中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2013年
5期
456-460
,共5页
罗羲%费军%杜顺华%黄显凯%倪卫东
囉羲%費軍%杜順華%黃顯凱%倪衛東
라희%비군%두순화%황현개%예위동
感染%人工关节%防御素类%诊断
感染%人工關節%防禦素類%診斷
감염%인공관절%방어소류%진단
Infection%Joint prosthesis%Defensins%Diagnosis
目的 观察人β防御素-3(human beta-defensin-3,HBD-3)在人工关节感染(periprosthetic joint infection,PJI)患者假体周围组织中的表达,探讨其对PJI诊断与治疗的价值.方法 按临床诊断收集术中切取的假体周围组织和正常滑膜,并分为以下四组:PJI组(13例)、无菌性松动组(松动组,9例)、占位器治疗组(治疗组,12例)和正常组(15例).采用HE染色观察炎性细胞浸润,免疫荧光染色观察阳性细胞数及强度并采用Image-pro plus(IPP)7.0C软件测量平均吸光度值.收集术前外周血白细胞计数、红细胞沉降率(erythrocyte sedimentation rate,ESR)、C反应蛋白(C-reactive protein,CRP)结果.结果 HE染色正常组无炎性细胞浸润,其余三组有不同程度炎性细胞浸润.PJI组免疫荧光染色阳性细胞最多、阳性程度最强.PJI组平均吸光度值最高(0.430 ±0.013),其次为松动组(0.308±0.005)、治疗组(0.234±0.009)及正常组(0.089±0.019).术前PJI组外周血白细胞计数、ESR和CRP最高,其余三组差异无统计学意义.结论 HBD-3在PJI和假体无菌性松动患者的假体周围组织中存在高表达,对于PJI诊断及其与假体无菌性松动的鉴别是一种较好的指标.
目的 觀察人β防禦素-3(human beta-defensin-3,HBD-3)在人工關節感染(periprosthetic joint infection,PJI)患者假體週圍組織中的錶達,探討其對PJI診斷與治療的價值.方法 按臨床診斷收集術中切取的假體週圍組織和正常滑膜,併分為以下四組:PJI組(13例)、無菌性鬆動組(鬆動組,9例)、佔位器治療組(治療組,12例)和正常組(15例).採用HE染色觀察炎性細胞浸潤,免疫熒光染色觀察暘性細胞數及彊度併採用Image-pro plus(IPP)7.0C軟件測量平均吸光度值.收集術前外週血白細胞計數、紅細胞沉降率(erythrocyte sedimentation rate,ESR)、C反應蛋白(C-reactive protein,CRP)結果.結果 HE染色正常組無炎性細胞浸潤,其餘三組有不同程度炎性細胞浸潤.PJI組免疫熒光染色暘性細胞最多、暘性程度最彊.PJI組平均吸光度值最高(0.430 ±0.013),其次為鬆動組(0.308±0.005)、治療組(0.234±0.009)及正常組(0.089±0.019).術前PJI組外週血白細胞計數、ESR和CRP最高,其餘三組差異無統計學意義.結論 HBD-3在PJI和假體無菌性鬆動患者的假體週圍組織中存在高錶達,對于PJI診斷及其與假體無菌性鬆動的鑒彆是一種較好的指標.
목적 관찰인β방어소-3(human beta-defensin-3,HBD-3)재인공관절감염(periprosthetic joint infection,PJI)환자가체주위조직중적표체,탐토기대PJI진단여치료적개치.방법 안림상진단수집술중절취적가체주위조직화정상활막,병분위이하사조:PJI조(13례)、무균성송동조(송동조,9례)、점위기치료조(치료조,12례)화정상조(15례).채용HE염색관찰염성세포침윤,면역형광염색관찰양성세포수급강도병채용Image-pro plus(IPP)7.0C연건측량평균흡광도치.수집술전외주혈백세포계수、홍세포침강솔(erythrocyte sedimentation rate,ESR)、C반응단백(C-reactive protein,CRP)결과.결과 HE염색정상조무염성세포침윤,기여삼조유불동정도염성세포침윤.PJI조면역형광염색양성세포최다、양성정도최강.PJI조평균흡광도치최고(0.430 ±0.013),기차위송동조(0.308±0.005)、치료조(0.234±0.009)급정상조(0.089±0.019).술전PJI조외주혈백세포계수、ESR화CRP최고,기여삼조차이무통계학의의.결론 HBD-3재PJI화가체무균성송동환자적가체주위조직중존재고표체,대우PJI진단급기여가체무균성송동적감별시일충교호적지표.
Objective To observe expression of human beta-defensin-3 (HBD-3) in tissues around the infected artificial prostheses and investigate its value in treatment and diagnosis of periprosthetic joint infection (PJI).Methods According to clinical diagnosis,periprosthetic tissues and normal synovial membrane excised in operation were collected and divided into the following four groups:PJI group (n =13),aseptic loosening group (loosening group,n =9),spacer treatment group (treatment group,n =12),and normal group (n =15).HE staining was used to observe infiltration of inflammatory cells.Immunofluorescence staining was used to detect positive cells number and fluorescence intensity.Image-pro plus (IPP) 7.0C software was used to measure the average value of absorbance.Preoperative peripheral white blood cell count,erythrocyte sedimentation rate (ESR),and C-reactive protein (CRP) results were documented.Then,differences of those parameters were analyzed and compared among groups.Results HE staining revealed that all groups had different degree of inflammatory cell infiltration except for normal group.Immunofluorescence staining revealed that the most number of positive cells and highest fluorescence intensity existed in PJI group.Value of absorbance in PJI group was 0.430 ± 0.013,followed by 0.308 ±0.005 in loosening group,0.234 ± 0.009 in treatment group,and 0.089 ± 0.019 in normal group.Preoperative peripheral white blood cell count,ESR and CRP were the highest in PFI group,but were not significantly different among the remaining three groups.Conclusion HBD-3 is highly expressed in tissues around the prostheses which had infection or aseptic loosening,but its expression in response to infection and loosening has difference.
