中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2013年
2期
111-115
,共5页
刘勇%李果%粟忠武%邱元正%张欣%余长云%周小娟%任舒灵%黄东海%田勇泉
劉勇%李果%粟忠武%邱元正%張訢%餘長雲%週小娟%任舒靈%黃東海%田勇泉
류용%리과%속충무%구원정%장흔%여장운%주소연%임서령%황동해%전용천
喉肿瘤%癌,鳞状细胞%肿瘤转移
喉腫瘤%癌,鱗狀細胞%腫瘤轉移
후종류%암,린상세포%종류전이
Laryngeal neoplasms%Carcinoma,squamous cell%Neoplasm metastasis
目的 检测星形胶质细胞上调基因1(AEG-1)在喉鳞状细胞癌(鳞癌)组织中的表达,并探讨其表达与喉鳞癌临床病理特征和预后之间的关系.方法 逆转录聚合酶链反应(RT-PCR)测定AEG-1 mRNA在13对配对喉鳞癌及癌旁黏膜组织中的表达.同时,免疫组织化学技术检测AEG-1蛋白在88例喉鳞癌及15例癌旁黏膜组织中的表达,并统计分析其表达水平与临床病理特征及预后的关系.结果 AEG-I蛋白在15例癌旁黏膜组织中仅呈阴性或弱阳性表达；AEG-1 mRNA在喉鳞癌组织中的相对表达量较癌旁黏膜显著升高(0.81 ±0.17比0.23 ±0.10;t=10.337,P＜0.01)；88例喉鳞癌组织中,AEG-1蛋白的阳性表达率为87.5％(77/88).且AEG-1蛋白表达水平与喉鳞癌患者T分期(x2 =6.289,P =0.018)、临床分期(x2=11.049,P＜0.01)、转移(x2=20.859,P＜0.01)及术后复发(x2=13.459,P＜0.01)密切相关.生存分析显示AEG-1蛋白高表达组与低表达组患者5年总生存率分别为35.9％与86.4％,差异具有统计学意义(x2=23.409,P＜0.01)；多因素分析示AEG-1蛋白表达水平为喉鳞癌患者预后的独立影响因素(P=0.016).结论 AEG-1蛋白可能在喉鳞癌的发生、发展中发挥了重要作用,对喉鳞癌患者的预后具有评估价值.
目的 檢測星形膠質細胞上調基因1(AEG-1)在喉鱗狀細胞癌(鱗癌)組織中的錶達,併探討其錶達與喉鱗癌臨床病理特徵和預後之間的關繫.方法 逆轉錄聚閤酶鏈反應(RT-PCR)測定AEG-1 mRNA在13對配對喉鱗癌及癌徬黏膜組織中的錶達.同時,免疫組織化學技術檢測AEG-1蛋白在88例喉鱗癌及15例癌徬黏膜組織中的錶達,併統計分析其錶達水平與臨床病理特徵及預後的關繫.結果 AEG-I蛋白在15例癌徬黏膜組織中僅呈陰性或弱暘性錶達；AEG-1 mRNA在喉鱗癌組織中的相對錶達量較癌徬黏膜顯著升高(0.81 ±0.17比0.23 ±0.10;t=10.337,P＜0.01)；88例喉鱗癌組織中,AEG-1蛋白的暘性錶達率為87.5％(77/88).且AEG-1蛋白錶達水平與喉鱗癌患者T分期(x2 =6.289,P =0.018)、臨床分期(x2=11.049,P＜0.01)、轉移(x2=20.859,P＜0.01)及術後複髮(x2=13.459,P＜0.01)密切相關.生存分析顯示AEG-1蛋白高錶達組與低錶達組患者5年總生存率分彆為35.9％與86.4％,差異具有統計學意義(x2=23.409,P＜0.01)；多因素分析示AEG-1蛋白錶達水平為喉鱗癌患者預後的獨立影響因素(P=0.016).結論 AEG-1蛋白可能在喉鱗癌的髮生、髮展中髮揮瞭重要作用,對喉鱗癌患者的預後具有評估價值.
목적 검측성형효질세포상조기인1(AEG-1)재후린상세포암(린암)조직중적표체,병탐토기표체여후린암림상병리특정화예후지간적관계.방법 역전록취합매련반응(RT-PCR)측정AEG-1 mRNA재13대배대후린암급암방점막조직중적표체.동시,면역조직화학기술검측AEG-1단백재88례후린암급15례암방점막조직중적표체,병통계분석기표체수평여림상병리특정급예후적관계.결과 AEG-I단백재15례암방점막조직중부정음성혹약양성표체；AEG-1 mRNA재후린암조직중적상대표체량교암방점막현저승고(0.81 ±0.17비0.23 ±0.10;t=10.337,P＜0.01)；88례후린암조직중,AEG-1단백적양성표체솔위87.5％(77/88).차AEG-1단백표체수평여후린암환자T분기(x2 =6.289,P =0.018)、림상분기(x2=11.049,P＜0.01)、전이(x2=20.859,P＜0.01)급술후복발(x2=13.459,P＜0.01)밀절상관.생존분석현시AEG-1단백고표체조여저표체조환자5년총생존솔분별위35.9％여86.4％,차이구유통계학의의(x2=23.409,P＜0.01)；다인소분석시AEG-1단백표체수평위후린암환자예후적독립영향인소(P=0.016).결론 AEG-1단백가능재후린암적발생、발전중발휘료중요작용,대후린암환자적예후구유평고개치.
Objective To assess the protein expression ot astrocyte elevated gene-1 (AEG-1) in tissue specimens of laryngeal squamous cell carcinoma (LSCC),and to correlate its expression with clinicopathological paralneters and prognosis in patients with LSCC.Methods RT-PCR was used to assay the expression of AEG-1 mRNA in 13 pairs of LSCC tissues and their corresponding noncarcinoma epithelia.Immunohistochemistry was performed on paraffin-embedded tissue specimens to investigate the protein expression of AEG-1 in 88 cases of LSCC specimens and 15 cases of adjacent epithelial samples.Results The expression of AEG-1 mRNA was significantly increased in LSCC tissues compared to adjacent noncarcinoma epithelial tissues (0.81 ± 0.17 vs.0.23 ± 0.10; t =10.337,P ＜ 0.001).Meantime,the positive rate of AEG-1 protein in 88 cases of LSCC was 87.5％ (77/88).However,15 cases of adjacent noncarcinoma epithelial merely demonstrated negative or mild expression of AEG-1 protein.AEG-1 overexpression was closely correlated with T stage (x2 =6.289,P =0.018),clinical stage (x2 =11.049,P ＜ 0.01),metastasis (x2 =20.859,P ＜ 0.01) and recurrence (x2 =13.459,P ＜ 0.01).The overall survival rates of patients with AEG-I overexpression and low expression were 35.9％ and 86.4％,respectively (x2 =23.409,P ＜0.01).Multivariate Cox regression analysis revealed that AEG-1 expression was an independent prognostic factor(P =0.016).Conclusion AEG-1 protein may play a critical role in the initiation and progression of LSCC,implicating its predictive value in prognosis.
