中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2014年
7期
447-450
,共4页
王晓江%郑雄伟%林贤东%师怡%何银珠%陈刚
王曉江%鄭雄偉%林賢東%師怡%何銀珠%陳剛
왕효강%정웅위%림현동%사이%하은주%진강
乳腺肿瘤%乳腺疾病%甲基化%免疫组织化学
乳腺腫瘤%乳腺疾病%甲基化%免疫組織化學
유선종류%유선질병%갑기화%면역조직화학
Breast neoplasms%Breast diseases%Methylation%Immunohistochemistry
目的 探讨不同类型乳腺组织中人runt相关转录因子3(Runx3)的蛋白表达及启动子甲基化的差异.方法 收集2010年2月至2012年8月间乳腺病变标本114例,包括纤维腺瘤35例、导管内癌39例、浸润性导管癌40例,选取浸润性导管癌的癌旁正常乳腺组织33例作为对照.免疫组织化学SP法检测Runx3蛋白在各类乳腺组织中的表达.高分辨率熔解曲线(HRM)分析各型组织中Runx3启动子甲基化程度.结果 Runx3蛋白主要表达于乳腺导管上皮细胞的细胞质.在正常乳腺组织、纤维腺瘤、导管内癌和浸润性导管癌中,Runx3蛋白的阳性率分别为87.9％ (29/33)、85.7％(30/35)、53.8％ (21/39)、40.0％ (16/40);而Runx3启动子甲基化率则分别为12.1％ (4/33)、20.0％(7/35)、46.2％ (18/39)、57.5％ (23/40).不同乳腺组织中Runx3启动子甲基化及其蛋白表达的相关性:正常乳腺组织、纤维腺瘤、导管内癌、浸润性导管癌组织中的r值分别为-0.431(P =0.012)、-0.408(P =0.015)、-0.589(P =0.000)、-0.743(P =0.000).结论 乳腺导管内癌和浸润性导管癌中Runx3蛋白的表达呈下降趋势,其启动子甲基化明显增高;Runx3基因启动子甲基化可能是乳腺癌发生发展的重要因素之一.
目的 探討不同類型乳腺組織中人runt相關轉錄因子3(Runx3)的蛋白錶達及啟動子甲基化的差異.方法 收集2010年2月至2012年8月間乳腺病變標本114例,包括纖維腺瘤35例、導管內癌39例、浸潤性導管癌40例,選取浸潤性導管癌的癌徬正常乳腺組織33例作為對照.免疫組織化學SP法檢測Runx3蛋白在各類乳腺組織中的錶達.高分辨率鎔解麯線(HRM)分析各型組織中Runx3啟動子甲基化程度.結果 Runx3蛋白主要錶達于乳腺導管上皮細胞的細胞質.在正常乳腺組織、纖維腺瘤、導管內癌和浸潤性導管癌中,Runx3蛋白的暘性率分彆為87.9％ (29/33)、85.7％(30/35)、53.8％ (21/39)、40.0％ (16/40);而Runx3啟動子甲基化率則分彆為12.1％ (4/33)、20.0％(7/35)、46.2％ (18/39)、57.5％ (23/40).不同乳腺組織中Runx3啟動子甲基化及其蛋白錶達的相關性:正常乳腺組織、纖維腺瘤、導管內癌、浸潤性導管癌組織中的r值分彆為-0.431(P =0.012)、-0.408(P =0.015)、-0.589(P =0.000)、-0.743(P =0.000).結論 乳腺導管內癌和浸潤性導管癌中Runx3蛋白的錶達呈下降趨勢,其啟動子甲基化明顯增高;Runx3基因啟動子甲基化可能是乳腺癌髮生髮展的重要因素之一.
목적 탐토불동류형유선조직중인runt상관전록인자3(Runx3)적단백표체급계동자갑기화적차이.방법 수집2010년2월지2012년8월간유선병변표본114례,포괄섬유선류35례、도관내암39례、침윤성도관암40례,선취침윤성도관암적암방정상유선조직33례작위대조.면역조직화학SP법검측Runx3단백재각류유선조직중적표체.고분변솔용해곡선(HRM)분석각형조직중Runx3계동자갑기화정도.결과 Runx3단백주요표체우유선도관상피세포적세포질.재정상유선조직、섬유선류、도관내암화침윤성도관암중,Runx3단백적양성솔분별위87.9％ (29/33)、85.7％(30/35)、53.8％ (21/39)、40.0％ (16/40);이Runx3계동자갑기화솔칙분별위12.1％ (4/33)、20.0％(7/35)、46.2％ (18/39)、57.5％ (23/40).불동유선조직중Runx3계동자갑기화급기단백표체적상관성:정상유선조직、섬유선류、도관내암、침윤성도관암조직중적r치분별위-0.431(P =0.012)、-0.408(P =0.015)、-0.589(P =0.000)、-0.743(P =0.000).결론 유선도관내암화침윤성도관암중Runx3단백적표체정하강추세,기계동자갑기화명현증고;Runx3기인계동자갑기화가능시유선암발생발전적중요인소지일.
Objective To investigate the methylation status of Runx3 promoter and Runx3 expression in breast lesion tissues.Methods One hundred and fourteen breast lesions,including 35 cases of fibroadenoma,39 cases of intraductal carcinoma,40 cases of invasive ductal carcinoma,and 33 cases of normal breast tissue from Fabruary 2010 to August 2012 were included in this study.Runx3 protein expression was assessed by immunohistochemical SP method; whereas methylation of Runx3 promoter was assessed by high resolution melting (HRM) analysis.Results Runx3 protein was mainly expressed in the cytoplasm of ductal epithelial cells.The expression rates of Runx3 in normal breast tissue,fibroadenoma,ductal carcinoma in situ,invasive ductal carcinoma were 87.9％ (29/33),85.7％ (30/35),53.8％ (21/39),and 40.0％ (16/40) respectively.The methylation rates of Runx3 promoter were 12.1％ (4/33),20.0％ (7/35),46.2％ (18/39),and 57.5％ (23/40),respectively.Correlation analysis between promoter methylation and protein expression of Runx3 in different breast tissue showed the r value in normal breast tissue,fibroadenoma,ductal carcinoma in situ and invasive ductal carcinoma was-0.431 (P =0.012),-0.408 (P =0.015),-0.589 (P =0.000) and-0.743 (P =0.000) respectively.Conclusions Runx3 protein expression shows a downward trend in ductal carcinoma in situ and invasive ductal carcinoma,meanwhile its promoter methylation increases significantly.The methylation of Runx3 promoter may be one of the important factors in the occurrence and development of breast cancer.
