤%結直腸腫瘤,遺傳性非息肉性%DNA錯配脩複%免疫組織化學
자궁내막종류%결직장종류,유전성비식육성%DNA착배수복%면역조직화학
Endometrial neoplasms%Colorectal neoplasms,hereditary nonpolyposis%DNA mismatch repair%Immunohistochemistry
目的 研究50岁以下中青年子宫内膜癌中DNA错配修复(MMR)基因 MLH1 、MSH2、MSH6和PMS2的表达情况,并分析这4项指标的表达与子宫内膜癌患者的体质量指数(BMI)、肿瘤分级及分期等临床病理特征的关系.方法 50岁以下子宫内膜癌女性作为实验组,50岁以下子宫内膜复杂不典型增生、子宫内膜单纯性增生、正常周期子宫内膜及60岁以上子宫内膜癌病例作为对照组,采用免疫组织化学SP法检测MMR蛋白,分别进行比较,观察上述各项指标在50岁以下女性子宫内膜良恶性病变中的表达规律.结果 40例子宫内膜腺癌患者中有27例(67.5％)至少有一种MMR蛋白表达阴性,在复杂不典型增生、单纯性增生和正常周期子宫内膜组中4项指标至少有一项为阴性的比例分别为5/15、1/13、1/11,实验组与对照组进行比较,差异具有统计学意义(P＜0.01).MLH1、MSH2、MSH6和PMS2蛋白在子宫内膜癌中的阴性表达率分别为52.5％、12.5％ 、35.0％和30.0％；与对照组分别进行比较,MLH1和MSH6的表达差异均有统计学意义(P＜0.05),MSH2的表达各组间差异均无统计学意义(P =0.295).MMR中4项指标的表达与子宫内膜癌的肿瘤分期和组织学分级无关,但MSH6的阴性表达更倾向于发生在高BMI病例中.结论 MMR基因突变与子宫内膜复杂不典型增生及子宫内膜癌的发生有一定的相关性,但在临床病理指标上,除MSH6缺失表达与高BMI相关外,其他4项MMR指标与子宫内膜癌的肿瘤分期和组织学分级无明显相关性.
目的 研究50歲以下中青年子宮內膜癌中DNA錯配脩複(MMR)基因 MLH1 、MSH2、MSH6和PMS2的錶達情況,併分析這4項指標的錶達與子宮內膜癌患者的體質量指數(BMI)、腫瘤分級及分期等臨床病理特徵的關繫.方法 50歲以下子宮內膜癌女性作為實驗組,50歲以下子宮內膜複雜不典型增生、子宮內膜單純性增生、正常週期子宮內膜及60歲以上子宮內膜癌病例作為對照組,採用免疫組織化學SP法檢測MMR蛋白,分彆進行比較,觀察上述各項指標在50歲以下女性子宮內膜良噁性病變中的錶達規律.結果 40例子宮內膜腺癌患者中有27例(67.5％)至少有一種MMR蛋白錶達陰性,在複雜不典型增生、單純性增生和正常週期子宮內膜組中4項指標至少有一項為陰性的比例分彆為5/15、1/13、1/11,實驗組與對照組進行比較,差異具有統計學意義(P＜0.01).MLH1、MSH2、MSH6和PMS2蛋白在子宮內膜癌中的陰性錶達率分彆為52.5％、12.5％ 、35.0％和30.0％；與對照組分彆進行比較,MLH1和MSH6的錶達差異均有統計學意義(P＜0.05),MSH2的錶達各組間差異均無統計學意義(P =0.295).MMR中4項指標的錶達與子宮內膜癌的腫瘤分期和組織學分級無關,但MSH6的陰性錶達更傾嚮于髮生在高BMI病例中.結論 MMR基因突變與子宮內膜複雜不典型增生及子宮內膜癌的髮生有一定的相關性,但在臨床病理指標上,除MSH6缺失錶達與高BMI相關外,其他4項MMR指標與子宮內膜癌的腫瘤分期和組織學分級無明顯相關性.
목적 연구50세이하중청년자궁내막암중DNA착배수복(MMR)기인 MLH1 、MSH2、MSH6화PMS2적표체정황,병분석저4항지표적표체여자궁내막암환자적체질량지수(BMI)、종류분급급분기등림상병리특정적관계.방법 50세이하자궁내막암녀성작위실험조,50세이하자궁내막복잡불전형증생、자궁내막단순성증생、정상주기자궁내막급60세이상자궁내막암병례작위대조조,채용면역조직화학SP법검측MMR단백,분별진행비교,관찰상술각항지표재50세이하녀성자궁내막량악성병변중적표체규률.결과 40례자궁내막선암환자중유27례(67.5％)지소유일충MMR단백표체음성,재복잡불전형증생、단순성증생화정상주기자궁내막조중4항지표지소유일항위음성적비례분별위5/15、1/13、1/11,실험조여대조조진행비교,차이구유통계학의의(P＜0.01).MLH1、MSH2、MSH6화PMS2단백재자궁내막암중적음성표체솔분별위52.5％、12.5％ 、35.0％화30.0％；여대조조분별진행비교,MLH1화MSH6적표체차이균유통계학의의(P＜0.05),MSH2적표체각조간차이균무통계학의의(P =0.295).MMR중4항지표적표체여자궁내막암적종류분기화조직학분급무관,단MSH6적음성표체경경향우발생재고BMI병례중.결론 MMR기인돌변여자궁내막복잡불전형증생급자궁내막암적발생유일정적상관성,단재림상병리지표상,제MSH6결실표체여고BMI상관외,기타4항MMR지표여자궁내막암적종류분기화조직학분급무명현상관성.
Objective To investigate the expression of DNA mismatch repair (MMR) genes (MLH1,MSH2,MSH6 and PMS2) in endometrial adenocarcinoma (EC) of patients under 50 years and to explore the relationship between MMR expression and clinicopathological features including body mass index (BMI),histological grade and pathological stage of EC.Methods MMR gene expression was investigated by immunohistochemical S-P method in endometrial adenocarcinomas of patients under age of 50.The control groups included complexity atypical hyperplasia endometrium (CAHE),simple hyperplasia endometrium (SHE),normal endometrium (NE) of patients under age of 50 and EC of patients older than 65 years.Results Twenty seven of 40 EC (67.5％) lost at least one MMR protein expression.Loss of at least one MMR protein expression was seen in 5/15 cases of CAHE,1/13 SHE and 1/11 NE,respectively (P＜0.01).The rates of loss of expression of MLH1,MSH2,MSH and PMS2 proteins in EC were 52.5％,12.5％,35.0％,and 30.0％,respectively.The difference between MLH1 and MSH6 expression among the four groups were significant (P＜0.05),but the expression of MSH2 showed no significant difference among the groups (P =0.295).The expression of MMR protein had no relationship with histological grade and pathological stage,although loss of MSH6 was more frequently seen in patients of higher BMI.Conclusions Abnormal expression of MMR proteins is correlated with the development of EC from complex atypical hyperplasia.With the exception of the correlation of MSH6 expression with higher BMI,the expression of MMR proteins in EC has no significant relationship with histological grade and pathological stage.
