中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2014年
8期
827-830
,共4页
脑损伤%脑组织%免疫球蛋白类
腦損傷%腦組織%免疫毬蛋白類
뇌손상%뇌조직%면역구단백류
Brain injuries%Brain tissues%Immunoglobulins
目的 探讨大鼠脑挫伤后损伤侧与非损伤侧脑组织病理变化及其意义. 方法 选择健康成年Wistar大鼠100只,按随机数字表法分为假手术组和挫伤组,各组又分为1,3,6,12,24 h5个时相点,每个时相点10只大鼠.各时相点取脑组织分别行挫伤处及非挫伤处病理形态学观察,IgG免疫组化染色半定量分析检测血脑屏障(blood brain barrier,BBB)通透性. 结果 假手术组双侧脑组织结构未见异常,IgG染色阴性.挫伤组损伤侧伤后1h即出现以BBB破坏为特征的血管源性水肿,3h出现细胞内水肿,两种水肿均随时间延长逐渐加重.挫伤后12 h后开始出现组织坏死、炎性细胞浸润及小胶质细胞增生.伤后1 h IgG开始呈阳性,6h最强,维持高水平至24 h.而挫伤组非损伤侧伤后1h结构未见异常,IgG染色阴性.3h后出现细胞内水肿,IgG染色弱阳性.6h后细胞内水肿加重,同时出现血管源性水肿,IgG染色呈强阳性.12～24 h细胞内水肿仍继续加重,而血管源性水肿有缓解趋势,IgG染色减弱,并见小胶质细胞增生. 结论 脑挫伤后损伤侧与非损伤侧病理改变有差异,可为脑挫伤治疗方法选择提供依据.
目的 探討大鼠腦挫傷後損傷側與非損傷側腦組織病理變化及其意義. 方法 選擇健康成年Wistar大鼠100隻,按隨機數字錶法分為假手術組和挫傷組,各組又分為1,3,6,12,24 h5箇時相點,每箇時相點10隻大鼠.各時相點取腦組織分彆行挫傷處及非挫傷處病理形態學觀察,IgG免疫組化染色半定量分析檢測血腦屏障(blood brain barrier,BBB)通透性. 結果 假手術組雙側腦組織結構未見異常,IgG染色陰性.挫傷組損傷側傷後1h即齣現以BBB破壞為特徵的血管源性水腫,3h齣現細胞內水腫,兩種水腫均隨時間延長逐漸加重.挫傷後12 h後開始齣現組織壞死、炎性細胞浸潤及小膠質細胞增生.傷後1 h IgG開始呈暘性,6h最彊,維持高水平至24 h.而挫傷組非損傷側傷後1h結構未見異常,IgG染色陰性.3h後齣現細胞內水腫,IgG染色弱暘性.6h後細胞內水腫加重,同時齣現血管源性水腫,IgG染色呈彊暘性.12～24 h細胞內水腫仍繼續加重,而血管源性水腫有緩解趨勢,IgG染色減弱,併見小膠質細胞增生. 結論 腦挫傷後損傷側與非損傷側病理改變有差異,可為腦挫傷治療方法選擇提供依據.
목적 탐토대서뇌좌상후손상측여비손상측뇌조직병리변화급기의의. 방법 선택건강성년Wistar대서100지,안수궤수자표법분위가수술조화좌상조,각조우분위1,3,6,12,24 h5개시상점,매개시상점10지대서.각시상점취뇌조직분별행좌상처급비좌상처병리형태학관찰,IgG면역조화염색반정량분석검측혈뇌병장(blood brain barrier,BBB)통투성. 결과 가수술조쌍측뇌조직결구미견이상,IgG염색음성.좌상조손상측상후1h즉출현이BBB파배위특정적혈관원성수종,3h출현세포내수종,량충수종균수시간연장축점가중.좌상후12 h후개시출현조직배사、염성세포침윤급소효질세포증생.상후1 h IgG개시정양성,6h최강,유지고수평지24 h.이좌상조비손상측상후1h결구미견이상,IgG염색음성.3h후출현세포내수종,IgG염색약양성.6h후세포내수종가중,동시출현혈관원성수종,IgG염색정강양성.12～24 h세포내수종잉계속가중,이혈관원성수종유완해추세,IgG염색감약,병견소효질세포증생. 결론 뇌좌상후손상측여비손상측병리개변유차이,가위뇌좌상치료방법선택제공의거.
Objective To investigate the pathological changes of brain tissues from contused and contralateral non-contused sides and their significance.Methods A total of 100 healthy adult Wistar rats were assigned to brain contusion group and sham operation group according to the random number table.Ten rats in each group were sacrificed at 1,3,6,12,and 24 hours respectively.Brain samples were collected to perform pathophysiological analysis of brain tissues and test blood brain barrier (BBB)permeability by semiquantitative immunohistochemical staining of IgG.Results There was no damage to the bilateral brain tissues in sham operation group and IgG stain was negative.In brain contusion group,angioedema characterized by the breakdown of BBB was seen in the contused side at 1 hour followed by cellular edema at 3 hours,with aggravation of both over time.Moreover,tissue necrosis,inflammatory cell infiltration,and microglia proliferation emerged at 12 hours.Besides,IgG-positive staining was seen at 1 hour,was strongest at 6 hours,and remained a high level at 24 hours.With respect to the non-injured side in brain contusion group,no pathological abnormalities and negatively stained IgG were observed at 1 hour; cellular edema and weakly positive-stained IgG were found at 3 hours; aggravated cellular edema,emergence of angioedema,and IgG strongly positive staining were noted at 6 hours;cellular edema continued aggravation,but angioedema tended to be attenuated,IgG staining weakened,and microglia proliferation were observed at 12-24 hours.Conclusion Pathological changes of brain tissues from the contused and contralateral non-contused sides are differed,which provides a basis in determining treatment choices.
