中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2012年
9期
838-841
,共4页
方军%李华壮%丁刚%赵光宗%高克海%陈景春
方軍%李華壯%丁剛%趙光宗%高剋海%陳景春
방군%리화장%정강%조광종%고극해%진경춘
骨髓%间质干细胞%细胞分化%免疫抑制
骨髓%間質榦細胞%細胞分化%免疫抑製
골수%간질간세포%세포분화%면역억제
Bone marrow%Mesenchymal stem cells%Cell differentiation%Immunosuppression
目的 探讨骨向分化骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)的免疫学特性. 方法 正常成人骨髓分离的BMSCs在骨向诱导培养基中培养2周后,将未分化的BMSCs和骨向分化的BMSCs与同种异体T淋巴细胞、植物血凝素(PHA)共培养,MTT法检测T淋巴细胞的增殖情况；ELISA法测定骨向分化2周的BMSCs上清以及共同反应5d的混合淋巴细胞反应(mixed lymphocytes reaction,MLR)上清转化生长因子(transforming growth factor -β1,TGF -β1)的浓度,并通过加入抗TGF-β抗体检测MLR情况. 结果 无论是未分化的BMSCs还是骨向分化的BMSCs都不会引起同种异体T淋巴细胞的增殖,并且都能抑制PHA诱导的T淋巴细胞增殖.MLR中的TGF -β1浓度显著升高.抗TGF-β抗体能够抵消骨向分化的BMSCs免疫抑制作用. 结论 骨向分化的BMSCs具有低免疫原性和免疫抑制特性.
目的 探討骨嚮分化骨髓間充質榦細胞(bone mesenchymal stem cells,BMSCs)的免疫學特性. 方法 正常成人骨髓分離的BMSCs在骨嚮誘導培養基中培養2週後,將未分化的BMSCs和骨嚮分化的BMSCs與同種異體T淋巴細胞、植物血凝素(PHA)共培養,MTT法檢測T淋巴細胞的增殖情況；ELISA法測定骨嚮分化2週的BMSCs上清以及共同反應5d的混閤淋巴細胞反應(mixed lymphocytes reaction,MLR)上清轉化生長因子(transforming growth factor -β1,TGF -β1)的濃度,併通過加入抗TGF-β抗體檢測MLR情況. 結果 無論是未分化的BMSCs還是骨嚮分化的BMSCs都不會引起同種異體T淋巴細胞的增殖,併且都能抑製PHA誘導的T淋巴細胞增殖.MLR中的TGF -β1濃度顯著升高.抗TGF-β抗體能夠牴消骨嚮分化的BMSCs免疫抑製作用. 結論 骨嚮分化的BMSCs具有低免疫原性和免疫抑製特性.
목적 탐토골향분화골수간충질간세포(bone mesenchymal stem cells,BMSCs)적면역학특성. 방법 정상성인골수분리적BMSCs재골향유도배양기중배양2주후,장미분화적BMSCs화골향분화적BMSCs여동충이체T림파세포、식물혈응소(PHA)공배양,MTT법검측T림파세포적증식정황；ELISA법측정골향분화2주적BMSCs상청이급공동반응5d적혼합림파세포반응(mixed lymphocytes reaction,MLR)상청전화생장인자(transforming growth factor -β1,TGF -β1)적농도,병통과가입항TGF-β항체검측MLR정황. 결과 무론시미분화적BMSCs환시골향분화적BMSCs도불회인기동충이체T림파세포적증식,병차도능억제PHA유도적T림파세포증식.MLR중적TGF -β1농도현저승고.항TGF-β항체능구저소골향분화적BMSCs면역억제작용. 결론 골향분화적BMSCs구유저면역원성화면역억제특성.
Objective To investigate the immunologic properties of osteogenic differentiated bone mesenchymal stem cells (BMSCs). Methods BMSCs were isolated from normal volunteers and induced in osteogenic medium for two weeks. Then,non-differentiated/osteogenic differentiated BMSCs were co-cultured with allogenic T cells and phytohemagglutinin (PHA).The proliferation of T cells was examined by MTT method.The concentrations of TGF-β1 in osteogenic differentiated BMSCs supernatants at week 2 and mixed lymphocytes reaction (MLR) supernatants at day 5 were determined by ELISA.Also,anti-TGF-β antibody was added into the MLR to detect the response of the mixed T cells. Results Non-differentiated and osteogenic differentiated BMSCs did not induce proliferation of the allogeneic T cells but both suppressed the proliferation of the T cells mediated by PHA.The TGF-β1 concentrations had significant elevation in the MLR.Anti-TGF-β antibody could counteract the immunosuppressive function of the osteogenic differentiated BMSCs. Conclusion Osteogenic differentiated BMSCs possess low immunogenicity and immunosuppressive property.
