中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2012年
11期
1042-1045
,共4页
王德亮%邢德国%吴建军%王亮%田克立%刘中浩%宫明智
王德亮%邢德國%吳建軍%王亮%田剋立%劉中浩%宮明智
왕덕량%형덕국%오건군%왕량%전극립%류중호%궁명지
骨髓祖代细胞%骨形态发生蛋白质类%糖尿病%基因转移技术
骨髓祖代細胞%骨形態髮生蛋白質類%糖尿病%基因轉移技術
골수조대세포%골형태발생단백질류%당뇨병%기인전이기술
Myeloid progenitor cells%Bone morphogenetic proteins%Diabetes mellitus%Gene transfer techniques
目的 观察骨形态发生蛋白-2(BMP-2)基因转染大鼠骨髓间充质干细胞(BMSCs)移植对糖尿病骨折愈合的影响,探讨糖尿病骨折治疗的新方法.方法 Wistar雄性成年6周龄大鼠50只,按随机数字表法分为对照组和实验组,均制成糖尿病骨折模型,体外高糖环境下腺病毒介导的BMP-2转染BMSCs,分别于骨折局部移植相应的BMSCs.术后1,2,3,4,6周行X线检查;取骨痂行HE染色,免疫组化检测骨痂中BMP-2的灰度值;ELISA法检测血清中BMP-2的浓度.结果 第4周实验组骨痂BMP-2灰度值为83±3,对照组为118±4(P＜0.01).第4周实验组血清BMP-2浓度为(203.80±8.96)ng/L,对照组为(139.15±4.19)ng/L(P＜0.01).结论 BMP-2基因转染的BMSCs移植能促进糖尿病大鼠骨折的愈合.
目的 觀察骨形態髮生蛋白-2(BMP-2)基因轉染大鼠骨髓間充質榦細胞(BMSCs)移植對糖尿病骨摺愈閤的影響,探討糖尿病骨摺治療的新方法.方法 Wistar雄性成年6週齡大鼠50隻,按隨機數字錶法分為對照組和實驗組,均製成糖尿病骨摺模型,體外高糖環境下腺病毒介導的BMP-2轉染BMSCs,分彆于骨摺跼部移植相應的BMSCs.術後1,2,3,4,6週行X線檢查;取骨痂行HE染色,免疫組化檢測骨痂中BMP-2的灰度值;ELISA法檢測血清中BMP-2的濃度.結果 第4週實驗組骨痂BMP-2灰度值為83±3,對照組為118±4(P＜0.01).第4週實驗組血清BMP-2濃度為(203.80±8.96)ng/L,對照組為(139.15±4.19)ng/L(P＜0.01).結論 BMP-2基因轉染的BMSCs移植能促進糖尿病大鼠骨摺的愈閤.
목적 관찰골형태발생단백-2(BMP-2)기인전염대서골수간충질간세포(BMSCs)이식대당뇨병골절유합적영향,탐토당뇨병골절치료적신방법.방법 Wistar웅성성년6주령대서50지,안수궤수자표법분위대조조화실험조,균제성당뇨병골절모형,체외고당배경하선병독개도적BMP-2전염BMSCs,분별우골절국부이식상응적BMSCs.술후1,2,3,4,6주행X선검사;취골가행HE염색,면역조화검측골가중BMP-2적회도치;ELISA법검측혈청중BMP-2적농도.결과 제4주실험조골가BMP-2회도치위83±3,대조조위118±4(P＜0.01).제4주실험조혈청BMP-2농도위(203.80±8.96)ng/L,대조조위(139.15±4.19)ng/L(P＜0.01).결론 BMP-2기인전염적BMSCs이식능촉진당뇨병대서골절적유합.
Objective To observe the effects of transplantation of bone marrow stem cells(BMSCs)transfected with bone morphogenetic protein-2(BMP-2)on fracture healing in rats with diabetes so as to provide a new therapy for diabetic fractures.Methods Fifty male adult Wistar rats aged six weeks randomized to the control group and experimental group were all employed to establish models with diabetic fractures.Under high glucose condition,BMSCs were transfected with BMP-2 by adenovirus vector in vitro.BMSCs transfected by BMP-2 were transplanted into the fracture area of rats in the experimental group,while non-transfected BMSCs into the corresponding area of rats in the control group.X-ray examination was performed at 1,2,3,4 and 6 weeks after transplantation.Bony calluses were collected for HE staining and gray scales of BMP-2 in calluses were determined by immunohistochemical method.Meanwhile,serum levels of BMP-2 were measured by ELtSA.Results The gray scales of BMP-2 in the calluses were 83±3 in the experimental group and 118±4 in the control group at four weeks(P＜0.01).The serum concentrations of BMP-2 were(203.80±8.96)ng/L in the experimental group and(139.15±4.19)ng/L in the control group at four weeks(P＜0.01).Conclusion Transplantation of BMSCs transfected by BMP-2 promotes fracture healing in diabetic rats.
