中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2014年
5期
464-466
,共3页
朱克军%黄洪%储辉%罗志勇%徐志明%俞航%张世明
硃剋軍%黃洪%儲輝%囉誌勇%徐誌明%俞航%張世明
주극군%황홍%저휘%라지용%서지명%유항%장세명
脑损伤%缺血预处理%细胞间黏附分子
腦損傷%缺血預處理%細胞間黏附分子
뇌손상%결혈예처리%세포간점부분자
Brain injuries%Ischemic preconditioning%Cell adhesion molecules
目的 探讨缺血预处理(ischemic precoudition,IPC)对大鼠创伤性脑损伤(traumatic brain injury,TBI)后脑组织细胞间黏附分子-1(intracellular adhesion molecule-1,ICAM-1)表达的影响. 方法 雄性SD大鼠60只,体重220 ～250 g,按随机数字表法分为假手术组、TBI组和IPC组,每组20只.短暂夹闭双侧颈总动脉制备脑IPC模型,采用Feeney自由落体撞击法制作TBI模型,假手术组仅行右侧顶部开窗而无TBI.分别于TBI后6,72 h处死10只大鼠,取损伤脑组织,计算脑组织湿/干重比(W/D)值,采用免疫组化法测定ICAM-1表达水平,光镜观察病理学结果. 结果 与假手术组比较,TBI组脑组织W/D值升高(6 h:4.2±0.4比2.7±0.4;72 h:5.0±0.1比3.1±0.2,P＜0.05),脑组织ICAM-1表达上调(6 h:25.4 ±3.5比8.6±1.3;72 h:36.5 ±5.4比8.4±1.6,P＜0.05);与TBI组比较,IPC组脑组织W/D值降低(6 h:3.5±0.6比4.2±0.4;72 h:3.7±0.4比5.0±0.1,P ＜0.05),脑组织ICAM-1表达下调(6 h:16.5±2.7比25.4 ±3.5;72 h:24.3 ±4.6比36.5 ±5.4,P＜0.05).IPC组脑组织损伤程度轻于TBI组. 结论 IPC可以下调脑组织ICAM-1表达,从而减轻大鼠TBI.
目的 探討缺血預處理(ischemic precoudition,IPC)對大鼠創傷性腦損傷(traumatic brain injury,TBI)後腦組織細胞間黏附分子-1(intracellular adhesion molecule-1,ICAM-1)錶達的影響. 方法 雄性SD大鼠60隻,體重220 ～250 g,按隨機數字錶法分為假手術組、TBI組和IPC組,每組20隻.短暫夾閉雙側頸總動脈製備腦IPC模型,採用Feeney自由落體撞擊法製作TBI模型,假手術組僅行右側頂部開窗而無TBI.分彆于TBI後6,72 h處死10隻大鼠,取損傷腦組織,計算腦組織濕/榦重比(W/D)值,採用免疫組化法測定ICAM-1錶達水平,光鏡觀察病理學結果. 結果 與假手術組比較,TBI組腦組織W/D值升高(6 h:4.2±0.4比2.7±0.4;72 h:5.0±0.1比3.1±0.2,P＜0.05),腦組織ICAM-1錶達上調(6 h:25.4 ±3.5比8.6±1.3;72 h:36.5 ±5.4比8.4±1.6,P＜0.05);與TBI組比較,IPC組腦組織W/D值降低(6 h:3.5±0.6比4.2±0.4;72 h:3.7±0.4比5.0±0.1,P ＜0.05),腦組織ICAM-1錶達下調(6 h:16.5±2.7比25.4 ±3.5;72 h:24.3 ±4.6比36.5 ±5.4,P＜0.05).IPC組腦組織損傷程度輕于TBI組. 結論 IPC可以下調腦組織ICAM-1錶達,從而減輕大鼠TBI.
목적 탐토결혈예처리(ischemic precoudition,IPC)대대서창상성뇌손상(traumatic brain injury,TBI)후뇌조직세포간점부분자-1(intracellular adhesion molecule-1,ICAM-1)표체적영향. 방법 웅성SD대서60지,체중220 ～250 g,안수궤수자표법분위가수술조、TBI조화IPC조,매조20지.단잠협폐쌍측경총동맥제비뇌IPC모형,채용Feeney자유락체당격법제작TBI모형,가수술조부행우측정부개창이무TBI.분별우TBI후6,72 h처사10지대서,취손상뇌조직,계산뇌조직습/간중비(W/D)치,채용면역조화법측정ICAM-1표체수평,광경관찰병이학결과. 결과 여가수술조비교,TBI조뇌조직W/D치승고(6 h:4.2±0.4비2.7±0.4;72 h:5.0±0.1비3.1±0.2,P＜0.05),뇌조직ICAM-1표체상조(6 h:25.4 ±3.5비8.6±1.3;72 h:36.5 ±5.4비8.4±1.6,P＜0.05);여TBI조비교,IPC조뇌조직W/D치강저(6 h:3.5±0.6비4.2±0.4;72 h:3.7±0.4비5.0±0.1,P ＜0.05),뇌조직ICAM-1표체하조(6 h:16.5±2.7비25.4 ±3.5;72 h:24.3 ±4.6비36.5 ±5.4,P＜0.05).IPC조뇌조직손상정도경우TBI조. 결론 IPC가이하조뇌조직ICAM-1표체,종이감경대서TBI.
Objective To investigate the effect of ischemic preconditioning (IPC) on expression of intracellular adhesion molecule-1 (ICAM-1) in brain tissues following traumatic brain injury (TBI) in rats.Methods Sixty male SD rats weighing 220-250 g were randomly divided into three groups (n =20 for each):sham operation group,TBI group,and IPC group.Cerebral IPC models were induced by transient occlusion of the bilateral common carotid arteries; TBI models were induced by Feeney's freefalling method; rats in sham operation group were only performed exposure of dura of the right parietal lobe.Ten rats were sacrificed respectively at 6 and 72 hours after TBI and injured brain tissues were harvested to estimate wet/dry weight (W/D) ratio for the brain,determine ICAM-1 expression by immunohistochemistry and perform microscopic examination.Results Brain W/D ratio was significantly increased in TBI group compared with sham operation group (6 h:4.2 ± 0.4 vs 2.7 ± 0.4 ; 72 h:5.0 ±0.1 vs 3.1 ± 0.2,P ＜ 0.05).ICAM-1 expression was up-regulated in TBI group compared with sham operationgroup (6h:25.4±3.5vs8.6±1.3; 72 h:36.5±5.4 vs8.4±1.6,P＜0.05).W/D ratio was significantly decreased in IPC group compared with TBI group (6 h:3.5 ±0.6 vs 4.2 ±0.4; 72 h:3.7 ± 0.4 vs 5.0 ± 0.1,P ＜ 0.05).ICAM-1 expression was down-regulated in IPC group compared with TBI group (6 h:16.5 ± 2.7 vs 25.4 ± 3.5 ; 72 h:24.3 ± 4.6 vs 36.5 ± 5.4,P ＜ 0.05).Milder injury to brain tissues was observed in IPC group than in TBI group.Conclusion IPC can attenuate TBI in rats by down-regulating the expression of ICAM-1.
