中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2014年
7期
473-477
,共5页
杨迷玲%王立峰%杨金花%杨广英
楊迷玲%王立峰%楊金花%楊廣英
양미령%왕립봉%양금화%양엄영
胃肿瘤%血管内皮生长因子类%微血管%新生血管化,病理性
胃腫瘤%血管內皮生長因子類%微血管%新生血管化,病理性
위종류%혈관내피생장인자류%미혈관%신생혈관화,병이성
Stomach neoplasms%Vascular endothelial growth factors%Microvessels%Neovascularization,pathologic
目的 探讨Versican在胃癌组织中的表达及其与胃癌血管生成的关系.方法 用免疫组织化学EliVision方法检测80例患者胃癌组织和30例患者癌旁正常组织中Versican和血管内皮生长因子(VEGF)的表达,CD34标记胃癌的微血管密度(MVD).结果 (1)胃癌组织中的Versican、VEGF和MVD均显著高于正常胃黏膜(P＜0.05).(2)在胃癌中Versican主要表达于间质纤维母细胞的胞质,且其表达与胃癌的分化程度、临床分期及淋巴结转移相关(P＜0.05),VEGF主要表达于癌细胞的胞质,且与胃癌的分化程度、临床分期、Lauren分型及淋巴结转移显著相关(P＜0.05).(3)胃癌组织中的MVD与分化程度、临床分期、Lauren分型、浸润深度及淋巴结转移显著相关(P＜0.05).(4)在胃癌组织中Versican与VEGF的表达呈正相关(r=0.467,P＜0.01).结论 Versican和VEGF的表达与胃癌的血管生成关系密切,二者协同作用促进胃癌的血管生成.
目的 探討Versican在胃癌組織中的錶達及其與胃癌血管生成的關繫.方法 用免疫組織化學EliVision方法檢測80例患者胃癌組織和30例患者癌徬正常組織中Versican和血管內皮生長因子(VEGF)的錶達,CD34標記胃癌的微血管密度(MVD).結果 (1)胃癌組織中的Versican、VEGF和MVD均顯著高于正常胃黏膜(P＜0.05).(2)在胃癌中Versican主要錶達于間質纖維母細胞的胞質,且其錶達與胃癌的分化程度、臨床分期及淋巴結轉移相關(P＜0.05),VEGF主要錶達于癌細胞的胞質,且與胃癌的分化程度、臨床分期、Lauren分型及淋巴結轉移顯著相關(P＜0.05).(3)胃癌組織中的MVD與分化程度、臨床分期、Lauren分型、浸潤深度及淋巴結轉移顯著相關(P＜0.05).(4)在胃癌組織中Versican與VEGF的錶達呈正相關(r=0.467,P＜0.01).結論 Versican和VEGF的錶達與胃癌的血管生成關繫密切,二者協同作用促進胃癌的血管生成.
목적 탐토Versican재위암조직중적표체급기여위암혈관생성적관계.방법 용면역조직화학EliVision방법검측80례환자위암조직화30례환자암방정상조직중Versican화혈관내피생장인자(VEGF)적표체,CD34표기위암적미혈관밀도(MVD).결과 (1)위암조직중적Versican、VEGF화MVD균현저고우정상위점막(P＜0.05).(2)재위암중Versican주요표체우간질섬유모세포적포질,차기표체여위암적분화정도、림상분기급림파결전이상관(P＜0.05),VEGF주요표체우암세포적포질,차여위암적분화정도、림상분기、Lauren분형급림파결전이현저상관(P＜0.05).(3)위암조직중적MVD여분화정도、림상분기、Lauren분형、침윤심도급림파결전이현저상관(P＜0.05).(4)재위암조직중Versican여VEGF적표체정정상관(r=0.467,P＜0.01).결론 Versican화VEGF적표체여위암적혈관생성관계밀절,이자협동작용촉진위암적혈관생성.
Objective To investigate the expression of Versican in gastric carcinoma and its relationship with tumor angiogenesis.Methods Protein expression of Versican,vascular endothelial growth factor and CD34 was evaluated by immunohistochemistry (EliVision method) in 80 cases of gastric carcinoma and 30 samples of normal gastric tissue.Results There were statistically significant differences in the expression of Versican,vascular endothelial growth factor and CD34 between gastric carcinoma and normal gastric tissue (P ＜ 0.05).The expression of Versican was seen mainly in fibroblasts of the tumor and was correlated with tumor differentiation,clinical stage and lymph node metastasis (P ＜ 0.05),whereas vascular endothelial growth factor was primarily seen in the cytoplasm of the tumor cells and correlated with tumor differentiation,clinical stage,Lauren classification and lymph node metastasis (P ＜0.05).MVD was correlated with tumor differentiation,clinical stage,Lauren classification,depth of tumor invasion and lymph node metastasis(P ＜0.05).In addition,positive correlation of Versican and VEGF protein expression was found in tumor cells (r =0.467,P ＜ 0,01).Conclusion The expression of both Versican and vascular endothelial growth factor is closely associated with tumor angiogenesis in gastric carcinoma.
