CAJ | 학술논문

目的探讨端粒酶反转录酶(telomerase reverse transcriptase,TERT)基因的表达与星形胶质细胞活化的相关性. 方法 20只雄性SD大鼠(新生3d)培养的星形胶质细胞,按随机数字表法分为4组:A组为活化未转染星形胶质细胞,B组为活化转染星形胶质细胞,C组为未活化星形胶质细胞,D组为活化空白质粒转染星形胶质细胞,每组5只.通过细胞增殖试剂盒(cell counting kit-8,CCK-8)检测各组细胞的增殖率;免疫细胞化学法检测TERT的表达;RTPCR法检测各组细胞TERT、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)基因的表达.结果 B组星形胶质细胞增殖能力显著低于A、D、C组(F =43.418,P＜0.01).TERT mRNA和GFAP mRNA在A、D组中的表达明显高于B、C组,A、D组比较差异无统计学意义;在A、D组中二者呈线性关系(r =0.701,0.704,P＜0.01);B、C组中二者未见明显相关线性关系(r=0.260,P＞0.05).TERT蛋白和GFAP蛋白在A、D组中的表达明显高于B、C组,A、D组比较差异无统计学意义. 结论 TERT基因参与了星形胶质细胞的活化,有促进星形胶质细胞活化的作用.
목적 탐토단립매반전록매(telomerase reverse transcriptase,TERT)기인적표체여성형효질세포활화적상관성. 방법 20지웅성SD대서(신생3d)배양적성형효질세포,안수궤수자표법분위4조:A조위활화미전염성형효질세포,B조위활화전염성형효질세포,C조위미활화성형효질세포,D조위활화공백질립전염성형효질세포,매조5지.통과세포증식시제합(cell counting kit-8,CCK-8)검측각조세포적증식솔;면역세포화학법검측TERT적표체;RTPCR법검측각조세포TERT、효질섬유산성단백(glial fibrillary acidic protein,GFAP)기인적표체.결과 B조성형효질세포증식능력현저저우A、D、C조(F =43.418,P＜0.01).TERT mRNA화GFAP mRNA재A、D조중적표체명현고우B、C조,A、D조비교차이무통계학의의;재A、D조중이자정선성관계(r =0.701,0.704,P＜0.01);B、C조중이자미견명현상관선성관계(r=0.260,P＞0.05).TERT단백화GFAP단백재A、D조중적표체명현고우B、C조,A、D조비교차이무통계학의의. 결론 TERT기인삼여료성형효질세포적활화,유촉진성형효질세포활화적작용.
Objective To investigate the relationship between telomerase reverse transcriptase (TERT) gene expression and astrocyte activation.Methods Twenty neonatal 3-day-old male SD rats were used for culture of the astrocytes.The astrocytes were divided into Group A (activated,non-transfected astrocytes),Group B (activated,transfected astrocytes),Group C (unactivated astrocytes) and Group D (activated,empty plasmid transfected astrocytes) according to the random number table,with 5 rats per group.The cell proliferation rate in each group was detected by cell counting kit-8 (CCK-8) ;TERT expression by immunocytochemical method; expressions of TERT and glial fibrillary acidic protein (GFAP) genes by RT-PCR assay.Results Astrocytic proliferation ability in Group B lowered significantly as compared with that in Groups A,D and C (F =43.418,P ＜ 0.01).Expressions of TERT and GFAP mRNAs in Groups A and D were significantly higher than those in Group B and C,and no significant difference was found between Groups A and D.Besides,there was a linear correlation between mRNAs expressions of both genes in Groups A and D (r =0.701,0.704,P ＜ 0.01),while no significant linear correlation was observed in Groups B and C (r =0.260,P ＞ 0.05).Expressions of TERT and GFAP proteins in Groups A and D were markedly higher than those in Groups B and C and no significant difference was found between Groups A and D.Conclusion TERT genes are involved in the activation of astrocytes and exert effect on promoting the activation of astrocytes.