CAJ | 학술논문

目的探讨人羊膜上皮细胞(human amniotic epithelial cell,hAECs)在移植治疗兔脑出血中的疗效及作用机制. 方法选取30只新西兰大白兔制作兔脑出血模型,按随机数字表法分为hAECs移植组及等渗盐水对照组,每组15只.将携带绿色荧光蛋白(green fluorescent protein,GFP)的反转录病毒转染hAECs,转染后的hAECs移植入实验组中,定期观察hAECs移植组及等渗盐水对照组的形态学、行为学,并检测移植的hAECs存活情况及移植灶周组织的胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和神经元微管相关蛋白2(microtubule-associated protein 2,MAP-2)的表达情况. 结果 hAECs移植组行走、支撑和协调运动等功能逐步恢复,恢复期多在2～3周;hAECs移植组肢体运动功能大多恢复为Ⅱ～Ⅲ级,而等渗盐水对照组肢体运动功能恢复缓慢,大多为Ⅰ～Ⅱ级.肢体活动功能hAECs移植组与等渗盐水对照组Tarlov评分差异有统计学意义(P＜0.05).hAECs移植组神经组织GFAP、MAP-2免疫组化染色均呈阳性表达,而等渗盐水对照组未见表达(P＜0.05). 结论 hAECs移植治疗家兔脑出血能有效改善神经行为学功能,减轻神经功能损伤.
목적 탐토인양막상피세포(human amniotic epithelial cell,hAECs)재이식치료토뇌출혈중적료효급작용궤제. 방법 선취30지신서란대백토제작토뇌출혈모형,안수궤수자표법분위hAECs이식조급등삼염수대조조,매조15지.장휴대록색형광단백(green fluorescent protein,GFP)적반전록병독전염hAECs,전염후적hAECs이식입실험조중,정기관찰hAECs이식조급등삼염수대조조적형태학、행위학,병검측이식적hAECs존활정황급이식조주조직적효질섬유산성단백(glial fibrillary acidic protein,GFAP)화신경원미관상관단백2(microtubule-associated protein 2,MAP-2)적표체정황. 결과 hAECs이식조행주、지탱화협조운동등공능축보회복,회복기다재2～3주;hAECs이식조지체운동공능대다회복위Ⅱ～Ⅲ급,이등삼염수대조조지체운동공능회복완만,대다위Ⅰ～Ⅱ급.지체활동공능hAECs이식조여등삼염수대조조Tarlov평분차이유통계학의의(P＜0.05).hAECs이식조신경조직GFAP、MAP-2면역조화염색균정양성표체,이등삼염수대조조미견표체(P＜0.05). 결론 hAECs이식치료가토뇌출혈능유효개선신경행위학공능,감경신경공능손상.
Objective To investigate the effect and mechanism of human amniotic epithelial cells (hAECs) transplantation in treatment of cerebral hemorrhage in rabbits.Methods Thirty New Zealand white rabbits were used to induce cerebral hemorrhage.Animals were divided into hAECs group and isotonic saline group according to random number table,with 15 rabbits per group.Before transplanted to rabbits,hAECs were transfected with the retrovirus carrying green fluorescent protein (GFP).Morphologic and behavioral changes in both groups were noted periodically.Survival of transplanted hAECs and expressions of glial fibrillary acidic protein (GFAP) and microtubule-associated protein 2 (MAP-2)in focal cerebral tissues were observed.Results In hAECs group,the rabbits obtained progressive recovery in walking,supporting and coordinated motion.Restoration period mostly ranged from 2-3 weeks.Most of the rabbits in hAECs group had limb motor function recovered to grade Ⅱ-Ⅲ,while the recovery is slow in isotonic saline group with most ranging from grade Ⅰ-Ⅱ.According to Tarlov score,limb motor function presented significant difference between hAECs group and isotonic saline group (P ＜ 0.05).Immunohistochemical staining showed positive expressions of GFAP and MAP-2 in hAECs group,but no expressions in isotonic saline group (P ＜ 0.05).Conclusion hAECs transplantation effectively improves neural behavior and reduces nerve function impairment in treatment of cerebral hemorrhage in rabbits.