CAJ | 학술논문

目的探讨碳酸酐酶Ⅸ蛋白在乳腺癌分子分型中的表达及其意义.方法采用免疫组织化学MaxVision法检测117例乳腺浸润性导管癌中雌激素受体(ER)、孕激素受体(PR)、HER2、CK5/6、表皮生长因子受体(EGFR)、碳酸酐酶Ⅸ的表达.结果浸润性导管癌患者年龄25 ～ 71岁,平均49.6岁.117例中分5型:管腔A型66例(56.4％)、管腔B型6例(5.1％)、HER2过表达型10例(8.6％)、基底细胞样型20例(17.1％)、未分类型15例(12.8％).碳酸酐酶Ⅸ在管腔A型和基底细胞样型乳腺癌中的表达比例分别为13.6％ (9/66)和8/20,差异有统计学意义(P＜0.05).管腔A型中碳酸酐酶Ⅸ的阳性表达率,肿瘤最大径＞2 cm组(7/27,25.9％)明显高于≤2 cm组(2/39,5.1％),差异有统计学意义(P＜0.05).碳酸酐酶Ⅸ在组织学3级(18/50,36.0％)中的表达明显高于1级(2/21,9.5％)和2级(7/46,15.2％),差异有统计学意义(均P=0.006).碳酸酐酶Ⅸ表达阴性的病例中,ER和PR的阳性率分别为61.1％ (55/90)和55.6％ (50/90)；碳酸酐酶Ⅸ表达阳性的病例中,ER和PR的阳性率分别为37.0％(10/27)和29.6％(8/27).碳酸酐酶Ⅸ表达阴性组激素受体的阳性率均明显高于阳性表达组,二者差异有统计学意义(均P＜0.05).结论碳酸酐酶Ⅸ表达不仅与乳腺浸润性导管癌的分子分型有关,还与组织学分级、激素受体表达情况、肿瘤大小有关.碳酸酐酶Ⅸ是乳腺癌预后差的相对独立的标志物.
목적 탐토탄산항매Ⅸ단백재유선암분자분형중적표체급기의의.방법 채용면역조직화학MaxVision법검측117례유선침윤성도관암중자격소수체(ER)、잉격소수체(PR)、HER2、CK5/6、표피생장인자수체(EGFR)、탄산항매Ⅸ적표체.결과 침윤성도관암환자년령25 ～ 71세,평균49.6세.117례중분5형:관강A형66례(56.4％)、관강B형6례(5.1％)、HER2과표체형10례(8.6％)、기저세포양형20례(17.1％)、미분류형15례(12.8％).탄산항매Ⅸ재관강A형화기저세포양형유선암중적표체비례분별위13.6％ (9/66)화8/20,차이유통계학의의(P＜0.05).관강A형중탄산항매Ⅸ적양성표체솔,종류최대경＞2 cm조(7/27,25.9％)명현고우≤2 cm조(2/39,5.1％),차이유통계학의의(P＜0.05).탄산항매Ⅸ재조직학3급(18/50,36.0％)중적표체명현고우1급(2/21,9.5％)화2급(7/46,15.2％),차이유통계학의의(균P=0.006).탄산항매Ⅸ표체음성적병례중,ER화PR적양성솔분별위61.1％ (55/90)화55.6％ (50/90)；탄산항매Ⅸ표체양성적병례중,ER화PR적양성솔분별위37.0％(10/27)화29.6％(8/27).탄산항매Ⅸ표체음성조격소수체적양성솔균명현고우양성표체조,이자차이유통계학의의(균P＜0.05).결론 탄산항매Ⅸ표체불부여유선침윤성도관암적분자분형유관,환여조직학분급、격소수체표체정황、종류대소유관.탄산항매Ⅸ시유선암예후차적상대독립적표지물.
Objective To study the expression of carbonic anhydrase (CA) Ⅸ and its significance in molecular subtyping of breast carcinomas.Method MaxVision immunohistochemical staining was used to examine the expression of ER,PR,HER2,CK5/6,EGFR,and CA Ⅸ in 117 cases of breast invasive ductal carcinomas.Results The patients' age ranged from 25 to 71 years (mean 49.6 years).All the 117 cases were subclassified into five subtypes,with 66 (56.4％) luminal A,6 (5.1％) luminal B,10(8.6％) HER2 positive,20(17.1％) basal-like,and 15(12.8％) unclassified tumors.The expression of CA Ⅸ in luminal A and basal-like breast cancers was 13.6％ (9/66) and 8/20,respectively,with a significant difference (P ＜ 0.05).Among the luminal A cancers,the expression of CA Ⅸ in tumors ＞ 2 cm (7/27,25.9％) was significantly (P ＜ 0.05) higher than that of tumors ≤2 cm (2/39,5.1％).The expression of CA Ⅸ in grade 3 invasive ductal carcinoma (18/50,36.0％) was significantly higher than that in grade 1 (2/21,9.5％) and 2 (7/46,15.2％) tumors (both P =0.006).In CA Ⅸ-negative of invasive ductal carcinoma,the expression of ER and PR was 61.1％ (55/90) and 55.6％ (50/90),respectively; whereas in CA Ⅸ-positive cancers,the expression of ER and PR was 37.0％ (10/27) and 29.6％ (8/27),respectively.The expression of hormone receptors in CA Ⅸ-negative tumors was significantly higher than that in CA Ⅸ-positive tumors (for both ER and PR,P ＜ 0.05).Conclusions The expression of CA Ⅸ correlates not only with molecular subtypes of breast cancer,but also with the grading,hormone receptors and diameter of mammary invasive ductal carcinoma.CA Ⅸ is a relative independent marker of poor prognosis in breast cancer.