中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2014年
2期
88-94
,共7页
王颖%阿迪拉·斯依提%张晓明%董颖%李文婷%周梅%李挺
王穎%阿迪拉·斯依提%張曉明%董穎%李文婷%週梅%李挺
왕영%아적랍·사의제%장효명%동영%리문정%주매%리정
子宫内膜肿瘤%微RNAs%PTEN磷酸水解酶
子宮內膜腫瘤%微RNAs%PTEN燐痠水解酶
자궁내막종류%미RNAs%PTEN린산수해매
Endometrial neoplasms%MicroRNAs%PTEN phosphohydrolase
目的 探讨典型子宫内膜样癌组织微小RNA(miRNA)的表达特点及与子宫内膜癌相关分子改变的内在关联性及其临床意义.方法 以TaqMan低密度芯片分析2例子宫内膜样癌组织miRNA的表达特点.筛选表达显著差异的miRNA,采用即时荧光定量聚合酶链反应技术检测73例子宫内膜癌组织中这些miRNA的表达.免疫组织化学法检测miRNA的预测靶基因PTEN表达.结果 (1) miRNA表达谱分析显示相对于增殖期内膜,典型Ⅰ型癌组织共有47个miRNA存在差异表达,其中26个表达降低,21个表达升高.(2)进一步筛选8种miRNA验证其表达:8种miRNA在58例Ⅰ型癌中的表达与表达谱结果一致,其中表达升高的3种miRNA(miR-141、miR-200a、miR-205)及表达降低的两种miRNA(miR-143和miR-145),差异均有统计学意义(P＜0.05),但此5种miRNA在Ⅱ型内膜癌中变化不显著.(3)Ⅰ型内膜癌中miR-141、miR-200a高表达组的PTEN失表达率更高,且miR-200a与PTEN表达呈负相关(P＜0.05).结论 Ⅰ型子宫内膜癌有相对特异的miRNA表达谱；miR-200a、miR-205、miR-141、miR-143、miR-145在Ⅰ型癌中变化显著,但在Ⅱ型癌中变化不显著,提示Ⅰ/Ⅱ型癌发病中发挥作用的miRNA不尽相同.Ⅰ型癌中miR-141和miR-200a可能通过对抑癌基因PTEN的靶向调控,参与子宫内膜癌的形成及演进.
目的 探討典型子宮內膜樣癌組織微小RNA(miRNA)的錶達特點及與子宮內膜癌相關分子改變的內在關聯性及其臨床意義.方法 以TaqMan低密度芯片分析2例子宮內膜樣癌組織miRNA的錶達特點.篩選錶達顯著差異的miRNA,採用即時熒光定量聚閤酶鏈反應技術檢測73例子宮內膜癌組織中這些miRNA的錶達.免疫組織化學法檢測miRNA的預測靶基因PTEN錶達.結果 (1) miRNA錶達譜分析顯示相對于增殖期內膜,典型Ⅰ型癌組織共有47箇miRNA存在差異錶達,其中26箇錶達降低,21箇錶達升高.(2)進一步篩選8種miRNA驗證其錶達:8種miRNA在58例Ⅰ型癌中的錶達與錶達譜結果一緻,其中錶達升高的3種miRNA(miR-141、miR-200a、miR-205)及錶達降低的兩種miRNA(miR-143和miR-145),差異均有統計學意義(P＜0.05),但此5種miRNA在Ⅱ型內膜癌中變化不顯著.(3)Ⅰ型內膜癌中miR-141、miR-200a高錶達組的PTEN失錶達率更高,且miR-200a與PTEN錶達呈負相關(P＜0.05).結論 Ⅰ型子宮內膜癌有相對特異的miRNA錶達譜；miR-200a、miR-205、miR-141、miR-143、miR-145在Ⅰ型癌中變化顯著,但在Ⅱ型癌中變化不顯著,提示Ⅰ/Ⅱ型癌髮病中髮揮作用的miRNA不儘相同.Ⅰ型癌中miR-141和miR-200a可能通過對抑癌基因PTEN的靶嚮調控,參與子宮內膜癌的形成及縯進.
목적 탐토전형자궁내막양암조직미소RNA(miRNA)적표체특점급여자궁내막암상관분자개변적내재관련성급기림상의의.방법 이TaqMan저밀도심편분석2례자궁내막양암조직miRNA적표체특점.사선표체현저차이적miRNA,채용즉시형광정량취합매련반응기술검측73례자궁내막암조직중저사miRNA적표체.면역조직화학법검측miRNA적예측파기인PTEN표체.결과 (1) miRNA표체보분석현시상대우증식기내막,전형Ⅰ형암조직공유47개miRNA존재차이표체,기중26개표체강저,21개표체승고.(2)진일보사선8충miRNA험증기표체:8충miRNA재58례Ⅰ형암중적표체여표체보결과일치,기중표체승고적3충miRNA(miR-141、miR-200a、miR-205)급표체강저적량충miRNA(miR-143화miR-145),차이균유통계학의의(P＜0.05),단차5충miRNA재Ⅱ형내막암중변화불현저.(3)Ⅰ형내막암중miR-141、miR-200a고표체조적PTEN실표체솔경고,차miR-200a여PTEN표체정부상관(P＜0.05).결론 Ⅰ형자궁내막암유상대특이적miRNA표체보；miR-200a、miR-205、miR-141、miR-143、miR-145재Ⅰ형암중변화현저,단재Ⅱ형암중변화불현저,제시Ⅰ/Ⅱ형암발병중발휘작용적miRNA불진상동.Ⅰ형암중miR-141화miR-200a가능통과대억암기인PTEN적파향조공,삼여자궁내막암적형성급연진.
Objective To determine the microRNA (miRNA) expression signature and its clinical significance in endometrioid carcinoma (EC).Methods The miRNA profiles were analyzed by miRNA microarray in 73 cases of EC.The expression level of the eight selected miRNAs were measured by real-time fluorescent quatitative PCR(qRT-PCR).Immunohistochemistry (IHC) was performed to assess the status of PTEN,a potential target of the selected miRNAs.Results (1) Using TaqMan low-density arrays,47 miRNAs that differed between EC and normal controls were identified,including 26 down-regulated and 21 up-regulated miRNAs.(2) To confirm the miRNA expression pattern in type Ⅰ EC,the expression levels of the eight selected miRNAs were evaluated in a new set of 58 cases of type Ⅰ EC by individual miRNA qRT-PCR assays.Three miRNAs (miR-141,miR-200a,miR-205) were up-regulated and two miRNAs (miR-143,miR-145) were down-regulated.These were significantly differentially expressed in type Ⅰ EC and normal controls (P ＜ 0.05),whereas such difference was not present in type Ⅱ tumors compared to normal controls.(3) In type Ⅰ EC,loss of PTEN was more frequent in the miR-141 or miR-200a up-regulated subgroups,and the correlation between the PTEN and miR-200a status in type Ⅰ tumors was statistically significant (P ＜0.05).Conclusions EC may have a unique miRNA expression profile.The expression levels of the five miRNAs (miR-141,miR-200a,miR-205,miR-143,miR-145) are significantly deregulated in type Ⅰ EC compared to normal control but not in type Ⅱ tumors.The findings suggest that the miRNAs related to type Ⅰ and type Ⅱ EC might be different.PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis.
