CAJ | 학술논문

目的探讨RNA干扰Ezrin对胰腺癌胞系Panc-1细胞生物学行为的影响.方法建立靶向Ezrin干扰质粒,稳定转染Panc-1细胞,并用流式细胞术检测细胞周期,CCK-8法检测细胞增殖情况,扫描电镜观察细胞表面形态及表面突起的变化,软琼脂克隆形成实验检测细胞体外非锚着依赖性的生长能力,用未包被及包被Matrigel胶的Transwell小室分别检测细胞的运动和侵袭能力.结果 RNA干扰Ezrin后其蛋白的表达明显下降,Panc-1表面的细胞突起、微绒毛数量明显减少,体外非锚着依赖性的生长能力也明显下降,运动及侵袭能力也明显下降,但对细胞的体外增殖和细胞周期没有明显的影响.结论 Ezrin蛋白的表达对胰腺癌细胞的细胞突起、表面微绒毛的形成、细胞骨架及非锚着依赖性的生长发挥着重要的作用,干扰Ezrin的表达将对胰腺癌细胞的表面突起的形成、细胞骨架以至细胞运动及在转移灶的存活中起着重要的作用.
목적 탐토RNA간우Ezrin대이선암포계Panc-1세포생물학행위적영향.방법 건립파향Ezrin간우질립,은정전염Panc-1세포,병용류식세포술검측세포주기,CCK-8법검측세포증식정황,소묘전경관찰세포표면형태급표면돌기적변화,연경지극륭형성실험검측세포체외비묘착의뢰성적생장능력,용미포피급포피Matrigel효적Transwell소실분별검측세포적운동화침습능력.결과 RNA간우Ezrin후기단백적표체명현하강,Panc-1표면적세포돌기、미융모수량명현감소,체외비묘착의뢰성적생장능력야명현하강,운동급침습능력야명현하강,단대세포적체외증식화세포주기몰유명현적영향.결론 Ezrin단백적표체대이선암세포적세포돌기、표면미융모적형성、세포골가급비묘착의뢰성적생장발휘착중요적작용,간우Ezrin적표체장대이선암세포적표면돌기적형성、세포골가이지세포운동급재전이조적존활중기착중요적작용.
Objective To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.Methods Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid.The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis,respectively.Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy.Colony formation assay was used to determine the cell anchor-independent growth ability in vitro.Trans-filter migration and invasion assays were performed with 8 μm pore inserts in a 24-well BioCoat chamber with/without Matrigel.Results Ezrin silencing decreased cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion,but had no effects on cell proliferation in vitro and cell cycle,in pancreatic cancer cell line Panc-1.Conclusion Ezrin expression affects the cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion in pancreatic cancer cell line Panc-1.