CAJ | 학술논문

目的探讨空泡膜蛋白1(Vmp1)在乳腺浸润性导管癌(IDC)和含导管原位癌(DCIS)成分的IDC中的表达及其与预后的关系.方法采用免疫组织化学SP法检测Vmp1在102例IDC(其中32例为含DCIS成分的IDC)中的表达和定位,并与其他多项临床病理参数进行比较分析.结果 Vmp1阳性着色部位为细胞质,阳性率为57.8％ (59/102),IDC中Vmp1的表达与不同组织学分级(x2=12.644,P =0.002)、pTNM分期(x2=11.987,P=0.001)、淋巴结状态(x2=9.341,P=0.002)、肿物最大径(x2=7.630,P=0.022)、诺丁汉预后指数(NPI;x2=15.561,P=0.000)密切相关.在32例含有DCIS成分的IDC病例中,Vmp1表达的阳性率在DCIS中为81.3％ (26/32)比其旁边的IDC中56.3％ (18/32)要高,且差异有统计学意义(x2=4.655,P=0.031).患者术后平均无瘤生存期81.2个月,5年总生存率为90.2％ (92/102),无瘤生存率为81.4％ (83/102).Vmp1的表达对无瘤生存曲线分布有明显影响,其中阴性者预后较差(x2=11.192,P=0.001),多因素分析显示Vmp1是一个保护因素,相对危险度＜1.结论 IDC组织中Vmp1的表达具有较好的估测预后价值,Vmp1阳性提示预后较好.
목적 탐토공포막단백1(Vmp1)재유선침윤성도관암(IDC)화함도관원위암(DCIS)성분적IDC중적표체급기여예후적관계.방법 채용면역조직화학SP법검측Vmp1재102례IDC(기중32례위함DCIS성분적IDC)중적표체화정위,병여기타다항림상병리삼수진행비교분석.결과 Vmp1양성착색부위위세포질,양성솔위57.8％ (59/102),IDC중Vmp1적표체여불동조직학분급(x2=12.644,P =0.002)、pTNM분기(x2=11.987,P=0.001)、림파결상태(x2=9.341,P=0.002)、종물최대경(x2=7.630,P=0.022)、낙정한예후지수(NPI;x2=15.561,P=0.000)밀절상관.재32례함유DCIS성분적IDC병례중,Vmp1표체적양성솔재DCIS중위81.3％ (26/32)비기방변적IDC중56.3％ (18/32)요고,차차이유통계학의의(x2=4.655,P=0.031).환자술후평균무류생존기81.2개월,5년총생존솔위90.2％ (92/102),무류생존솔위81.4％ (83/102).Vmp1적표체대무류생존곡선분포유명현영향,기중음성자예후교차(x2=11.192,P=0.001),다인소분석현시Vmp1시일개보호인소,상대위험도＜1.결론 IDC조직중Vmp1적표체구유교호적고측예후개치,Vmp1양성제시예후교호.
Objective To investigate the expression of vacuole membrane protein 1 (Vmpl) and its prognostic value in invasive ductal carcinoma (IDC) and concomitant ductal carcinoma in situ (DCIS) of the breast.Methods The expression and location of Vmp1 in the breast tissues from 102 patients with IDC and 32 concomitant DCIS were detected by immunohistochemical SP method,and the relationship with clinicopathologic parameters was analyzed.Results Vmp1 expression was observed in the cytoplasm of the cancer cells in 57.8％ (59/102) cases,and correlated with grade (x2 =12.644,P =0.002),pTNM stage (x2 =11.987,P =0.001),node status (x2 =9.341,P =0.002),tumor diameter (x2 =7.630,P =0.022) as well as Nottingham Prognostic Index (NPI;x2 =15.561,P =0.000).The expression of Vmp1 in concomitant DCIS was higher than that in IDC (81.3％ vs 56.3％ ; x2 =4.655,P =0.031).In this cohort,the mean disease-free survival was 81.2 months; the 5-year overall survival rate was 90.2％ (92/102) and the disease-free survival rate was 81.4％ (83/102).Vmp1 expression had significant influence on disease-free survival time,with Vmp1-negative patients showing poor prognosis (x2 =11.192,P =0.001).COX's proportional hazards regression model revealed that Vmp1 was a protective factor,with relative risk ＜ 1.Conclusions The detection of Vmp1 in IDC may be helpful for prognosis prediction; the patients with Vmp1 expression may have a better prognosis.