中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2012年
9期
849-853
,共5页
成肌细胞%移植%神经肌肉功能修复
成肌細胞%移植%神經肌肉功能脩複
성기세포%이식%신경기육공능수복
Myoblasts%Transplantation%Neuromuscular function repairation
目的 观察成肌细胞自体移植对大鼠神经植入术后终板再生及神经肌肉功能恢复的影响,为成肌细胞在神经再生领域的应用提供实验依据,并为进一步将其作为转基因载体研究打下基础. 方法 SD雄性大鼠20只按随机数字表法分为实验组和对照组,每组10只.建立大鼠腓肠肌神经植入术模型,实验组将原代培养的成肌细胞注射到神经植入术局部,对照组只注射同体积不含成肌细胞的培养基,通过胫神经功能指数(tibial functional index,TFI)测定、神经电生理检测、病理组织学检测,观察成肌细胞对神经植入术后神经肌肉功能恢复的影响. 结果 实验组胫神经功能恢复明显快于对照组(P＜0.01)；实验组大鼠腓肠肌神经电生理峰峰值(peak to peak value,PPV)、曲线下面积(area under the curve,AUC)、再生终板数量较对照组差异有统计学意义(P＜0.01). 结论 成肌细胞自体移植能使大鼠神经植入术后的神经肌肉功能恢复明显加快,再生终板数量增加.
目的 觀察成肌細胞自體移植對大鼠神經植入術後終闆再生及神經肌肉功能恢複的影響,為成肌細胞在神經再生領域的應用提供實驗依據,併為進一步將其作為轉基因載體研究打下基礎. 方法 SD雄性大鼠20隻按隨機數字錶法分為實驗組和對照組,每組10隻.建立大鼠腓腸肌神經植入術模型,實驗組將原代培養的成肌細胞註射到神經植入術跼部,對照組隻註射同體積不含成肌細胞的培養基,通過脛神經功能指數(tibial functional index,TFI)測定、神經電生理檢測、病理組織學檢測,觀察成肌細胞對神經植入術後神經肌肉功能恢複的影響. 結果 實驗組脛神經功能恢複明顯快于對照組(P＜0.01)；實驗組大鼠腓腸肌神經電生理峰峰值(peak to peak value,PPV)、麯線下麵積(area under the curve,AUC)、再生終闆數量較對照組差異有統計學意義(P＜0.01). 結論 成肌細胞自體移植能使大鼠神經植入術後的神經肌肉功能恢複明顯加快,再生終闆數量增加.
목적 관찰성기세포자체이식대대서신경식입술후종판재생급신경기육공능회복적영향,위성기세포재신경재생영역적응용제공실험의거,병위진일보장기작위전기인재체연구타하기출. 방법 SD웅성대서20지안수궤수자표법분위실험조화대조조,매조10지.건립대서비장기신경식입술모형,실험조장원대배양적성기세포주사도신경식입술국부,대조조지주사동체적불함성기세포적배양기,통과경신경공능지수(tibial functional index,TFI)측정、신경전생리검측、병리조직학검측,관찰성기세포대신경식입술후신경기육공능회복적영향. 결과 실험조경신경공능회복명현쾌우대조조(P＜0.01)；실험조대서비장기신경전생리봉봉치(peak to peak value,PPV)、곡선하면적(area under the curve,AUC)、재생종판수량교대조조차이유통계학의의(P＜0.01). 결론 성기세포자체이식능사대서신경식입술후적신경기육공능회복명현가쾌,재생종판수량증가.
Objective To observe the effects of autologous myoblast transplantation on endplate regeneration and neuromuscular function restoration following direct nerve implantation (DNI) in rats so as to offer experimental basis for the use of myoblasts in neural regeneration area and further lay foundation for the research using myoblasts as transgenic carriers. Methods A total of 20 male SD rats were randomly divided into experimental group and control group,with 10 rats in each group.Models of DNI in rat gastrocnemius were established.The experimental group was injected with primarily cultured autologous myoblasts to the DNI location,while the control group was injected with isometric medium without autologous myoblasts.The effects of myoblasts on the neuromuscular function recovery following DNI were studied by detecting the tibial functional index (FTI),neuro-electrophysiology and pathohistology.Results The experimental group displayed faster tibial nerve function recovery than the control group (P ＜ 0.01 ).The peak to peak value (PPV) of gastrocnemius neuro-electrophysiology,area under the curve and regenerated endplate number of the experimental group had statistical significances as compared with the control group ( P ＜ 0.01 ). Conclusion Myoblast autotransplantation accelerates the recoveryof neuromuscular function after rat DNI and increases the number of the regenerated endplates.
