CAJ | 학술논문

目的研究脂肪间充质干细胞(adipose mesenchymal stem cells,ADMSCs)复合丝素膜在修复阴茎白膜缺损时纤维组织的重塑效果. 方法选择雄性新西兰大白兔56只建立白膜缺损模型,按随机数字表法分为四组:A组为丝素膜修补组,B组为ADMSCs丝素膜修补组,C组为睾丸鞘膜修补组,D组为对照组,每组14只.术后6,12周行组织学HE染色、天狼星红染色、Hart染色和巨噬细胞免疫荧光染色检查,观察各组修复情况. 结果术后12周HE染色显示,D组新生纤维组织排列紊乱,A、B、C组新生纤维组织排列整齐.天狼星红染色显示,Ⅰ型、Ⅲ型胶原纤维面积(以像素表示)A组为98 780 ±4 190,51 177 ±5 464;B组为94 855 ±9 010,50 815±3 895;C组为99 860 ±6 015,50 948±6 595;D组为79 386 ±2 237,85 278±2 645;其中A、B、C组胶原纤维以Ⅰ型为主,三组间差异无统计学意义,而D组Ⅰ型胶原纤维少于其他三组(P＜0.01),Ⅲ型胶原纤维多于其他三组(P＜0.01).Hart染色显示,A、B、C和D组弹性纤维面积(以像素表示)分别为2 805±90,2 849±84,3 068±485和1 961±96,B、C组差异无统计学意义,A组显著少于B、C组(P＜0.01),D组显著少于其他各组(P＜0.叭).术后6周巨噬细胞阳性数A、B、C和D组分别为4.10 ±0.87,3.80±0.78,3.70±0.94和6.80±1.63,A、B、C组均显著低于D组(P＜0.01),A、B、C组差异无统计学意义. 结论 ADMSCs丝素膜在修复兔阴茎白膜缺损时能达到与自体组织修复基本同等的效果.
목적 연구지방간충질간세포(adipose mesenchymal stem cells,ADMSCs)복합사소막재수복음경백막결손시섬유조직적중소효과. 방법 선택웅성신서란대백토56지건립백막결손모형,안수궤수자표법분위사조:A조위사소막수보조,B조위ADMSCs사소막수보조,C조위고환초막수보조,D조위대조조,매조14지.술후6,12주행조직학HE염색、천랑성홍염색、Hart염색화거서세포면역형광염색검사,관찰각조수복정황. 결과 술후12주HE염색현시,D조신생섬유조직배렬문란,A、B、C조신생섬유조직배렬정제.천랑성홍염색현시,Ⅰ형、Ⅲ형효원섬유면적(이상소표시)A조위98 780 ±4 190,51 177 ±5 464;B조위94 855 ±9 010,50 815±3 895;C조위99 860 ±6 015,50 948±6 595;D조위79 386 ±2 237,85 278±2 645;기중A、B、C조효원섬유이Ⅰ형위주,삼조간차이무통계학의의,이D조Ⅰ형효원섬유소우기타삼조(P＜0.01),Ⅲ형효원섬유다우기타삼조(P＜0.01).Hart염색현시,A、B、C화D조탄성섬유면적(이상소표시)분별위2 805±90,2 849±84,3 068±485화1 961±96,B、C조차이무통계학의의,A조현저소우B、C조(P＜0.01),D조현저소우기타각조(P＜0.팔).술후6주거서세포양성수A、B、C화D조분별위4.10 ±0.87,3.80±0.78,3.70±0.94화6.80±1.63,A、B、C조균현저저우D조(P＜0.01),A、B、C조차이무통계학의의. 결론 ADMSCs사소막재수복토음경백막결손시능체도여자체조직수복기본동등적효과.
Objective To investigate the effect of silk fibroin scaffolds seeded with adipose mesenchymal stem cells (ADMSCs) in remodeling fiber for tunica albuginea defect in rabbits.Methods Fifty-six New Zealand rabbits were divided into 4 groups according to the random number table after a defect was created in the tunica albuginea:Group A (the defect was repaired with silk fibroin),Group B (with silk fibroin seeded with ADMSCs),Group C (with autologous tunica vaginalis) and Group D (left unrepaired),with 14 rabbits per group.Tunica albuginea sections were obtained for HE staining,Sirius red staining,Hart staining and immunofluorescence staining of macrophages at 6 and 12 weeks after surgery.Results At 12 weeks after surgery,HE staining revealed chaotically distributed new fiber ingrowth in Group D and orderly ingrowth in Groups A,B,and C.At 12 weeks after surgery,Sirius red staining revealed mean area of type Ⅰ collagen fibers was greater than that of type Ⅲ in Groups A (98 780 ±4 190 vs 51 177 ±5 464),B(94 855 ±9 010 vs 50 815 ±3 895),and C(99 860 ±6 015 vs 50 948 ± 6 595),but the difference in area of collagen fiber of the same type was insignificant among the three Groups.Moreover,less type Ⅰ collagen fibers (79 386 ±2 237) and more type Ⅲ collagen fibers (85 278 ± 2 645) were observed in Group D compared with other three Groups (P ＜ 0.01).At 12 weeks after surgery,Hart staining showed the mean area of elastic fibers in Groups A,B,C,and D was 2 805 ± 90,2 849 ±84,3 068 ±485,and 1 961 ±96 respectively.There was no statistical difference between Groups B and C,but less amount of fibers was observed in Group A (P ＜ 0.01) and least amount was observed in Group D (P ＜0.01).At 6 weeks after surgery,the number of infiltrating macrophages in Groups A,B,C and D was 4.10 ± 0.87,3.80 ± 0.78,3.70 ± 0.94,and 6.80 ± 1.63 respectively.There was no statistical difference in the number of macrophage infiltration among Groups A,B and C,but all were lower than that in Group D (P ＜ 0.01).Conclusion Silk fibroin seeded with ADMSCs is comparable to autologous grafts for repair of tunica albuginea defect in rabbits.