CAJ | 학술논문

目的观察神经干细胞(NSC)与原浆型星形胶质细胞(PAS)联合移植对大鼠损伤脊髓功能和形态的修复作用. 方法 60只Wistar大鼠,制成脊髓L1～L2左侧半切空洞损伤模型,采用随机数字表法分为损伤组(A组)、损伤后移植PAS组(B组)、移植NSC组(C组)、NSC和PAS按2:1比例联合移植组(D组),每组15只.伤后1,4,8周进行伤侧下肢BBB评分、体感诱发电位(SEP)和运动诱发电位(MEP)检测;8周损伤部位脊髓组织进行HE、Nissl、Holmes银染,观察移植物在损伤脊髓中的形态学变化. 结果 (1)行为学观察:4周时,各组间开始出现差异;8周时,组间差异更为明显,尤其D组大鼠最明显,已能行走,行走时频繁足底离地,并能与前后肢体协调运动,B、C组功能有一定改善,可支重性足底踏步,前后肢共济协调,但仍和D组存在明显差距,A组后肢关节活动幅度增加,足底仍不能着地支重.(2)电生理检查:1周时,各组MEP峰潜时(以N1波计算)和SEP峰潜时(以P1波计算)开始出现差异,至4,8周,B、C、D组峰潜时明显缩短,但D组缩短最为显著(P<0.01).(3)病理观察:8周时,A组缺损严重,新生组织较少,B、C、D组缺损组织基本填补完全,损伤空洞闭合,未见明显的炎症反应,B组损伤处可见大量形态不同、大小不等的新生胶质细胞,胶质纤维排列紊乱,病理染色未见神经元;C组损伤处新生组织较多,以神经胶质细胞为主,少量神经元分布,且可见再生纤维连接;D组损伤处新生组织填补缺损完全,胶质纤维中可见形态典型的新生神经元,数量明显多于C组. 结论 NSC和PAS联合移植到损伤脊髓组织后,能够存活、分化并从结构和功能上较好地修复组织缺损区域.
목적 관찰신경간세포(NSC)여원장형성형효질세포(PAS)연합이식대대서손상척수공능화형태적수복작용. 방법 60지Wistar대서,제성척수L1～L2좌측반절공동손상모형,채용수궤수자표법분위손상조(A조)、손상후이식PAS조(B조)、이식NSC조(C조)、NSC화PAS안2:1비례연합이식조(D조),매조15지.상후1,4,8주진행상측하지BBB평분、체감유발전위(SEP)화운동유발전위(MEP)검측;8주손상부위척수조직진행HE、Nissl、Holmes은염,관찰이식물재손상척수중적형태학변화. 결과 (1)행위학관찰:4주시,각조간개시출현차이;8주시,조간차이경위명현,우기D조대서최명현,이능행주,행주시빈번족저리지,병능여전후지체협조운동,B、C조공능유일정개선,가지중성족저답보,전후지공제협조,단잉화D조존재명현차거,A조후지관절활동폭도증가,족저잉불능착지지중.(2)전생리검사:1주시,각조MEP봉잠시(이N1파계산)화SEP봉잠시(이P1파계산)개시출현차이,지4,8주,B、C、D조봉잠시명현축단,단D조축단최위현저(P<0.01).(3)병리관찰:8주시,A조결손엄중,신생조직교소,B、C、D조결손조직기본전보완전,손상공동폐합,미견명현적염증반응,B조손상처가견대량형태불동、대소불등적신생효질세포,효질섬유배렬문란,병리염색미견신경원;C조손상처신생조직교다,이신경효질세포위주,소량신경원분포,차가견재생섬유련접;D조손상처신생조직전보결손완전,효질섬유중가견형태전형적신생신경원,수량명현다우C조. 결론 NSC화PAS연합이식도손상척수조직후,능구존활、분화병종결구화공능상교호지수복조직결손구역.
Objective To observe the morphological and functional recovery of rat spinal cord after transplantation of neural stem cell (NSC) and protoplasmic astrocyte (PAS). Methods Sixty Wistar rats, which were injured on the left side of L1-L2 spinal cord, were randomly divided into injury group ( Group A), PAS transplantation group ( Group B), NSC transplantation group ( Group C), combined PAS and NSC transplantation group ( Group D, NSC:PAS =2:1 ), 15 rats in each group. The BBB scale, motor evoked potential (MEP) and somatosensory evoked potential (SEP) of lower limb were examined at 1,4 and 8 weeks after injury. The injured spinal cords were stained by hematoxylin-eosin, Nissl and Holmes to observe the morphological changes of transplants at eight weeks after injury. Results (1) There was significant difference in behavior among groups at four weeks after injury. At eight weeks after injury, the hindlimb in Group D could support body weight, and forelimb could collaborate with hindlimb during movement. There were certain improvements of the behavior of rats in Groups B and C. The active extent of the hindlimb was improved, but the hindlimb still could not support body weight. There was significant difference among four groups. (2) At one week after injury, sensory and motor functions in all groups were rehabilitated differently. At four and eight weeks after injury, the latencies of peak N1 and P1 became shorter in Groups B, C and D, especially Group D (P<0.01 ). (3) At eight weeks after injury, the injury in Group A was much severe, with few cambiums. The cavity was basically closed in Groups B, C and D, with no obvious inflammation. Lots of diversified regenerative astrocytes were observed in Group B, and the gelatinous fibers were disorderly arrayed. No neuron was observed in Group B. Lots of cambiums (mainly astrocytes) and a few neurons could be seen in Group C, with the regenerated fibers connected. The cavity was fully closed in Group D, with more regenerative neurons with dendrites than in Group C. Conclusions NSC and PAS can not only survive and differentiate when they are transplanted to the injured spinal cord, but also repair the injured area in terms of structure and function.