CAJ | 학술논문

目的观察携神经干细胞(neural stem cells,NSCs)的肌基膜管对脊髓损伤的修复作用. 方法选择同一窝别SD大鼠36只,制备脊髓半切损伤模型,按随机数字表法分为空白对照组(脊髓断端处仅做等渗盐水冲洗)、NSCs组(脊髓断端处注射NSCs)和NSCs+肌基膜管组(脊髓断端处植入NSCs+肌基膜管复合体),每组12只.4,8周后观察移植细胞的存活、迁移、肌基膜管与宿主的组织相容性,采用BBB评分法观察术后2,4,8周各时相点大鼠后肢运动功能恢复情况. 结果 NSCs+肌基膜管组NSCs能够在断端正常生长,并向断端头尾处迁移且与周围脊髓组织融合,而NSCs组断端处NSCs存活数量较少且炎症细胞浸润明显.术后2周BBB评分各组比较差异无统计学意义,术后4,8周BBB评分NSCs+肌基膜管组分别为7.92±1.00,11.38±1.51,空白对照组分别为3.82±0.75,3.71±0.76,NSCs组分别为6.25±1.06,8.25±1.83,NSCs+肌基膜管组明显高于空白对照组和NSCs组(P＜0.05). 结论肌基膜管能够提供NSCs沿其管腔定向生长基础,同时能够促进宿主自身细胞沿管腔内的基质成分爬行,减少局部炎症反应.
목적 관찰휴신경간세포(neural stem cells,NSCs)적기기막관대척수손상적수복작용. 방법 선택동일와별SD대서36지,제비척수반절손상모형,안수궤수자표법분위공백대조조(척수단단처부주등삼염수충세)、NSCs조(척수단단처주사NSCs)화NSCs+기기막관조(척수단단처식입NSCs+기기막관복합체),매조12지.4,8주후관찰이식세포적존활、천이、기기막관여숙주적조직상용성,채용BBB평분법관찰술후2,4,8주각시상점대서후지운동공능회복정황. 결과 NSCs+기기막관조NSCs능구재단단정상생장,병향단단두미처천이차여주위척수조직융합,이NSCs조단단처NSCs존활수량교소차염증세포침윤명현.술후2주BBB평분각조비교차이무통계학의의,술후4,8주BBB평분NSCs+기기막관조분별위7.92±1.00,11.38±1.51,공백대조조분별위3.82±0.75,3.71±0.76,NSCs조분별위6.25±1.06,8.25±1.83,NSCs+기기막관조명현고우공백대조조화NSCs조(P＜0.05). 결론 기기막관능구제공NSCs연기관강정향생장기출,동시능구촉진숙주자신세포연관강내적기질성분파행,감소국부염증반응.
Objective To observe effect of muscle basal lamina containing neural stem cells (NSCs) in repair of spinal cord injury.Methods Thirty-six SD rats from the same nest were used in the study and spinal cord hemisection models were induced.The animals were classified to blank control group (clearance of the lesion edge only with isotonic saline),NSCs group (transplantation of NSCs to the edge),NSCs + muscle basal lamina group (transplantation of complex of NSCs and muscle basal lamina to the edge) according to random number table,with 12 rats per group.At weeks 4 and 8,survival and migration of the transplanted cells and compatibility of muscle basal lamina with the host were detected.At weeks 2,4,and 8,the hindlimb function was assayed using BBB scoring system.Results NSCs in NSCs + muscle basal lamina group grew at the lesion edge,migrated to both sides of the edge,and integrated with peripheral tissues.Whereas,few NSCs survived at the lesion edge in NSCs group and inflammatory cell infiltration was notable.At week 2,there was no statistical difference of BBB score among the three groups.At weeks 4 and 8,BBB score in NSCs + muscle basal lamina group (7.92 ± 1.00,11.38 ± 1.51) was significantly higher than that in blank control group (3.82 ± 0.75,3.71 ± 0.76) and NSCs group (6.25 ±1.06,8.25 ± 1.83) (P＜0.05).Conclusion Muscle basal lamina orients growth of NSCs along its lumen,facilitates migration of host cells to ground substance within its lumen,and reduces local inflammatory reaction.