CAJ | 학술논문

目的观察骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,bMSCs)移植对兔前交叉韧带(anterior cruciate ligament,ACL)重建后移植物与骨道愈合的影响.方法选择24只新西兰大白兔,取自体同侧部分腓肠肌腱作为移植物,构建ACL重建模型,双后肢均纳入研究,一侧使用混合bMSCs的纤维蛋白胶包裹移植物(bMSCs组),对侧后肢单纯使用纤维蛋白胶包裹移植物(对照组).分别于术后2,4,6,8周取材检测腱-骨界面的断裂强度和刚度. 结果 bMSCs组和对照组腱-骨界面的断裂强度和刚度均随修复时间延长呈上升趋势,其中实验组腱-骨界面的断裂强度和刚度自第6周始明显高于对照组(P＜0.05). 结论 bMSCs移植可明显提高兔ACL重建后早期腱-骨界面的断裂强度和刚度,促进移植物与骨道早期愈合.
목적 관찰골수간충질간세포(bone marrow-derived mesenchymal stem cells,bMSCs)이식대토전교차인대(anterior cruciate ligament,ACL)중건후이식물여골도유합적영향.방법 선택24지신서란대백토,취자체동측부분비장기건작위이식물,구건ACL중건모형,쌍후지균납입연구,일측사용혼합bMSCs적섬유단백효포과이식물(bMSCs조),대측후지단순사용섬유단백효포과이식물(대조조).분별우술후2,4,6,8주취재검측건-골계면적단렬강도화강도. 결과 bMSCs조화대조조건-골계면적단렬강도화강도균수수복시간연장정상승추세,기중실험조건-골계면적단렬강도화강도자제6주시명현고우대조조(P＜0.05). 결론 bMSCs이식가명현제고토ACL중건후조기건-골계면적단렬강도화강도,촉진이식물여골도조기유합.
Objective To observe the effect of bone marrow-derived mesenchymal stem cells (bMSCs) on graft healing within a bone tunnel after anterior cruciate ligament (ACL) reconstruction in rabbits.Methods The study involved 24 New Zealand white rabbits undergone ACL reconstruction with an autologous ipsilateral gastrocnemius tendon graft.Both hindlimbs were included.In one hindlimb,graft coated with fibrin glue compound by bMSCs was employed (bMSCs group).Whereas in the contralateral hindlimb,graft coated with fibrin glue without cells was employed (control group).At postoperative 2,4,6 and 8 weeks,specimens were harvested to have a biomechanical test of tensile strength and stiffness of tendon-bone interface.Results Tensile strength and stiffness of tendon-bone interface in both experiment and control groups presented a rising trend with the prolong of repair time.In contrast,significantly higher tensile strength and stiffness of tendon-bone interface were observed in experiment group since the 6 weeks (P ＜ 0.05).Conclusion bMSCs transplantation significantly enhances the early tensile strength and stiffness at tendon-bone interface after ACL reconstruction in rabbits and improves the graft healing within a bone tunnel.