中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2014年
2期
109-113
,共5页
于品%鲍琳琳%许黎黎%李枫棣%吕琦%姚艳丰%秦川
于品%鮑琳琳%許黎黎%李楓棣%呂琦%姚豔豐%秦川
우품%포림림%허려려%리풍체%려기%요염봉%진천
小鼠%模型,动物%显微镜检查,电子,透射%肌,骨骼%中枢神经系统
小鼠%模型,動物%顯微鏡檢查,電子,透射%肌,骨骼%中樞神經繫統
소서%모형,동물%현미경검사,전자,투사%기,골격%중추신경계통
Mice%Models,animal%Microscopy,electron,transmission%Muscle,skeletal%Central nervous system
目的 观察感染肠道病毒71型(EV71)小鼠模型的脑、脊髓、肌肉、空肠和肺脏组织超微结构改变,探讨EV71嗜肌性发病机制和神经系统病理改变的发展过程.方法 采用腹腔注射EV71感染10日龄BALB/c乳鼠,感染4d出现症状后,取其脑、脊髓、肌肉、空肠以及肺脏组织进行透射电镜和光镜观察.结果 感染组小鼠脑组织病变轻微,部分线粒体内室肿胀,有髓神经纤维髓鞘呈现小泡性分离；脊髓前角神经元尼氏小体溶解,粗面内质网脱颗粒,线粒体肿胀,胞质呈筛状外观,并可见嗜神经现象；肌组织发生退行性变,横纹消失,肌细胞核固缩,线粒体肿胀；空肠黏膜上皮细胞微绒毛排列疏松,形成螺旋状髓鞘样结构,线粒体肿胀；肺组织发生了间质性肺炎,肺泡Ⅱ型上皮细胞增生,其嗜锇性板层小体或出现排空现象.结论 感染EV71小鼠模型其骨骼肌发生严重的坏死性肌炎,病毒表现出强肌嗜性；中枢神经系统发生不同程度的损伤,脊髓前角运动神经元损伤,这与肢体瘫痪有一定的关联性.
目的 觀察感染腸道病毒71型(EV71)小鼠模型的腦、脊髓、肌肉、空腸和肺髒組織超微結構改變,探討EV71嗜肌性髮病機製和神經繫統病理改變的髮展過程.方法 採用腹腔註射EV71感染10日齡BALB/c乳鼠,感染4d齣現癥狀後,取其腦、脊髓、肌肉、空腸以及肺髒組織進行透射電鏡和光鏡觀察.結果 感染組小鼠腦組織病變輕微,部分線粒體內室腫脹,有髓神經纖維髓鞘呈現小泡性分離；脊髓前角神經元尼氏小體溶解,粗麵內質網脫顆粒,線粒體腫脹,胞質呈篩狀外觀,併可見嗜神經現象；肌組織髮生退行性變,橫紋消失,肌細胞覈固縮,線粒體腫脹；空腸黏膜上皮細胞微絨毛排列疏鬆,形成螺鏇狀髓鞘樣結構,線粒體腫脹；肺組織髮生瞭間質性肺炎,肺泡Ⅱ型上皮細胞增生,其嗜鋨性闆層小體或齣現排空現象.結論 感染EV71小鼠模型其骨骼肌髮生嚴重的壞死性肌炎,病毒錶現齣彊肌嗜性；中樞神經繫統髮生不同程度的損傷,脊髓前角運動神經元損傷,這與肢體癱瘓有一定的關聯性.
목적 관찰감염장도병독71형(EV71)소서모형적뇌、척수、기육、공장화폐장조직초미결구개변,탐토EV71기기성발병궤제화신경계통병리개변적발전과정.방법 채용복강주사EV71감염10일령BALB/c유서,감염4d출현증상후,취기뇌、척수、기육、공장이급폐장조직진행투사전경화광경관찰.결과 감염조소서뇌조직병변경미,부분선립체내실종창,유수신경섬유수초정현소포성분리；척수전각신경원니씨소체용해,조면내질망탈과립,선립체종창,포질정사상외관,병가견기신경현상；기조직발생퇴행성변,횡문소실,기세포핵고축,선립체종창；공장점막상피세포미융모배렬소송,형성라선상수초양결구,선립체종창；폐조직발생료간질성폐염,폐포Ⅱ형상피세포증생,기기철성판층소체혹출현배공현상.결론 감염EV71소서모형기골격기발생엄중적배사성기염,병독표현출강기기성；중추신경계통발생불동정도적손상,척수전각운동신경원손상,저여지체탄탄유일정적관련성.
Objective To document ultrastructural changes of brain,spinal cord,skeletal muscle,jejunum and lung of EV71 infection mouse model,and to explore the myotropism and pathogenesis of EV71 in nervous system.Methods Ten-day-old suckling mice were infected with EV71 strain via the intraperitoneal route.Mice with paralysis were scarified on day 4 post infection and the brain,spinal cord,skeletal muscle,jejunum and lung were sampled for transmission electron microscopy and light microscopy.Results Lesions in brain were generally mild with inner chamber swelling in some of mitochondria.Myelin sheaths of medullated fibers were split with vacuolated changes.The Nissl bodies in anterior motor neurons disappeared along with mitochondria swelling,rough endoplasmic reticulum swelling and degranulation.Cytoplasm of anterior motor neurons showed cribriform appearance accompanied by neuronophagia.The bands of skeletal muscle in the infected group disappeared with degeneration and karyopyknosis in myocytes,in addition to mitochondrial swelling.Microvilli of epithelium in jejunum became loosely arranged along with formation of spiral medullary sheath structure and mitochondria swelling.Interstitial pneumonia was observed in lungs with type Ⅱ pneumocyte proliferation and evacuation of the multilamellar bodies.Conclusions EV71 infection causes severe myositis in the mouse model suggesting a strong myotropism of EV71 virus.The presence of lesions of various degrees in central nervous system and changes in anterior motor neurons may be associated with limb paralysis.
